Efecto de bioinoculantes bacterianos sobre el crecimiento del cultivo de Zea mays bajo condiciones de invernadero, San Martín, Perú
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente estudio tuvo como propósito evaluar el efecto de la aplicación de bioinoculantes bacterianos sobre el crecimiento de maíz (Zea mays. L.) bajo condiciones de invernadero, en la región San Martín. Se aplicó un diseño experimental (DCA) con 4 tratamientos y 12 repeticiones para cada uno, se consideró como muestra 2 cepas de bacterias (PGPR), obtenidas del banco de cepas del laboratorio de Microbiología Agrícola de la Universidad Nacional de San Martín y 2 Kg de semillas de Zea mays L., maíz amarillo duro hibrido “Atlas 777”. En laboratorio se realizó la reactivación de las cepas bacterianas, identificación por amplificación y secuenciación del gen 16S rDNA; los tratamiento fueros: T1 grupo control, T2 cepa A, T3 cepa B y T4 cepa A+B; en campo, muestras de suelo procedentes del caserío de San Fernando fueron analizadas antes y después de los tratamientos evaluando los parámetros químicos y biológicos colocándose 12 kg en maceteros etiquetados, se sembraron dos semillas de maíz impregnadas con los inoculantes para su germinación; en invernadero se tomaron las medidas morfológicas del maíz (altura de la planta, longitud de la hoja, número de hojas y grosor del tallo). Se consideró la prueba de normalidad y el ANOVA. Se concluye que el tratamiento 4 (cepa A+B) tuvo mayores diferencias significativas en comparación con el grupo control en los parámetros químicos, biológicos y características morfológicas.
The purpose of the present study was to evaluate the effect of the application of bacterial bioinoculants on the growth of corn (Zea mays. L.) under greenhouse conditions, in the San Martín region. An experimental design (DCA) was applied with 4 treatments and 12 repetitions for each one, 2 strains of bacteria (PGPR) were considered as samples, obtained from the strain bank of the Agricultural Microbiology laboratory of the National University of San Martín and 2 Kg of Zea mays L. seeds, hybrid hard yellow corn “Atlas 777”. In the laboratory, the reactivation of the bacterial strains, identification by amplification and sequencing of the 16S rDNA gene, were carried out; The treatments were: T1 control group, T2 strain A, T3 strain B and T4 strain A+B; In the field, soil samples from the San Fernando hamlet were analyzed before and after the treatments, evaluating the chemical and biological parameters, placing 12 kg in labeled pots, two corn seeds impregnated with the inoculants were sown for germination; In the greenhouse, morphological measurements of the corn were taken (plant height, leaf length, number of leaves and stem thickness). The normality test and ANOVA were considered. It is concluded that treatment 4 (strain A+B) had greater significant differences compared to the control group in the chemical, biological parameters and morphological characteristics.
The purpose of the present study was to evaluate the effect of the application of bacterial bioinoculants on the growth of corn (Zea mays. L.) under greenhouse conditions, in the San Martín region. An experimental design (DCA) was applied with 4 treatments and 12 repetitions for each one, 2 strains of bacteria (PGPR) were considered as samples, obtained from the strain bank of the Agricultural Microbiology laboratory of the National University of San Martín and 2 Kg of Zea mays L. seeds, hybrid hard yellow corn “Atlas 777”. In the laboratory, the reactivation of the bacterial strains, identification by amplification and sequencing of the 16S rDNA gene, were carried out; The treatments were: T1 control group, T2 strain A, T3 strain B and T4 strain A+B; In the field, soil samples from the San Fernando hamlet were analyzed before and after the treatments, evaluating the chemical and biological parameters, placing 12 kg in labeled pots, two corn seeds impregnated with the inoculants were sown for germination; In the greenhouse, morphological measurements of the corn were taken (plant height, leaf length, number of leaves and stem thickness). The normality test and ANOVA were considered. It is concluded that treatment 4 (strain A+B) had greater significant differences compared to the control group in the chemical, biological parameters and morphological characteristics.
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