Efecto del liofilizado de hojas de Mangífera indica L “Mango Kent” sobre la lipoperoxidación inducida en mucosa gástrica de Rattus novergicus Sprague-Dawley ovariectomizadas
No Thumbnail Available
Date
2020
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
La finalidad de esta investigación fue determinar el efecto que posee el extracto hidroalcohólico liofilizado de las hojas de Mangifera indica L “Mango kent” sobre la lipoperoxidación inducida en la mucosa gástrica de ratas ovariectomizadas. Se dispuso de 24 ratas de la especie Rattus rattus cepa Sprague-Dawley a las cuales se las distribuyó en 4 grupos. Todas las ratas, excepto las del grupo blanco fueron ovariectomizadas previamente. El grupo blanco no tuvo tratamiento alguno sólo sirvió de referencia, grupo control se le administró vía oral 1 ml/kg p.c de solución de NaCl 0.9g%, los grupos problema 1 y 2 recibieron dosis de 0,25 g/kg y 0.5 g/kg p.c del extracto liofilizado de hojas de Mangifera indica. Las sustancias descritas fueron administradas durante un periodo de 28 días, pasados éstos, se procedió a sacrificar a los especímenes, se realizó el preparado de los homogenizados de los estómagos, luego se añadió ácido tiobarbitútico (0,8%) (TBA) para finalmente hacer las lecturas en espectrofotómetro a 532 nm y realizar los cálculos para hallar la concentración del Malondiadehido (MDA). Los niveles promedio de MDA (nmol/ml): 1,48±0,64 (Blanco), 2,18±0,73 (Control), 1,88±0,82 (Problema 1), 2,64±1,24 (Problema 2). El análisis estadístico se realizó con ANOVA, prueba Duncan y Tukey con un nivel de significancia p<0,05, este análisis reveló que no existe diferencia estadísticamente significativa entre los grupos de experimentación. De acuerdo a los datos obtenidos y análisis realizados se concluye que el extracto liofilizado de Mangífera indica L, en dosis de 0.25 g/kg p.c. reduce los niveles de MDA de manera no estadísticamente significativa (p≥0.05); asimismo en dosis de 0.5g/kg p.c. produjo un aumento de los niveles de MDA en mucosa gástrica de ratas ovariectomizadas
The purpose of this investigation was to determine the effect that has the lyophilized hydroalcoholic extract of the leaves of Mangifera indicates L "Mango kent" on the lipoperoxidation induced in the gastric mucosa of ovariectomized rats. There were 24 rats of the species Rattus rattus strain Sprague-Dawley to which is distributed in 4 groups. All rats, except those in the target group, were previously ovariectomized. The white group does not have adequate treatment. The control group was orally administered 1 ml / kg bw of 0.9 g% NaCl solution, problem groups 1 and 2 received a dose of 0.25 g / kg and 0.5 g / kg bw of the lyophilized extract of leaves of Mangifera indica. The described substances were administered during a period of 28 days, afterwards, the specimens were sacrificed, the preparation of the homogenates of the stomachs was prepared, then thiobarbitimetric acid (0.8%) was added (TBA) to finally make the readings in a spectrophotometer at 532 nm and perform the calculations to find the concentration of Malondiadehyde (MDA). The average levels of MDA (nmol / ml): 1.48 ± 0.64 (White), 2.18 ± 0.73 (Control), 1.88 ± 0.82 (Problem 1), 2.64 ± 1 , 24 (Problem 2). The statistical analysis was performed with ANOVA, Duncan and Tukey test with a level of significance p <0.05, this analysis revealed that there is no statistically significant difference between the experimental groups. According to the data obtained and the analysis carried out, it is concluded that the lyophilized extract of Mangífera L, in doses of 0.25 g / kg b.w. reduce MDA levels in a non-statistically significant way (p≥0.05); also in doses of 0.5g / kg b.w. produced an increase in MDA levels in the gastric mucosa of ovariectomized rats.
The purpose of this investigation was to determine the effect that has the lyophilized hydroalcoholic extract of the leaves of Mangifera indicates L "Mango kent" on the lipoperoxidation induced in the gastric mucosa of ovariectomized rats. There were 24 rats of the species Rattus rattus strain Sprague-Dawley to which is distributed in 4 groups. All rats, except those in the target group, were previously ovariectomized. The white group does not have adequate treatment. The control group was orally administered 1 ml / kg bw of 0.9 g% NaCl solution, problem groups 1 and 2 received a dose of 0.25 g / kg and 0.5 g / kg bw of the lyophilized extract of leaves of Mangifera indica. The described substances were administered during a period of 28 days, afterwards, the specimens were sacrificed, the preparation of the homogenates of the stomachs was prepared, then thiobarbitimetric acid (0.8%) was added (TBA) to finally make the readings in a spectrophotometer at 532 nm and perform the calculations to find the concentration of Malondiadehyde (MDA). The average levels of MDA (nmol / ml): 1.48 ± 0.64 (White), 2.18 ± 0.73 (Control), 1.88 ± 0.82 (Problem 1), 2.64 ± 1 , 24 (Problem 2). The statistical analysis was performed with ANOVA, Duncan and Tukey test with a level of significance p <0.05, this analysis revealed that there is no statistically significant difference between the experimental groups. According to the data obtained and the analysis carried out, it is concluded that the lyophilized extract of Mangífera L, in doses of 0.25 g / kg b.w. reduce MDA levels in a non-statistically significant way (p≥0.05); also in doses of 0.5g / kg b.w. produced an increase in MDA levels in the gastric mucosa of ovariectomized rats.
Description
Keywords
Antioxidante. Mangífera índica L “mango Kent”. Mucosa gástrica. Estrógenos. Malondialdehido (MDA)