Efecto de una dieta a base de Lupinus mutabilis en el peso y la longitud de Mus musculus (ratón albino) adultos
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Date
2023
Authors
Salinas Morales, Brayan Anderson
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Publisher
Universidad Nacional de Trujillo
Abstract
El objetivo de la investigación fue determinar el efecto de una dieta a base de Lupinus mutabilis “tarwi” sobre el peso y la longitud de Mus musculus Balb/c. Se adquirieron granos de Lupinus mutabilis y secaron en estufa a 100 grados Celsius para luego triturarse hasta reducir su tamaño; se utilizaron 120 ratones destetados Mus musculus BALB/c de tres semanas de edad y se alimentaron con la dieta correspondiente hasta las ocho semanas. Se formaron cinco grupos de experimentación: Lupinus mutabilis con cáscara (GCH-C/C), Lupinus mutabilis sin cáscara (GCH-S/C), Lupinus mutabilis + maíz (GM+CH), maíz (GDM), alimento balanceado estándar (GDE); se tomaron datos del peso, longitud, hemoglobina; los resultados fueron tabulados con una prueba de análisis de varianza (ANOVA) y Tukey, ambas con una confianza de 95%. El mayor peso y longitud fue obtenido por GDE (20,6 ± 1,20 g y 17,78 ± 0,85 cm, respectivamente); la mayor concentración de hemoglobina lo obtuvo GCH-S/C (22,55 ± 3,27 mg/dL) y GCH-C/C (23,54 ± 1,40 mg/dL). Se concluyó que la dieta que obtuvo un mayor efecto sobre el peso y longitud fue GDE; la dieta que presentó el mayor efecto en la concentración de hemoglobina GCH-C/C y GCH-C/S.
The objective of the research was to determine the effect of a diet based on Lupinus mutabilis "tarwi" on the weight and length of Mus musculus Balb/c. Lupinus mutabilis grains were purchased and dried in an oven at 100 degrees Celsius and then crushed until their size was reduced; 100 three-week-old Mus musculus BALB/c weanling mice were used and fed with the corresponding diet until eight weeks. Five experimental groups were formed: Lupinus mutabilis with shell (GCH-C/C), Lupinus mutabilis without shell (GCH-S/C), Lupinus mutabilis + corn (GM+CH), corn (GDM), standard balanced feed (GDE); data of weight, length, hemoglobin were taken; the results were tabulated with an analysis of variance (ANOVA) and Tukey test, both with 95% confidence. The greatest weight and length was obtained by GDE (20,6 ± 1,20 g and 17,78 ± 0,85 cm, respectively); the highest hemoglobin concentration was obtained by GCH-S/C (22,55 ± 3,27 mg/dL) and GCH-C/C (23,54 ± 1,40 mg/dL). It was concluded that the diet that had a greater effect on weight and length was GDE; the diet that presented the greatest effect on the concentration of hemoglobin HCG-C/C and HCG-C/S.
The objective of the research was to determine the effect of a diet based on Lupinus mutabilis "tarwi" on the weight and length of Mus musculus Balb/c. Lupinus mutabilis grains were purchased and dried in an oven at 100 degrees Celsius and then crushed until their size was reduced; 100 three-week-old Mus musculus BALB/c weanling mice were used and fed with the corresponding diet until eight weeks. Five experimental groups were formed: Lupinus mutabilis with shell (GCH-C/C), Lupinus mutabilis without shell (GCH-S/C), Lupinus mutabilis + corn (GM+CH), corn (GDM), standard balanced feed (GDE); data of weight, length, hemoglobin were taken; the results were tabulated with an analysis of variance (ANOVA) and Tukey test, both with 95% confidence. The greatest weight and length was obtained by GDE (20,6 ± 1,20 g and 17,78 ± 0,85 cm, respectively); the highest hemoglobin concentration was obtained by GCH-S/C (22,55 ± 3,27 mg/dL) and GCH-C/C (23,54 ± 1,40 mg/dL). It was concluded that the diet that had a greater effect on weight and length was GDE; the diet that presented the greatest effect on the concentration of hemoglobin HCG-C/C and HCG-C/S.
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Keywords
Lupinus mutabilis, desarrollo corporal, hemoglobina