Genotipificación de plasmodium vivax de pacientes con malaria, de bellavista, sullana, Perú, usando el gen msp3a
Murga Gutierrez, Santos Nélida
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Murga Gutiérrez SN. Genotyping of Plasmodium vivax from patients with malaria, in Bellavista, Sullana, Peru, using the MSP3α gene (doctoral thesis). Trujillo: Universidad Nacional de Trujillo; 2010. The aim of the study was to genotype isolates of P. vivax from patients with malaria, in the Bellavista District, Sullana, Peru, using the gene encoding the merozoite surface protein-3 (MSP3α), and a combined polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) protocol. Fifty seven blood samples were obtained between July 2009 and January 2010, from patients with malaria caused by P. vivax diagnosed by thick smear examination, which also identified the parasite density and hematocrit indicator of anemia. This diagnosis was confirmed by nested PCR, and in 96.5% (55/57) of them the species was reconfirmed. Not one patient was found with P. falciparum. With these reconfirmed P. vivax samples the genotyping was begun, although only in 96.4% (53/55) was the fragment of MSP3 amplified and analyzed by PCR-RFLP using the enzymes Hha I and Alu I to obtain restriction patterns. It was found that amplified fragments of MSP3α gene in 100% (53/53) showed sizes of 1500 bp. Digestion with Hha I and Alu I each yielded a single pattern, and band sizes were similar in all samples. In 49.1% (26/53) of patients a high parasite density (1,485 to 20,225 parasites per µl of blood) and anemia were found, both in 53.8% (14/26) of them. It is concluded that among patients with P. vivax malaria from Bellavista District a single genotype of MSP3 gene is circulating, and it is associated with a high parasite density and anemia.