Actividad antioxidante del extracto hidroalcohólico de las hojas de Ambrosia arborescens Mill., en leche cruda
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Este trabajo de investigación, tuvo como objetivo, evaluar la actividad antioxidante del extracto hidroalcohólico de las hojas de Ambrosia arborescens Mill., en leche cruda. Se trabajó con cinco grupos: control negativo (GCN), leche cruda sin ningún antioxidante añadido; control positivo (GCP), leche cruda con el antioxidante Butilhidroxitolueno (BHT) a una concentración de 200 mg/kg; experimental 1 (GE1), experimental 2 (GE2) y experimental 3 (GE3) que estuvieron conformados por la leche cruda y el extracto hidroalcohólico de las hojas de A. arborescens a las concentraciones de 250 mg/kg, 500 mg/kg y 1000 mg/kg, respectivamente; en los cinco grupos se indujo la peroxidación lipídica por el método químico Fe3+ - EDTA / ácido ascórbico y luego, se determinó la actividad antioxidante en función a la concentración de malondialdehído (MDA) (producto final de la peroxidación lipídica), mediante el método de las sustancias reactivas al ácido tiobarbitúrico (TBARS); la prueba de ANOVA y Tukey determinaron que existe diferencia estadística (p<0,05) en la actividad antioxidante del extracto hidroalcohólico. Las tres concentraciones del extracto hidroalcohólico de las hojas de Ambrosia arborescens Mill., poseen actividad antioxidante en la leche cruda y, a 1000 mg/kg se logra la mayor actividad antioxidante, pero es menor a la actividad del BHT.
The objective of this research was to evaluate the antioxidant activity of the hydroalcoholic extract of Ambrosia arborescens Mill. leaves in raw milk. Five groups were used: negative control (NCG), raw milk without any antioxidant added; positive control (PCG), raw milk with the antioxidant butylated hydroxytoluene (BHT) at a concentration of 200 mg/kg; experimental 1 (GE1), experimental 2 (GE2) and experimental 3 (GE3), which consisted of raw milk and the hydroalcoholic extract of A. arborescens leaves at concentrations of 250 mg/kg, 500 mg/kg and 1000 mg/kg, respectively; Lipid peroxidation was induced in the five groups by the chemical method Fe3+ - EDTA / ascorbic acid and then, the antioxidant activity was determined according to the concentration of malondialdehyde (MDA) (final product of lipid peroxidation), by the thiobarbituric acid reactive substances (TBARS) method; the ANOVA and Tukey test determined that there was a statistical difference (p<0,05) in the antioxidant activity of the hydroalcoholic extract. The three concentrations of the hydroalcoholic extract of Ambrosia arborescens Mill. leaves have antioxidant activity in raw milk and, at 1000 mg/kg, the highest antioxidant activity is achieved, but it is lower than the activity of BHT.
The objective of this research was to evaluate the antioxidant activity of the hydroalcoholic extract of Ambrosia arborescens Mill. leaves in raw milk. Five groups were used: negative control (NCG), raw milk without any antioxidant added; positive control (PCG), raw milk with the antioxidant butylated hydroxytoluene (BHT) at a concentration of 200 mg/kg; experimental 1 (GE1), experimental 2 (GE2) and experimental 3 (GE3), which consisted of raw milk and the hydroalcoholic extract of A. arborescens leaves at concentrations of 250 mg/kg, 500 mg/kg and 1000 mg/kg, respectively; Lipid peroxidation was induced in the five groups by the chemical method Fe3+ - EDTA / ascorbic acid and then, the antioxidant activity was determined according to the concentration of malondialdehyde (MDA) (final product of lipid peroxidation), by the thiobarbituric acid reactive substances (TBARS) method; the ANOVA and Tukey test determined that there was a statistical difference (p<0,05) in the antioxidant activity of the hydroalcoholic extract. The three concentrations of the hydroalcoholic extract of Ambrosia arborescens Mill. leaves have antioxidant activity in raw milk and, at 1000 mg/kg, the highest antioxidant activity is achieved, but it is lower than the activity of BHT.
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Keywords
Antioxidante, extracto hidroalcohólico, Ambrosia arborescens Mill., leche cruda