Mecanismo de acción del infuso de gentianella gilgiana para disminuir la hiperglicemia en rattus norvegicus var. albinus diabéticos
Guevara Vásquez, Ana María Del Carmen
MetadatosMostrar el registro completo del ítem
The effect of Gentianella gilgiana infuse into the intestinal absorption of glucose in vivo and in pancreatic function of Rattus norvegicus var. albinus was studied to determine the mechanism by which this plant exerts its hypoglycemic effect. A total of 32 male specimens, with average body weight of 200g, 12 hours fasted and water ad libitum, were randomly divided into groups of 16 rats, normoglycemic and hyperglycemic groups (we used alloxan 3%, 150 mg / kg bw vip). Each group had control and problem (plant infuse) subgroups. Portal vein was cannulated to measure glucose absorption from the intestine. For control group blood samples were taken from portal and peripheral blood (tail) in fasting and 30 minutes after food, for the problem group a baseline of glucose was taken, then received infuse 10% (200 mg / kg bw), 15 minutes after received food for measuring postprandial glucose at 30 minutes. Accu-Chek Active glucometer was used to quantify glucose. Postprandial blood glucose increased significantly in the control group (p <0.05) and minimally in the problem group, the histopathology of the pancreas showed extensive damage in the islets of diabetic rats and infused delayed increase in glucose tolerance curve oral glucose (p <0.05). We conclude that Gentianella gilgiana infuse decreases intestinal absorption of glucose in vivo in Rattus norvegicus var albinus with induced diabetes.