Actividad antihelmíntica in vitro del precipitado proteínico del fruto de Actinidia deliciosa sobre las larvas L2 de Ascaris lumbricoides
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Date
2023
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Universidad Nacional de Trujillo
Abstract
La geohelmintiasis causada por Ascaris lumbricoides (Syn. A. suum) mantiene su vigencia como una de las más prevalentes a nivel mundial y las drogas antihelmínticas sintéticas usadas para su tratamiento tienen efectos colaterales y teratogénicos, por ello, se requiere que otras sustancias constituyan tratamiento alternativo. El objetivo de esta investigación fue determinar la actividad antihelmíntica in vitro del precipitado proteínico (PP) de la pulpa del fruto maduro de Actinidia deliciosa “kiwi” sobre la larva L2 de Ascaris lumbricoides. El PP se obtuvo siguiendo un protocolo validado y las larvas L2 por incubación in vitro de huevos separados directamente de los úteros de hembras recolectadas en cerdos naturalmente infectados y sacrificados en el Camal Municipal de El Porvenir (Trujillo, Perú). La actividad antihelmíntica se probó utilizando 100 larvas L2 recientemente eclosionadas y 200 uL del precipitado completo y diluido al medio; como control se utilizó solución salina estéril (SSFE al 0.8%). El porcentaje de larvas móviles fue de 15.5% y 23.1% en el precipitado completo y diluido, respectivamente. En el grupo control se encontró que el 96.81% de las L2 presentaron movilidad. La observación microscópica de las larvas no móviles reveló lesiones en la cutícula. En conclusión, el precipitado proteínico de Actinidia deliciosa presenta un efecto antihelmíntico sobre las larvas L2 de A. lumbricoides.
Geohelminthiasis caused by Ascaris lumbricoides (Syn. A. suum) maintains its validity as one of the most prevalent worldwide and the synthetic anthelmintic drugs used for its treatment have side and teratogenic effects, therefore, other substances are required to constitute an alternative treatment. The objective of this research was to determine the in vitro anthelmintic activity of the protein precipitate (PP) from the pulp of the ripe fruit of Actinidia deliciosa "kiwi" on the L2 larva of Ascaris lumbricoides. The PP was obtained following a validated protocol and the L2 larvae by in vitro incubation of eggs separated directly from the uteri of females collected from naturally infected and slaughtered pigs in the Municipal Camal of El Porvenir (Trujillo, Peru). The anthelmintic activity was tested using 100 recently hatched L2 larvae and 200 uL of the complete precipitate and diluted in the medium; sterile saline solution (0.8% SSFE) was used as a control. The percentage of mobile larvae was 15.5% and 23.1% in the complete and diluted precipitate, respectively. In the control group it was found that 96.81% of the L2 presented mobility. Microscopic observation of non-motile larvae revealed cuticle lesions. In conclusion, the protein precipitate of Actinidia deliciosa has an anthelmintic effect on L2 larvae of A. lumbricoides.
Geohelminthiasis caused by Ascaris lumbricoides (Syn. A. suum) maintains its validity as one of the most prevalent worldwide and the synthetic anthelmintic drugs used for its treatment have side and teratogenic effects, therefore, other substances are required to constitute an alternative treatment. The objective of this research was to determine the in vitro anthelmintic activity of the protein precipitate (PP) from the pulp of the ripe fruit of Actinidia deliciosa "kiwi" on the L2 larva of Ascaris lumbricoides. The PP was obtained following a validated protocol and the L2 larvae by in vitro incubation of eggs separated directly from the uteri of females collected from naturally infected and slaughtered pigs in the Municipal Camal of El Porvenir (Trujillo, Peru). The anthelmintic activity was tested using 100 recently hatched L2 larvae and 200 uL of the complete precipitate and diluted in the medium; sterile saline solution (0.8% SSFE) was used as a control. The percentage of mobile larvae was 15.5% and 23.1% in the complete and diluted precipitate, respectively. In the control group it was found that 96.81% of the L2 presented mobility. Microscopic observation of non-motile larvae revealed cuticle lesions. In conclusion, the protein precipitate of Actinidia deliciosa has an anthelmintic effect on L2 larvae of A. lumbricoides.
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Keywords
Actinidia deliciosa, Ascaris summ, cistein proteasas, precipitado proteínico, anthelmíntico