Utilización de sanguaza y levadura residual de cervecería sobre la producción de biomasa en saccharomyces cerevisiae irradiada con láser ultravioleta
Saldaña Jiménez, José Antonio
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In the present doctoral thesis, the utility of the industrial waste fish water and residual of brewery yeast (LRC) was evaluated on the production of biomass of Saccharomyses cerevisiae var. ellipsoideus MIT – L51 irradiated with ultraviolet laser (UVA), through speed specifies of growth (µ). After of reactivate the pure culture, this it went under laser irradiation getting as a result an inoculum which was subsequently planted and harvested in Airlift (2.0L) bioreactors. The first , second and third bioreactor samples were worked with S. cerevisiae non - irradiated in grape’s juice supplemented with residual of brewery yeast (LRC) to 100mg/L, 150mg/L and 250mg/L respectively and the fourth, fifth and sixth bioreactor samples with S. cerevisiae irradiated. Similar methology was done to the fish left over waste fish water. During 34 hours the bioprocesses were exposed for three times to the pH: 4,0; 22 + 1ºC; 0,5 vvm. The production of biomass was evaluated through the counting method in the Neubauer camera for viable yeast, being obtained that a 100mg/L the biomass was 8.17E+08 cél/mL y de 0.224 a 200mg/L being these the highest values. However, S. cerevisiae irradiated and cultivated in waste fish water. However, the S. cerevisiae irradiated with laser (UVA) grown in fish water to 0.15 mL/L showed a biomass of 7.98E+08 and 0.174 at 0.10mL/L; so, this amount was higher than the non -irradiated culture In the same habitat or even to the irradiated yeast and grown in LRC. The results show that the S. cerevisiae MIT-L51 is able to obtain and use the nutrients derived from LCR with a great efficiency and effectiveness according to the fish water, That which to great scale, it would contribute significantly to diminish the contamination, besides generating extremely useful products that they would bring economic development at level of the north region of our country. But also the laser irradiation (UVA), generates abnormal dimmers in the genetic material. And this would be decreasing in its . Using the statistical analysis, it was found the existence of significant differences, which was confirmed by the comparison of average test.