Susceptibilidad antimicrobiana de Escherichia coli aislada de pacientes con infección del tracto urinario atendidos en el Centro de Salud Contumazá – Cajamarca – 2021
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Date
2023
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Universidad Nacional de Trujillo
Abstract
En esta investigación se determinó la susceptibilidad antimicrobiana de Escherichia coli
aislada de pacientes con infección del tracto urinario atendidos en el Centro de Salud
Contumazá, Cajamarca, de enero a julio del 2021. Las muestras de orina se inocularon en
Agar Sangre (AS) y Agar Mac Conkey (AMC), después fueron incubados a 37°C durante
24 a 48 horas. Posterior a ello, a partir de las muestras de orina se seleccionaron las colonias
con características macroscópicas para E. coli; con recuento superior a 105 UFC/mL en Agar
Mac Conkey (AMC). Se identificó a E. coli bioquímicamente, mediante las siguientes
pruebas bioquímicas: ureasa, citrato, fermentación de glucosa, lactosa y/o sacarosa en medio
TSI, descarboxilación de lisina en medio LIA, producción de ácido sulfhídrico e indol,
prueba de movilidad en tubos con medio SIM y la prueba de VP-RM. Las colonias aisladas
e identificadas como E. coli se inocularon en Agar Soya Tripticasa (TSA) y se incubaron a
37°C por 24 h para obtener cultivos puros. Luego, se preparó el inoculo semejante al tubo
Nº 0,5 de la escala de Mac Farland (1.5 x 108 UFC/mL), que fue sembrado en placas con
agar Müeller Hinton. A fin de establecer la susceptibilidad antimicrobiana, mediante el
método de discos de difusión en placa, para la evaluación se hizo uso de 10 antibióticos:
Amikacina, Ceftazidima, Ceftriaxona, Amoxicilina/Ac. Clávulamico, Nitrofurantoina,
Cefalexina, Gentamicina, Ciprofloxacino, Norfloxanina y Ácido Nalidixico. Los resultados
más resaltantes de susceptibilidad antimicrobiana de E.coli se presentaron frente a
Amikacina (90%), Ceftazidima (88%), Ceftriaxona (86%), Amoxicilina/Ac. Clávulamico
(82%), Nitrofurantoina (80%), y Cefalexina (75%) y de mayor resistencia correspondieron
a Ácido nalidixico (56%), Norfloxanina (54%), Ciprofloxacino (44%) y Gentamicina (36%).
n this investigation, the antimicrobial susceptibility of Escherichia coli isolated from patients with urinary tract infection treated at the Contumazá Health Center, Cajamarca, from January to July 2021, was determined. Urine samples were inoculated on Blood Agar (AS) and Agar Mac Conkey (AMC), then they were incubated at 37°C for 24 to 48 hours. Subsequently, colonies with macroscopic characteristics for E. coli were selected from the urine samples; with a count greater than 105 CFU/Ml on Mac Conkey Agar (AMC). E. coli was identified biochemically, through the following biochemical tests: urease, citrate, glucose, lactose and/or sucrose fermentation in TSI medium, lysine decarboxylation in LIA medium, hydrogen sulfide and indole production, mobility test in tubes with half a SIM and the VP-RM test. The isolated colonies identified as E. coli were inoculated on Trypticase Soy Agar (TSA) and incubated at 37°C for 24 h to obtain pure cultures. Then, the inoculum similar to tube No. 0.5 of the Mac Farland scale (1.5 x 108 CFU/mL) was prepared, which was seeded on Müeller Hinton agar plates. In order to establish antimicrobial susceptibility, using the plate diffusion disc method. For the evaluation, 10 antibiotics were used: Amikacin, Ceftazidime, Ceftriaxone, Amoxicillin/Ac. Clavulamic, Nitrofurantoin, Cephalexin, Gentamicin, Ciprofloxacin, Norfloxanin and Nalidixic Acid. The most outstanding results of antimicrobial susceptibility of E. coli were presented against Amikacin (90%), Ceftazidime (88%), Ceftriaxone (86%), Amoxicillin/Ac. Clavulamic (82%), Nitrofurantoin (80%), and Cephalexin (75%) and the highest resistance corresponded to Nalidixic acid (56%), Norfloxanine (54%), Ciprofloxacin (44%) and Gentamicin (36%).
n this investigation, the antimicrobial susceptibility of Escherichia coli isolated from patients with urinary tract infection treated at the Contumazá Health Center, Cajamarca, from January to July 2021, was determined. Urine samples were inoculated on Blood Agar (AS) and Agar Mac Conkey (AMC), then they were incubated at 37°C for 24 to 48 hours. Subsequently, colonies with macroscopic characteristics for E. coli were selected from the urine samples; with a count greater than 105 CFU/Ml on Mac Conkey Agar (AMC). E. coli was identified biochemically, through the following biochemical tests: urease, citrate, glucose, lactose and/or sucrose fermentation in TSI medium, lysine decarboxylation in LIA medium, hydrogen sulfide and indole production, mobility test in tubes with half a SIM and the VP-RM test. The isolated colonies identified as E. coli were inoculated on Trypticase Soy Agar (TSA) and incubated at 37°C for 24 h to obtain pure cultures. Then, the inoculum similar to tube No. 0.5 of the Mac Farland scale (1.5 x 108 CFU/mL) was prepared, which was seeded on Müeller Hinton agar plates. In order to establish antimicrobial susceptibility, using the plate diffusion disc method. For the evaluation, 10 antibiotics were used: Amikacin, Ceftazidime, Ceftriaxone, Amoxicillin/Ac. Clavulamic, Nitrofurantoin, Cephalexin, Gentamicin, Ciprofloxacin, Norfloxanin and Nalidixic Acid. The most outstanding results of antimicrobial susceptibility of E. coli were presented against Amikacin (90%), Ceftazidime (88%), Ceftriaxone (86%), Amoxicillin/Ac. Clavulamic (82%), Nitrofurantoin (80%), and Cephalexin (75%) and the highest resistance corresponded to Nalidixic acid (56%), Norfloxanine (54%), Ciprofloxacin (44%) and Gentamicin (36%).
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Keywords
Escherichia coli, Urocultivos, Susceptibilidad