Actividad antibacteriana del extracto hidroetanólico de Smallanthus glabratus (DC.) H. Rob. frente a Bacillus subtilis y Pseudomonas alcaliphila
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente estudio tuvo como objetivo evaluar la actividad antibacteriana del extracto hidroalcohólico de las hojas de Smallanthus glabratus (DC.) H. Rob. frente a Bacillus subtilis y Pseudomonas alcaliphila. A partir de las hojas, recolectadas en Otuzco – La Libertad, se preparó el extracto con etanol al 50%. Luego, se evaluó la actividad antibacteriana mediante el método de difusión en disco y microdilución frente a Bacillus subtilis y Pseudomonas alcaliphila. En la difusión por disco se utilizaron cinco cantidades (5 mg; 2,5 mg; 1mg; 0,5 mg y 0,25 mg) del extracto, obteniendo halos de inhibición entre 7 a 8 mm (p<0.05) y 10,7 a 25,3 mm (p<0,05) frente a B. subtilis y P. alcaliphila, respectivamente. Se encontró concentraciones mínimas inhibitorias (CMI) de 0,0146 mg/mL (Bacillus subtilis) y 0,012 mg/mL (Pseudomonas alcaliphila) y concentraciones mínimas bactericidas (CMB) de 0,0586 μg/μL y 0,023 μg/μL para Bacillus subtilis y Pseudomonas alcaliphila, respectivamente. A partir de estos hallazgos, se determinó que el extracto presentó actividad bacteriostática, frente a Bacillus subtilis y bactericida, frente a Pseudomona alcaliphila. Se concluye que las hojas de S. glabratus (DC.) H. Rob. presentan actividad antibacteriana, pudiendo constituir una alternativa en la medicina tradicional de Otuzco
ABSTRACT The objective of the present study was to evaluate the antibacterial activity of the hydroalcoholic extract of the leaves of Smallanthus glabratus (DC.) H. Rob. against Bacillus subtilis and Pseudomonas alcaliphila. From the leaves, collected in Otuzco – La Libertad, the extract was prepared with 50% ethanol. Then, the antibacterial activity was evaluated by the disk diffusion and microdilution method against Bacillus subtilis and Pseudomonas alcaliphila. In disk diffusion, five quantities (5 mg; 2,5 mg; 1mg; 0,5 mg y 0,25 mg) of the extract were used, obtaining inhibition zones between 7 to 8 mm (p < 0,05) and 10,7 to 25, 3 mm (p<0,05) against B. subtilis and P. alcaliphila, respectively. Minimum inhibitory concentrations (MIC) of 0,0146 mg/mL (Bacillus subtilis) and 0,012 mg/mL (Pseudomonas alcaliphila) and minimum bactericidal concentrations (MBC) of 0,0586 μg/μL and 0,023 μg/L were found. μL for Bacillus subtilis and Pseudomonas alcaliphila, respectively. From these findings, it was determined that the extract presented bacteriostatic activity against Bacillus subtilis and bactericidal activity against Pseudomonas alcaliphila. It is concluded that the leaves of S. glabratus (DC.) H. Rob. They present antibacterial activity, and may constitute an alternative in the traditional medicine of Otuzco.
ABSTRACT The objective of the present study was to evaluate the antibacterial activity of the hydroalcoholic extract of the leaves of Smallanthus glabratus (DC.) H. Rob. against Bacillus subtilis and Pseudomonas alcaliphila. From the leaves, collected in Otuzco – La Libertad, the extract was prepared with 50% ethanol. Then, the antibacterial activity was evaluated by the disk diffusion and microdilution method against Bacillus subtilis and Pseudomonas alcaliphila. In disk diffusion, five quantities (5 mg; 2,5 mg; 1mg; 0,5 mg y 0,25 mg) of the extract were used, obtaining inhibition zones between 7 to 8 mm (p < 0,05) and 10,7 to 25, 3 mm (p<0,05) against B. subtilis and P. alcaliphila, respectively. Minimum inhibitory concentrations (MIC) of 0,0146 mg/mL (Bacillus subtilis) and 0,012 mg/mL (Pseudomonas alcaliphila) and minimum bactericidal concentrations (MBC) of 0,0586 μg/μL and 0,023 μg/L were found. μL for Bacillus subtilis and Pseudomonas alcaliphila, respectively. From these findings, it was determined that the extract presented bacteriostatic activity against Bacillus subtilis and bactericidal activity against Pseudomonas alcaliphila. It is concluded that the leaves of S. glabratus (DC.) H. Rob. They present antibacterial activity, and may constitute an alternative in the traditional medicine of Otuzco.
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Keywords
Asteraceae. Smallanthus glabratus. Actividad bactericida. Actividad bacteriostática