Efecto de siete concentraciones de ácido indol butírico en el enraizamiento de vitro plantas de vaccinium corymbosum var. biloxi
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Date
2018
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Universidad Nacional de Trujillo
Abstract
El sistema autotrófico hidropónico (SAH), combina técnicas de micro propagación y de multiplicación autotrófica basada en la capacidad autotrófica de los esquejes y el rol fundamental del microambiente. El objetivo fue determinar el efecto de siete concentraciones de Ácido Indol Butírico en el enraizamiento de vitroplantas de Vaccinium corymbosum var. Biloxi. Se empleó el Diseño Completo al Azar (DCA) con siete tratamientos y tres repeticiones por tratamiento. Los resultados de cada experimento fueron analizados mediante el ANVA al 95% y se compararon las medias de los tratamientos mediante la diferencia mínima significativa en la prueba de Duncan. El Ácido Indol Butírico diluido en alcohol según las concentraciones de cada tratamiento se mezclaron con la solución hidropónica ya preparada. Se colocó 100gr de sustrato en cada una de las bolsas. En un vaso de precipitación se colocó 100 ml de la solución que se obtuvo de la mezcla entre el IBA y la solución hidropónica. Estos 100 ml se agregaron a las bolsas con sustrato, después se procedió a cerrar las bolsas de polipropileno de 3L, utilizando clips. Luego de cerrar las bolsas se procedió a auto clavarlas luego se esperó hasta que el sustrato enfríe. Cuando el sustrato ya estuvo frio se llevaron a la cámara de flujo laminar en la cual se realizó la siembra de las vitro plantas. Se logró obtener plántulas autotróficas de arándano que tienen una gran capacidad de adaptación a las condiciones de invernadero. Se concluyó que la dosis de 60 ppm de IBA incrementó y presentó el mayor número de raíces (8.4 unidades), la mayor longitud de raíces (6.4 cm) y la mayor longitud de raíces (4.4 cm) respecto al control y otras dosis menores de IBA en plántulas in vitro de arándano.
ABSTRACT The system autotrófico hidropónico (SAH), propagation combines techniques of microcomputer and of multiplication autotrófica based in capability autotrófica of the cuttings and the fundamental role of the microambiente. The objective was to determine the effect of seven concentrations of acid Indol Butírico in the enraizamiento of Vaccinium's vitroplantas corymbosum var. Biloxi. You used a Design Complete at random (DCA) with seven treatments and three repetitions for treatment. The results out of every experiment were examined by means of the ANVA to the 95 % and the stockings of the intervening treatments compared the minimal significant difference in Duncan's proof themselves. The Indol Butírico acid diluted in alcohol according to the concentrations out of every treatment they mixed with the solution hidropónica already prepared. 100gr of substratum in each of the bags was placed. 100 ml of the solution that was obtained of the mixture were placed in a glass of precipitation enter the you went and the solution hidropónica. These 100 ml added the bags with substratum themselves, after it was proceeded to closing polipropileno's bags of 3L, utilizing clips. One proceeded to writ to dive next right after closing bags you held on until the substratum cool. When the substratum already was I fry they took the camera of laminar flow in the one that it came true in you set up the vitro's planting. It was managed to obtain plantelets autotróficas of bilberry that have a great capability of adaptation to the conditions of greenhouse. You came to an end than 60 ppm's dose of you went you incremented and dose presented the bigger number of roots (8,4 units ), the bigger length of roots ( 6,4 cm ) and the bigger length of roots ( 4,4 cm ) in relation to control and others younger of was going in plantelets in vitro of bilberry.
ABSTRACT The system autotrófico hidropónico (SAH), propagation combines techniques of microcomputer and of multiplication autotrófica based in capability autotrófica of the cuttings and the fundamental role of the microambiente. The objective was to determine the effect of seven concentrations of acid Indol Butírico in the enraizamiento of Vaccinium's vitroplantas corymbosum var. Biloxi. You used a Design Complete at random (DCA) with seven treatments and three repetitions for treatment. The results out of every experiment were examined by means of the ANVA to the 95 % and the stockings of the intervening treatments compared the minimal significant difference in Duncan's proof themselves. The Indol Butírico acid diluted in alcohol according to the concentrations out of every treatment they mixed with the solution hidropónica already prepared. 100gr of substratum in each of the bags was placed. 100 ml of the solution that was obtained of the mixture were placed in a glass of precipitation enter the you went and the solution hidropónica. These 100 ml added the bags with substratum themselves, after it was proceeded to closing polipropileno's bags of 3L, utilizing clips. One proceeded to writ to dive next right after closing bags you held on until the substratum cool. When the substratum already was I fry they took the camera of laminar flow in the one that it came true in you set up the vitro's planting. It was managed to obtain plantelets autotróficas of bilberry that have a great capability of adaptation to the conditions of greenhouse. You came to an end than 60 ppm's dose of you went you incremented and dose presented the bigger number of roots (8,4 units ), the bigger length of roots ( 6,4 cm ) and the bigger length of roots ( 4,4 cm ) in relation to control and others younger of was going in plantelets in vitro of bilberry.
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Keywords
Enraizamiento, Arándano, In vitro, IBA