Efecto in vitro del extracto hidro etanólico de hojas y flores de Nerium oleander sobre el crecimiento de Lasiodiplodia theobromae
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue evaluar el efecto del extracto hidro etanólico de hojas y flores de Nerium oleander sobre el crecimiento del hongo fitopatógeno Lasiodiplodia theobromae in vitro. El proceso comenzó con la extracción por maceración del extracto hidro etanólico de hojas y flores y posterior rota evaporación. Luego, se incorporaron en el agar sabouraud en cuatro concentraciones distintas (15, 30, 60 y 100 mg/mL), se sirvieron en placas Petri, se dejaron solidificar y se sembraron con L. theobromae a 28° C por 72 horas. Los diámetros de crecimiento se midieron a partir 24 horas posteriores a la siembra. Los resultados obtenidos se expresaron en porcentaje de crecimiento y fueron evaluados por análisis de varianza (ANOVA) y Post-Hoc mediante HSD Tukey, demostrando que existen diferencias significativas entre las concentraciones evaluadas. Se observó que a diferencia de las concentraciones evaluadas del extracto hidro etanólico de hojas, las concentraciones de 60 y 100 mg/mL del extracto hidroetanólico de flores permitió un crecimiento de 54 y 52% respectivamente, generando una mayor inhibición
The present research aimed to evaluate the effect of the hydroethanolic extract of Nerium oleander leaves and flowers on the growth of the phytopathogenic fungus Lasiodiplodia theobromae in vitro. The process began with the extraction by maceration of the hydroethanolic extract of leaves and flowers and subsequent rotary evaporation. Then, they were incorporated into sabouraud agar at four different concentrations (15, 30, 60, and 100 mg/mL), served in Petri dishes, solidified, and sown with L. theobromae at 28° C for 72 hours. The growth diameters were determined 24 hours after sowing. The results obtained were expressed as percentages of growth and they were evaluated by Analysis of Variance (ANOVA) and Post-Hoc using HSD Tukey, demonstrating that there are significant differences between the concentrations evaluated. It was observed that, unlike the evaluated concentrations of the hydroethanolic extract of leaves, the concentrations of 60 and 100 mg/mL of the hydroethanolic extract of flowers allowed growth of 54 and 52%, respectively, generating greater inhibition.
The present research aimed to evaluate the effect of the hydroethanolic extract of Nerium oleander leaves and flowers on the growth of the phytopathogenic fungus Lasiodiplodia theobromae in vitro. The process began with the extraction by maceration of the hydroethanolic extract of leaves and flowers and subsequent rotary evaporation. Then, they were incorporated into sabouraud agar at four different concentrations (15, 30, 60, and 100 mg/mL), served in Petri dishes, solidified, and sown with L. theobromae at 28° C for 72 hours. The growth diameters were determined 24 hours after sowing. The results obtained were expressed as percentages of growth and they were evaluated by Analysis of Variance (ANOVA) and Post-Hoc using HSD Tukey, demonstrating that there are significant differences between the concentrations evaluated. It was observed that, unlike the evaluated concentrations of the hydroethanolic extract of leaves, the concentrations of 60 and 100 mg/mL of the hydroethanolic extract of flowers allowed growth of 54 and 52%, respectively, generating greater inhibition.
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Keywords
Lasiodiplodia theobromae, Nerium oleander, Extracto hidro etanólico.