Bacillus spp. diazotróficos aislados de la rizósfera de Opuntia quitensis, del Área de Conservación Privada Lomas del Cerro Campana, Trujillo-Perú
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue aislar Bacillus spp. diazotróficos de la rizosfera de Opuntia quitensis “tuna silvestre” del Área de Conservación Privada Lomas del Cerro Campana Trujillo-Perú. Para ello se recolectaron muestras de la rizósfera de 15 ejemplares de O. quitensis. Inicialmente se determinó las bacterias fijadoras de nitrógeno, por medio del método: Número Más Probable (NMP) y de las biopelículas formadas indicando positividad, se sembraron en placas con Agar Nfb, las cuales se incubaron a 30º C durante 3 a 5 días. Las colonias bacterianas aisladas, se identificaron como género Bacillus a través de pruebas fenotípicas y la molecular mediante análisis de la secuencia del gen 16S rRNA B. megaterium, B. nealsonii, B. wiedmannii como las especies aisladas de la tuna silvestre.
The objective of this research was to isolate diazotrophic Bacillus spp. from the rhizosphere of Opuntia quitensis "tuna silvestre'' from the Private Conservation Area Lomas del Cerro Campana Trujillo-Peru. For this purpose, rhizosphere samples were collected from 15 specimens of O. quitensis. Initially, nitrogen-fixing bacteria were determined using the Most Probable Number (MPN) method and the biofilms formed indicating positivity were sown on plates with Nfb Agar, which were incubated at 30º C for 3 to 5 days. The isolated bacterial colonies were identified as belonging to the Bacillus genus through phenotypic tests and molecular analysis using 16S rRNA gene sequence B. megaterium, B. nealsonii, B. wiedmannii as the species isolated from wild prickly pear.
The objective of this research was to isolate diazotrophic Bacillus spp. from the rhizosphere of Opuntia quitensis "tuna silvestre'' from the Private Conservation Area Lomas del Cerro Campana Trujillo-Peru. For this purpose, rhizosphere samples were collected from 15 specimens of O. quitensis. Initially, nitrogen-fixing bacteria were determined using the Most Probable Number (MPN) method and the biofilms formed indicating positivity were sown on plates with Nfb Agar, which were incubated at 30º C for 3 to 5 days. The isolated bacterial colonies were identified as belonging to the Bacillus genus through phenotypic tests and molecular analysis using 16S rRNA gene sequence B. megaterium, B. nealsonii, B. wiedmannii as the species isolated from wild prickly pear.
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Keywords
Bacterias diazotróficas, Bacillus, rizósfera, Opuntia quitensis