Evaluación de la toxicidad aguda de Ambrosia arborescens Mill. sobre parámetros bioquímicos, hematológicos e histopatológicos de Rattus norvegicus var. albinus
No Thumbnail Available
Date
2020
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
Las plantas medicinales desempeñan un papel importante en el manejo de diversas enfermedades por lo que su uso se ha generalizado, pero la población utiliza las especies vegetales sin tener en cuenta la toxicidad que pueden poseer. Esta investigación, se realizó con la finalidad de evaluar la toxicidad aguda del extracto acuoso de las hojas de Ambrosia arborescens Mill. sobre los parámetros bioquímicos, hematológicos e histopatológicos de Rattus norvegicus var. albinus. Material y Métodos: Las hojas de Ambrosia arborescens Mill. fueron colectadas en la provincia de Julcán. Región La Libertad. Se realizó el ensayo Nº 423 de la OECD, formando los siguientes grupos experimentales cada uno conformado por 10 especímenes (5 machos y 5 hembras): Grupo Control, que recibió 2 mL solución salina fisiológica (SSF 0.9%), Grupo Problema I y II, a los que se les administró el extracto acuoso de hojas de Ambrosia arborescens Mill. en dosis de 300 y 2000 mg/Kg/día, respectivamente. El día 14 se realizó la medición de los parámetros bioquímicos (creatinina, GOT, GPT, colesterol y glucosa), hematológicos (serie roja, blanca y plaquetas) e histopatológicos. Resultados: El extracto acuoso de hojas de Ambrosia arborescens Mill. a dosis de 300 mg/kg/día y 2000 mg/kg no afectó a los diversos parámetros hematológicos relacionados a la hematopoyesis y leucopoyesis en ratas, excepto en el caso de las plaquetas cuyo número aumenta significativamente según las dosis, en las ratas hembras. Los parámetros bioquímicos de función renal y hepática estuvieron en los rangos normales, los niveles de colesterol total se reducieron significativamente en ratas machos y hembras a la dosis máxima (2000 mg/kg). En el análisis histopatológico, el tejido hepático y renal evidenciaron congestión leve. Conclusiones: El extracto acuoso de hojas de Ambrosia arborescens Mill. tiene un DL50 mayor de 2000 mg / kg y no evidencia alteraciones significativas en los parámetros bioquímicos, hematológicos e histopatológicos de Rattus novergicus var. albinus.
ABSTRACT Introduction: Medicinal plants play an important role in the management of various diseases, so their use has become widespread, but the population uses plant species without taking into account the toxicity they may have. This investigation was carried out in order to evaluate the acute toxicity of the aqueous extract of the leaves of Ambrosia arborescens Mill. on the biochemical, hematological and histopathological parameters of Rattus norvegicus var. albinus. Material and Methods: The leaves of Ambrosia arborescens Mill. were collected in the Julcán province. La Libertad Region. The test No. 423 - OECD was carried out, forming the following experimental groups each consisting of 10 specimens (5 males and 5 females): Control Group, which received 2 mL physiological saline solution (0.9% SSF), Problem Group I and II, to which the aqueous extract of Ambrosia arborescens Mill leaves was administered in doses of 300 and 2000 mg/Kg/day, respectively. On day 14, the biochemical (creatinine, GOT, GPT, cholesterol and glucose), hematological (red, white and platelet series) and histopathological parameters were measured. Results: The aqueous extract of leaves of Ambrosia arborescens Mill. at doses of 300 mg/kg/day and 2000 mg / kg did not affect the various hematological parameters related to hematopoiesis and leukopoiesis in rats, except in the case of platelets whose number increases significantly according to doses in female rats. The biochemical parameters of kidney and liver function were in the appropriate ranges, total cholesterol levels significantly reduced in male and female rats at the maximum dose (2000 mg / kg). In the histopathological analysis, liver and kidney tissue showed mild congestion. Conclusions: The aqueous extract from the leaves of Ambrosia arborescens Mill. has a LD50 greater than 2000 mg/kg and does not show significant alterations in the biochemical, hematological and histopathological parameters of Rattus novergicus var. albinus.
ABSTRACT Introduction: Medicinal plants play an important role in the management of various diseases, so their use has become widespread, but the population uses plant species without taking into account the toxicity they may have. This investigation was carried out in order to evaluate the acute toxicity of the aqueous extract of the leaves of Ambrosia arborescens Mill. on the biochemical, hematological and histopathological parameters of Rattus norvegicus var. albinus. Material and Methods: The leaves of Ambrosia arborescens Mill. were collected in the Julcán province. La Libertad Region. The test No. 423 - OECD was carried out, forming the following experimental groups each consisting of 10 specimens (5 males and 5 females): Control Group, which received 2 mL physiological saline solution (0.9% SSF), Problem Group I and II, to which the aqueous extract of Ambrosia arborescens Mill leaves was administered in doses of 300 and 2000 mg/Kg/day, respectively. On day 14, the biochemical (creatinine, GOT, GPT, cholesterol and glucose), hematological (red, white and platelet series) and histopathological parameters were measured. Results: The aqueous extract of leaves of Ambrosia arborescens Mill. at doses of 300 mg/kg/day and 2000 mg / kg did not affect the various hematological parameters related to hematopoiesis and leukopoiesis in rats, except in the case of platelets whose number increases significantly according to doses in female rats. The biochemical parameters of kidney and liver function were in the appropriate ranges, total cholesterol levels significantly reduced in male and female rats at the maximum dose (2000 mg / kg). In the histopathological analysis, liver and kidney tissue showed mild congestion. Conclusions: The aqueous extract from the leaves of Ambrosia arborescens Mill. has a LD50 greater than 2000 mg/kg and does not show significant alterations in the biochemical, hematological and histopathological parameters of Rattus novergicus var. albinus.
Description
Keywords
Toxicidad aguda, Ambrosia, extracto acuoso, ratas