Resistencia a la meticilina de Staphylococcus aureus aislados de leche de vacas con mastitis de establos del distrito de Paiján, Perú
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Staphylococcus aureus resistente a la meticilina (MRSA) es uno de los principales microorganismos patógenos que se pueden encontrar en la leche representando un grave riesgo para la salud de las personas y a la vez ser un problema económico para las granjas lecheras en todo el mundo ya que causan enfermedades tales como mastitis en el ganado vacuno, lo que conlleva a ser de vital importancia su evaluación ya que es poco estudiado en la región la Libertad. Este estudio tuvo como objetivo conocer cuál es la frecuencia de cultivos de Staphylococcus aureus meticilino resistentes que fueron aislados de muestras de leche de vacas con mastitis de establos del distrito de Paiján. Se obtuvieron diez cultivos de Staphylococcus aureus que fueron aislados a partir de muestras de leche de vacas con mastitis, estos cultivos fueron caracterizados utilizando métodos microbiológicos como la evaluación de la morfología de las colonias en agar sangre, tinción de Gram, actividad de catalasa, producción de coagulasa y crecimiento en agar manitol salado y se mantuvieron en conservación a una temperatura de -20°C en glicerol al 50%. Adicionalmente se continuó con la caracterización bioquímica de estos
S. aureus realizándose pruebas de oxidasa, indol, producción de H2S, Voges-Proskauer y Rojo de metilo. Como prueba confirmatoria de resistencia a la meticilina se empleó la prueba de difusión en agar utilizando los discos de cefoxitina (30 ug) y oxacilina (1 ug). De los 10 aislamientos de Staphylococcus aureus, dos (20%) fueron confirmados como
MRSA.
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogenic microorganisms that can be found in milk, representing a serious risk to human health and at the same time being an economic problem for dairy farms around the world since it They cause diseases such as mastitis in cattle, which makes their evaluation of vital importance since it is little studied in the La Libertad region. This study aimed to know the frequency of cultures of methicillin-resistant Staphylococcus aureus that were isolated from milk samples of cows with mastitis from stables in the district of Paiján. Ten cultures of Staphylococcus aureus were obtained that were isolated from milk samples of cows with mastitis. These cultures were characterized using microbiological methods such as the evaluation of colony morphology on blood agar, Gram stain, catalase activity, production. of coagulase and growth on mannitol salt agar and were kept in conservation at a temperature of -20°C in 50% glycerol. Additionally, the biochemical characterization of these S. aureus continued, performing oxidase, indole, H2S production, Voges-Proskauer and methyl red tests. As a confirmatory test for resistance to methicillin, the agar diffusion test was used using cefoxitin (30 ug) and oxacillin (1 ug) discs. Of the 10 Staphylococcus aureus isolates, two (20%) were confirmed as MRSA.
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogenic microorganisms that can be found in milk, representing a serious risk to human health and at the same time being an economic problem for dairy farms around the world since it They cause diseases such as mastitis in cattle, which makes their evaluation of vital importance since it is little studied in the La Libertad region. This study aimed to know the frequency of cultures of methicillin-resistant Staphylococcus aureus that were isolated from milk samples of cows with mastitis from stables in the district of Paiján. Ten cultures of Staphylococcus aureus were obtained that were isolated from milk samples of cows with mastitis. These cultures were characterized using microbiological methods such as the evaluation of colony morphology on blood agar, Gram stain, catalase activity, production. of coagulase and growth on mannitol salt agar and were kept in conservation at a temperature of -20°C in 50% glycerol. Additionally, the biochemical characterization of these S. aureus continued, performing oxidase, indole, H2S production, Voges-Proskauer and methyl red tests. As a confirmatory test for resistance to methicillin, the agar diffusion test was used using cefoxitin (30 ug) and oxacillin (1 ug) discs. Of the 10 Staphylococcus aureus isolates, two (20%) were confirmed as MRSA.
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Keywords
Resistencia; mastitis bovina; frecuencia; caracterización.