Determinación de la comunidad bacteriana de la cuenca baja del río Moche, mediante análisis metagenómicos 2020
No Thumbnail Available
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
La presente tesis tuvo como objetivo general determinar la comunidad bacteriana en la
cuenca baja del río Moche mediante análisis metagenómicos y como objetivos específicos
determinar los parámetros fisicoquímicos así como evaluar la biodiversidad alfa y beta de la
microbiota bacteriana. Se evaluó en periodos de estiaje, avenida y transición. Los parámetros
analizados fueron pH, temperatura, oxígeno disuelto, demanda bioquímica de oxígeno, entre
otros. Estos análisis tuvieron como referente los Estándares de Calidad Ambiental para el
Agua en la categoría 3 según el MINAM 2017, siendo el pH, oxígeno disuelto y demanda
bioquímica de oxígeno los que se alejaban de sus valores normales. Para conocer como
estaba conformada la comunidad bacteriana se realizó la extracción de ADN, luego la
secuenciación del gen 16S rRNA en la región V3-V4 y finalizó con el análisis
bioinformáticos que reportó la presencia de 24 filos, 19 clases, 24 órdenes, 23 familias y 22
géneros. Así mismo se verificó la biodiversidad Alfa y Beta, la cual indicó a este espacio
como muy diverso y de poca similitud. Los más representativos fueron el filo Proteobacteria,
Bacteroidota, Actinobacteroidota; el orden Burkholderiales, la clase Gammaproteobacteria,
la familia Gallionellaceae y el género Gallionella.
The present thesis had the general objective of determining the bacterial community in the lower basin of the Moche River through metagenomics analyses and as specific objectives to assess physicochemical parameters and evaluate the alpha and beta biodiversity of the bacterial microbiota. The river was evaluated during drought, flood, and transition periods. The parameters analyzed were pH, temperature, dissolved oxygen, biochemical oxygen demand, and others. These analyses were based on the Environmental Quality Standards for Water in category three, according to MINAM 2017, and pH, dissolved oxygen, and biochemical oxygen demand deviated from their average values. To determine the composition of the bacterial community, DNA extraction was performed, followed by sequencing of the 16S rRNA gene in the V3-V4 region, and ending with bioinformatics analyses that reported the presence of 24 phyla, 19 classes, 24 orders, 23 families, and 22 genera. It also verified that Alpha and Beta diversity indicated this space was very diverse and had little similarity. The most representative were the phylum Proteobacteria, Bacteroidetes, Actinobacteria, the order Burkholderiales, the class Gammaproteobacteria, the family Gallionellaceae, and the genus Gallionella.
The present thesis had the general objective of determining the bacterial community in the lower basin of the Moche River through metagenomics analyses and as specific objectives to assess physicochemical parameters and evaluate the alpha and beta biodiversity of the bacterial microbiota. The river was evaluated during drought, flood, and transition periods. The parameters analyzed were pH, temperature, dissolved oxygen, biochemical oxygen demand, and others. These analyses were based on the Environmental Quality Standards for Water in category three, according to MINAM 2017, and pH, dissolved oxygen, and biochemical oxygen demand deviated from their average values. To determine the composition of the bacterial community, DNA extraction was performed, followed by sequencing of the 16S rRNA gene in the V3-V4 region, and ending with bioinformatics analyses that reported the presence of 24 phyla, 19 classes, 24 orders, 23 families, and 22 genera. It also verified that Alpha and Beta diversity indicated this space was very diverse and had little similarity. The most representative were the phylum Proteobacteria, Bacteroidetes, Actinobacteria, the order Burkholderiales, the class Gammaproteobacteria, the family Gallionellaceae, and the genus Gallionella.
Description
Keywords
HUMANITIES and RELIGION::History and philosophy subjects::History subjects::History