Optimización del método analítico de omegas 3 en aceite de pescado para reducir el costo y tiempo de análisis
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El objetivo principal de esta tesis fue optimizar el método analítico de omegas 3, así mismo reducir el costo y tiempo de análisis, se ha tomado muestras de ensayo de 2 analistas cuando se realizó el presente estudio, se usó el software estadístico Minitab, demostrando así, que los residuales de los datos de los analistas se ajustan a una distribución normal, P-value %EPA y %DHA > α = 0.05). La prueba de Grubbs demostró que no se presentan datos atípicos, (estadístico de la prueba G para %EPA y %DHA < valor critico). Se aplicó también la prueba de homogeneidad de varianza F-Test, demostrándose que la variabilidad de los resultados de ensayo entre los analistas es la misma tanto % EPA Y %DHA, (P-value > α = 0.05). Mediante el Análisis de varianza (anova), tanto para %EPA y DHA, se demostró que las medias de los resultados de los analistas son estadísticamente similares, (P-value > α = 0.05). La veracidad de los resultados para %EPA, se ha demostrado aplicando la prueba de T-Student para 2 muestras, concluyendo que los resultados obtenidos en laboratorio de Hayduk son veraces, (P-value > α = 0.05); y para %DHA se aplica el grafico de Z-score, demostrando que los valores absolutos de los resultados de los analistas son < 2, concluyendo que los resultados obtenidos son veraces. Se ha demostrado que los resultados de las muestras de ensayo % EPA y %DHA eliminando la etapa 2 (evaporación de solvente) del método actual son repetibles y reproducibles. Límite de Repetibilidad %EPA, Lr=0.1601, Límite de Repetibilidad %DHA, Lr=0.1700, Límite de Reproducibilidad %EPA, LR= 3.6717, Límite de Reproducibilidad %DHA, LR= 3.7598. El método queda optimizado y validado. Al eliminar la etapa de extracción 2 (evaporación de solvente, se reduce el costo de Análisis de 12.45 dólares a 11.77 dólares, teniendo un ahorro por muestra de 0.68 dólares y en el año un ahorro de costo de 2795 dólares. Igualmente, la reducción de tiempo de Análisis es 0.172 h/muestra y en el año 699 horas.
The main objective of this thesis is to optimize the analytical Method of omegas 3, as well as reduce the cost and time of analysis, for this test samples have been taken from two analysts to carry out the present study. For this study, the Minitab statistical software was used to demonstrate that the residuals of the data from the analysts fit a normal distribution, P-value %EPA and %DHA > α = 0.05). The Grubbs test shows that there are no atypical data that could distort the present research work (G test statistics for % EPA and % DHA < critical value). The F- Test variance homogeneity test was also applied, showing that there is no variability in the test results between the analysts, it is the same for both % EPA and % DHA, (P-value > α = 0.05). Through the analysis of variance (ANOVA), both for % EPA and % DHA, it is shown that the means of the results of the analysts are statistically similar (P-value > α = 0.05). The veracity of the for % EPA has been demonstrated by applying the T-Student test for two samples, concluding that the results obtained in the Hayduk laboratory are true (P-value > α = 0.05). And for % DHA, the Z-score graph is applied, showing that the absolute values of the analysts results are < 2, concluding that the results obtained in Hayduk laboratory are true. The results of the % EPA and % DHA test samples removing step two (solvent evaporation) from the current method have been shown to be repeatable and reproducible. Repeatability Limit %EPA, Lr= 0.1601, Repeatability Limit %DHA, Lr= 0.1700, Reproducibility Limit %EPA, LR= 3.6717, Reproducibility Limit %EPA, LR= 3.7598 The method is optimized and validated. By eliminating extraction stage 2 (solvent evaporation), the cost of Analysis is reduced from 12.45 dollars to 11.77 dollars, having a saving per sample of 0.68 dollars and a cost saving of 2795 dollars in the year.
The main objective of this thesis is to optimize the analytical Method of omegas 3, as well as reduce the cost and time of analysis, for this test samples have been taken from two analysts to carry out the present study. For this study, the Minitab statistical software was used to demonstrate that the residuals of the data from the analysts fit a normal distribution, P-value %EPA and %DHA > α = 0.05). The Grubbs test shows that there are no atypical data that could distort the present research work (G test statistics for % EPA and % DHA < critical value). The F- Test variance homogeneity test was also applied, showing that there is no variability in the test results between the analysts, it is the same for both % EPA and % DHA, (P-value > α = 0.05). Through the analysis of variance (ANOVA), both for % EPA and % DHA, it is shown that the means of the results of the analysts are statistically similar (P-value > α = 0.05). The veracity of the for % EPA has been demonstrated by applying the T-Student test for two samples, concluding that the results obtained in the Hayduk laboratory are true (P-value > α = 0.05). And for % DHA, the Z-score graph is applied, showing that the absolute values of the analysts results are < 2, concluding that the results obtained in Hayduk laboratory are true. The results of the % EPA and % DHA test samples removing step two (solvent evaporation) from the current method have been shown to be repeatable and reproducible. Repeatability Limit %EPA, Lr= 0.1601, Repeatability Limit %DHA, Lr= 0.1700, Reproducibility Limit %EPA, LR= 3.6717, Reproducibility Limit %EPA, LR= 3.7598 The method is optimized and validated. By eliminating extraction stage 2 (solvent evaporation), the cost of Analysis is reduced from 12.45 dollars to 11.77 dollars, having a saving per sample of 0.68 dollars and a cost saving of 2795 dollars in the year.
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