Separación, purificación y efecto analgésico de metabolitos secundarios de los extractos de hojas de Passiflora peduncularis Cav. de La Libertad
No Thumbnail Available
Date
2020
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
Se identificó los metabolitos responsables del efecto analgésico de la Passiflora peduncularis Cav. de La Libertad. La materia vegetal se llevó al laboratorio, una vez acondicionada la muestra, se obtuvo el extracto etanólico de las hojas que posteriormente fue liofilizado, un ejemplar de la planta fue identificada en el Herbario Antenor Orrego (HAO) de la Universidad Privada Antenor Orrego. Se llevó a cabo la purificación de los extractos usando Amberlite® XAD-7HP. Por HPLC Agilent 1100, se encontró 4 picos (A, B, C y D) que corresponde a polifenoles derivados de los ácidos hidroxicinámicos e hidroxibenzoico. Se utilizaron 24 especímenes de Mus musculus var swiss, machos como animales de experimentación. Se trabajó con 4 grupos de ratones, el grupo blanco se le administró solución salina fisiológica por vía intraperitoneal, el grupo control se les administró Tramadol 20 mg/kg por vía intraperitoneal, el grupo problema I se le administró 50 mg/kg del extracto alcohólico, el grupo problema II se le administró 100 mg/kg del extracto alcohólico. Posterior al tratamiento, se evalúo el efecto analgésico en todos los grupos, utilizando el Modelo de Hot Plate, Las medidas de los tiempos del efecto analgésico a los 15 minutos después del tratamiento fueron: 4,913±1,352 seg (blanco), 11,387 ±2,224 seg (patrón), 7,672±2,136 seg (problema I), 8,385±2,260 seg (problema II), a los 30 minutos después del tratamiento fueron: 4,95±1,34 seg (blanco), 12,22±1,49 seg (patrón), 6,70±1,44 seg (problema I), 8,44±3,49 seg (problema II), a los 60 minutos después del tratamiento fueron: 4,785±0,590 seg (blanco), 11,612±2,972 seg (patrón), 9,340±1,337 seg (problema I), 8,947±2,119 seg (problema II). Se observó que el extracto alcohólico a una dosis de 100 mg/Kg muestra mayor efecto analgésico que a la dosis de 50 mg/Kg.
The metabolites responsible for the analgesic effect of the Passiflora peduncularis Cav. of La Libertad were identified. The plant matter was taken to the laboratory, once the sample was conditioned, the ethanolic extract of the leaves was obtained, which was subsequently lyophilized, a copy of the plant was identified in the Antenor Orrego Herbarium (HAO) of the Antenor Orrego Private University. Purification of the extracts was carried out using Amberlite® XAD-7HP. By HPLC Agilent 1100, 4 peaks (A, B, C and D) were found corresponding to polyphenols derived from hydroxycinnamic and hydroxybenzoic acids. 24 specimens of Mus musculus var swiss, males were used as experimental animals. Worked with 4 groups of mice, the white group was administered physiological saline intraperitoneally, the control group was administered Tramadol 20 mg / kg intraperitoneally, problem group I was administered 50 mg / kg of the alcoholic extract, problem group II was administered 100 mg / kg of the alcoholic extract. After treatment, the analgesic effect was evaluated in all groups, using the Hot Plate Model. The measures of the analgesic effect times at 15 minutes after treatment were: 4,913 ± 1,352 sec (white), 11,387 ± 2,224 sec (pattern), 7,672 ± 2,136 sec (problem I), 8,385 ± 2,260 sec (problem II), at 30 minutes after treatment were: 4.95 ± 1.34 sec (white), 12.22 ± 1.49 sec (pattern), 6.70 ± 1.44 sec (problem I), 8.44 ± 3.49 sec (problem II), at 60 minutes after treatment were: 4.785 ± 0.590 sec (white), 11.612 ± 2,972 sec (pattern), 9,340 ± 1,337 sec (problem I), 8,947 ± 2,119 sec (problem II). It was observed that the alcoholic extract at a dose of 100 mg / kg shows greater analgesic effect than at the dose of 50 mg / kg.
The metabolites responsible for the analgesic effect of the Passiflora peduncularis Cav. of La Libertad were identified. The plant matter was taken to the laboratory, once the sample was conditioned, the ethanolic extract of the leaves was obtained, which was subsequently lyophilized, a copy of the plant was identified in the Antenor Orrego Herbarium (HAO) of the Antenor Orrego Private University. Purification of the extracts was carried out using Amberlite® XAD-7HP. By HPLC Agilent 1100, 4 peaks (A, B, C and D) were found corresponding to polyphenols derived from hydroxycinnamic and hydroxybenzoic acids. 24 specimens of Mus musculus var swiss, males were used as experimental animals. Worked with 4 groups of mice, the white group was administered physiological saline intraperitoneally, the control group was administered Tramadol 20 mg / kg intraperitoneally, problem group I was administered 50 mg / kg of the alcoholic extract, problem group II was administered 100 mg / kg of the alcoholic extract. After treatment, the analgesic effect was evaluated in all groups, using the Hot Plate Model. The measures of the analgesic effect times at 15 minutes after treatment were: 4,913 ± 1,352 sec (white), 11,387 ± 2,224 sec (pattern), 7,672 ± 2,136 sec (problem I), 8,385 ± 2,260 sec (problem II), at 30 minutes after treatment were: 4.95 ± 1.34 sec (white), 12.22 ± 1.49 sec (pattern), 6.70 ± 1.44 sec (problem I), 8.44 ± 3.49 sec (problem II), at 60 minutes after treatment were: 4.785 ± 0.590 sec (white), 11.612 ± 2,972 sec (pattern), 9,340 ± 1,337 sec (problem I), 8,947 ± 2,119 sec (problem II). It was observed that the alcoholic extract at a dose of 100 mg / kg shows greater analgesic effect than at the dose of 50 mg / kg.
Description
Keywords
Passiflora peduncularis Cav., Efecto analgésico, Mus musculus var swiss