Efecto antibacteriano In Vitro del extracto etanólico de hojas de sambucus peruviana "Sauco" sobre streptococcus mutans ATCC 25175
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Date
2018
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Universidad Nacional de Trujillo
Abstract
OBJETIVO: Evaluar el efecto antibacteriano In vitro del extracto etanólico de hojas de Sambucus peruviana sobre Streptococcus mutans ATCC 25175.
MATERIAL Y MÉTODO: Se realizó el extracto etanólico de hojas de Sambucus peruviana por maceración, obteniéndose tres concentraciones. El efecto antibacteriano se determinó mediante el método de Kirby y Bauer, midiendo el diámetro del halo de inhibición. Asimismo se determinó la concentración mínima inhibitoria (CMI) mediante el método de cuentas viables según Unidades formadoras de colonias. En ambos casos se utilizaron concentraciones de 25, 50 y 75%, además de un fármaco control (Penicilina procaínica).
RESULTADOS: Según el método de Kirby y Bauer, el diámetro promedio del halo de inhibición fue: 6.1 mm, 8.9 mm y 15.8 mm para las concentraciones de 25, 50 y 75% respectivamente. El grupo control con penicilina tuvo mayor efecto que las 3 concentraciones del extracto de hojas de sauco (Diámetro de 43 mm). Según el método de cuentas viables mediante las UFC, el resultado obtenido fue: 8.2, 5 y 2.5 para las concentraciones de 25, 50 y 75% respectivamente. La CMI del extracto etanólico de hojas de Sambucus peruviana fue de 25%. El grupo control con penicilina presentó efecto bactericida. CONCLUSIÓN: El extracto etanólico de hojas de Sambucus peruviana “Sauco” presenta efecto antibacteriano “In vitro” sobre Streptococcus mutans ATCC 25175
OBJECTIVE: To evaluate the in vitro antibacterial effect of the ethanolic extract of Sambucus peruviana leaves on Streptococcus mutans ATCC 25175. MATERIAL AND METHOD: The ethanolic extract of Sambucus peruviana leaves was made by maceration, obtaining three concentrations. The antibacterial effect was determined by the Kirby and Bauer method, measuring the diameter of the inhibition halo. Likewise, the minimum inhibitory concentration (MIC) was determined by the method of viable accounts according to colony forming units. In both cases concentrations of 25, 50 and 75% were used, in addition to a control drug (Penicillin procaine). RESULTS: According to the method of Kirby and Bauer, the average diameter of the inhibition halo was: 6.1 mm, 8.9 mm and 15.8 mm for the concentrations of 25, 50 and 75% respectively. The control group with penicillin had greater effect than the 3 concentrations of the elder leaf extract (Diameter of 43 mm). According to the method of viable accounts through the CFU, the result obtained was: 8.2, 5 and 2.5 for the concentrations of 25, 50 and 75% respectively. The MIC of the ethanolic extract of Sambucus peruviana leaves was 25%. The control group with penicillin had a bactericidal effect. CONCLUSION: The ethanolic extract of Sambucus peruviana leaves "Sauco" has an antibacterial effect "In vitro" on Streptococcus mutans ATCC 25175
OBJECTIVE: To evaluate the in vitro antibacterial effect of the ethanolic extract of Sambucus peruviana leaves on Streptococcus mutans ATCC 25175. MATERIAL AND METHOD: The ethanolic extract of Sambucus peruviana leaves was made by maceration, obtaining three concentrations. The antibacterial effect was determined by the Kirby and Bauer method, measuring the diameter of the inhibition halo. Likewise, the minimum inhibitory concentration (MIC) was determined by the method of viable accounts according to colony forming units. In both cases concentrations of 25, 50 and 75% were used, in addition to a control drug (Penicillin procaine). RESULTS: According to the method of Kirby and Bauer, the average diameter of the inhibition halo was: 6.1 mm, 8.9 mm and 15.8 mm for the concentrations of 25, 50 and 75% respectively. The control group with penicillin had greater effect than the 3 concentrations of the elder leaf extract (Diameter of 43 mm). According to the method of viable accounts through the CFU, the result obtained was: 8.2, 5 and 2.5 for the concentrations of 25, 50 and 75% respectively. The MIC of the ethanolic extract of Sambucus peruviana leaves was 25%. The control group with penicillin had a bactericidal effect. CONCLUSION: The ethanolic extract of Sambucus peruviana leaves "Sauco" has an antibacterial effect "In vitro" on Streptococcus mutans ATCC 25175
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Bacteria, Plantas medicinales