Determinación del ratio de biodegradación de hidrocarburos totales de petróleo empleando Pseudomonas sp. en agua de mar contaminada con diésel-B5
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Date
2024
Authors
Peña Ventura, Juan Carlos
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Universidad Nacional de Trujillo
Abstract
El presente trabajo se enfoca en la determinación del ratio de biodegradación de hidrocarburos totales de petróleo, para el cual se realizó una toma de muestra de agua de mar del balneario de Huanchaco - Trujillo y se contaminó con diésel-B5, posterior a ello se empleó Pseudomonas sp. adquirida de un laboratorio certificado a fin de tener el control más idóneo en el conteo y posterior biodegradación, este estudio será de mucha ayuda para nuestra sociedad que viene impactando negativamente en el ecosistema marino contaminado con agua de sentina y múltiples derrames de hidrocarburos que afectan nuestra biodiversidad. La prueba se aplicó a las concentraciones de 10%, 15% y 20% y a temperaturas de 25 °C, 35 °C y 45 °C en un laboratorio microbiológico, teniendo como resultado un mejor ratio de biodegradación por Pseudomonas sp. de 70.9%. Concluyendo que se alcanza la mejor tasa de biodegradación a la temperatura de 35 °C siendo la concentración del 20% de hidrocarburo.
The present work focuses on the determination of the biodegradation ratio of total petroleum hydrocarbons, for which a seawater sample was taken from the watering place Huanchaco - Trujillo and contaminated with diésel-B5, after which we used Pseudomonas sp., said bacterium was acquired by a certified laboratory in order to have the most optimal control in the count and subsequent biodegradation, this degradation will be very helpful for our society that has been contaminated with bilge water and multiple hydrocarbon spills that our biodiversity. The test was applied at concentrations of 10%, 15% and 20% and at temperatures of 25 °C, 35 °C and 45 °C in a microbiological laboratory, resulting in a better rate of biodegradation by Pseudomonas sp. of 70.9%. Concluding that a better biodegradation ratio is achieved at a temperature of 35°C and with a concentration of 20%.
The present work focuses on the determination of the biodegradation ratio of total petroleum hydrocarbons, for which a seawater sample was taken from the watering place Huanchaco - Trujillo and contaminated with diésel-B5, after which we used Pseudomonas sp., said bacterium was acquired by a certified laboratory in order to have the most optimal control in the count and subsequent biodegradation, this degradation will be very helpful for our society that has been contaminated with bilge water and multiple hydrocarbon spills that our biodiversity. The test was applied at concentrations of 10%, 15% and 20% and at temperatures of 25 °C, 35 °C and 45 °C in a microbiological laboratory, resulting in a better rate of biodegradation by Pseudomonas sp. of 70.9%. Concluding that a better biodegradation ratio is achieved at a temperature of 35°C and with a concentration of 20%.
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