Efecto in vitro del extracto metanólico de las hojas de Momordica charantia (Cucurbitaceae) sobre la embrionación (formación del miracidio) del huevo de Fasciola hepatica
No Thumbnail Available
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
La fascioliasis es una parasitosis de importancia en medicina veterinaria y salud pública. Para el tratamiento se utilizan fasciolicidas sintéticos y su intenso uso ha derivado en menor efectividad debido al establecimiento de poblaciones de Fasciola hepatica resistentes a dichas sustancias. El uso de productos derivados de plantas con actividad biológica ha mostrado ser un método de control promisorio. En este contexto, la presente investigación tuvo el objetivo de determinar el efecto in vitro del extracto metanólico de las hojas de Momordica charantia (Cucurbitaceae) cultivada en Trujillo (Perú), a las concentraciones de 12.5, 25.0 y 50.0 mg/mL, sobre huevos de Fasciola hepatica. Se condujo un experimento in vitro, con cinco repeticiones, para incubar aproximadamente 100 huevos del parásito en 240 uL del extracto en cada una de las concentraciones indicadas y en 240 uL de solución salina al 0.85% (testigo). Los huevos fueron extraídos del útero de adultos del digeneo recolectados del hígado de vacunos infectados naturalmente en la sierra liberteña y el extracto por maceración seguida de concentración utilizando un rotavapor y resuspensión en Tween 80. La incubación se hizo a 20 ±2°C y el efecto se evaluó al décimo segundo día. Se encontró que el extracto metanólico de las hojas de Momordica charantia (Cucurbitaceae) a las concentraciones probadas (12.5, 25.0 y 50.0 mg/mL), impide la embrionación (formación del miracidio) del huevo de Fasciola hepatica in vitro, en porcentajes semejantes: 94.6, 97.2 y 98.6% (p>0.05). Esta es la primera investigación con material botánico peruano y sirve como soporte de investigaciones futuras debido a que sugiere su probable utilidad para el control de infecciones por Fasciola hepatica.
Fascioliasis is a parasitic disease of importance in veterinary medicine and public health. Synthetic fasciolicides are used for treatment and their intense use has resulted in less effectiveness due to the establishment of populations of Fasciola hepatica resistant to these substances. The use of products derived from plants with biological activity has shown to be a promising control method. In this context, the present research had the objective of determining the in vitro effect of the methanolic extract of the leaves of Momordica charantia (Cucurbitaceae) grown in Trujillo (Peru), at concentrations of 12.5, 25.0 and 50.0 mg/mL, on eggs of Fasciola hepatica. An in vitro experiment was conducted, with five repetitions, to incubate approximately 100 parasite eggs in 240 uL of the extract at each of the indicated concentrations and in 240 uL of 0.85% saline solution (control). The eggs were extracted from the uterus of digeneo adults collected from the liver of naturally infected cattle in the Sierra Liberteña and the extract by maceration followed by concentration using a rotary evaporator and resuspension in Tween 80. The incubation was done at 20 ±2°C and the effect was evaluated on the twelfth day. It was found that the methanolic extract of the leaves of Momordica charantia (Cucurbitaceae) at the concentrations tested (12.5, 25.0 and 50.0 mg/mL), prevents embryonation (miracidium formation) of the Fasciola hepatica egg in vitro, in similar percentages: 94.6, 97.2 and 98.6% (p>0.05). This is the first research with Peruvian botanical material and serves as support for future research because it suggests its probable usefulness for the control of Fasciola hepatica infections
Fascioliasis is a parasitic disease of importance in veterinary medicine and public health. Synthetic fasciolicides are used for treatment and their intense use has resulted in less effectiveness due to the establishment of populations of Fasciola hepatica resistant to these substances. The use of products derived from plants with biological activity has shown to be a promising control method. In this context, the present research had the objective of determining the in vitro effect of the methanolic extract of the leaves of Momordica charantia (Cucurbitaceae) grown in Trujillo (Peru), at concentrations of 12.5, 25.0 and 50.0 mg/mL, on eggs of Fasciola hepatica. An in vitro experiment was conducted, with five repetitions, to incubate approximately 100 parasite eggs in 240 uL of the extract at each of the indicated concentrations and in 240 uL of 0.85% saline solution (control). The eggs were extracted from the uterus of digeneo adults collected from the liver of naturally infected cattle in the Sierra Liberteña and the extract by maceration followed by concentration using a rotary evaporator and resuspension in Tween 80. The incubation was done at 20 ±2°C and the effect was evaluated on the twelfth day. It was found that the methanolic extract of the leaves of Momordica charantia (Cucurbitaceae) at the concentrations tested (12.5, 25.0 and 50.0 mg/mL), prevents embryonation (miracidium formation) of the Fasciola hepatica egg in vitro, in similar percentages: 94.6, 97.2 and 98.6% (p>0.05). This is the first research with Peruvian botanical material and serves as support for future research because it suggests its probable usefulness for the control of Fasciola hepatica infections
Description
Keywords
Cucurbitaceae, embrionación, Fasciola hepatica, Momordica charantia