Elaboración de una nanoemulsión hidratante a partir de aceite de semillas de Helianthus annuus “girasol” enriquecido con vitamina E
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Esta investigación tuvo el propósito de elaborar una nanoemulsión hidratante con aceite de Helianthus annuus “girasol” enriquecida con vitamina E. Las muestras elaboradas son blanquecinas, sin olor y suaves al tacto. En la elaboración de las nanoemulsiones se utilizó diferentes proporciones del aceite de semillas de girasol, de Tween 80 y agua destilada; pero la cantidad de vitamina E se mantiene constante. Se trabajaron 5 muestras, siendo identificados con los números 1, 2, 3, 4 y 5. Respecto al procedimiento se usó el método de inversión de fases que consistió en verter lentamente la fase acuosa en la fase oleosa en constante agitación a 250 RPM, antes de verter, ambas fases también se mezclan por si solas en constante agitación durante 10 minutos. Según las pruebas cualitativas de estabilidad realizadas a lo largo del tiempo, se encontró que todas las muestras cumplían con los criterios de calidad esperados, pues no se encontraron burbujas de aire, ni sedimentación, ni cremado, todas las muestras mostraron uniformidad en su apariencia, sin partículas insolubles visibles, ni aglomeraciones. Las pruebas de centrifugación y estrés térmico confirmaron que las emulsiones eran estables y no hubo separación de fases durante los 60 días de seguimiento. La viscosidad, durante los 60 días de estudio, en las muestras 1, 2 y 4 se mantuvo entre 4.31 y 4.46 cP; mientras que en las otras muestras 3 y 5 mostraron una viscosidad ligeramente mayor, entre 4.62 y 4.66 cP. El pH de todos los lotes se mantuvo dentro de un rango ligeramente ácido, variando de 6.72 a 6.96 a lo largo del periodo de estudio. Respecto al tamaño de gota, solo las muestras 2, 3, 4 y 5 lograron valores compatibles con las características de una nanoemulsión, con tamaños promedio de 76, 256, 43.5 y 254.3 nm. respectivamente, ajustándose a una distribución gaussiana. En la prueba de estrés térmico todas las muestras fueron estables sin separación de fases durante los 60 días de estudio. En conclusión, las muestras 2 y 4, muestran las características deseadas de estabilidad, tamaño de gota y comportamiento fisicoquímico.
ABSTRACT This research had the purpose of developing a moisturizing nanoemulsion with Helianthus annuus “sunflower” oil enriched with vitamin E. The samples produced are whitish, odorless and soft to the touch. In the preparation of the nanoemulsions, different proportions of sunflower seed oil, Tween 80 and distilled water were used; but the amount of vitamin E remains constant. 5 samples were worked on, being identified with the numbers 1, 2, 3, 4 and 5. Regarding the procedure, the phase inversion method was used, which consisted of slowly pouring the aqueous phase into the oily phase with constant agitation at 250 RPM, before pouring, both phases also mix by themselves with constant agitation for 10 minutes. According to the qualitative stability tests carried out over time, it was found that all samples met the expected quality criteria, as no air bubbles, sedimentation or creaming were found, all samples showed uniformity in their appearance, with no visible insoluble particles or agglomerations. Centrifugation and thermal stress tests confirmed that the emulsions were stable and there was no phase separation during the 60 days of monitoring. The viscosity, during the 60 days of study, in samples 1, 2 and 4 remained between 4.31 and 4.46 cP; while in the other samples 3 and 5 they showed a slightly higher viscosity, between 4.62 and 4.66 cP. The pH of all batches remained within a slightly acidic range, varying from 6.72 to 6.96 throughout the study period. Regarding the droplet size, only samples 2, 3, 4 and 5 achieved values compatible with the characteristics of a nanoemulsion, with average sizes of 76, 256, 43.5 and 254.3 nm, respectively, fitting a Gaussian distribution. In the thermal stress test, all samples were stable without phase separation during the 60 days of study. In conclusion, samples 2 and 4 show the desired characteristics of stability, droplet size and physicochemical behavior
ABSTRACT This research had the purpose of developing a moisturizing nanoemulsion with Helianthus annuus “sunflower” oil enriched with vitamin E. The samples produced are whitish, odorless and soft to the touch. In the preparation of the nanoemulsions, different proportions of sunflower seed oil, Tween 80 and distilled water were used; but the amount of vitamin E remains constant. 5 samples were worked on, being identified with the numbers 1, 2, 3, 4 and 5. Regarding the procedure, the phase inversion method was used, which consisted of slowly pouring the aqueous phase into the oily phase with constant agitation at 250 RPM, before pouring, both phases also mix by themselves with constant agitation for 10 minutes. According to the qualitative stability tests carried out over time, it was found that all samples met the expected quality criteria, as no air bubbles, sedimentation or creaming were found, all samples showed uniformity in their appearance, with no visible insoluble particles or agglomerations. Centrifugation and thermal stress tests confirmed that the emulsions were stable and there was no phase separation during the 60 days of monitoring. The viscosity, during the 60 days of study, in samples 1, 2 and 4 remained between 4.31 and 4.46 cP; while in the other samples 3 and 5 they showed a slightly higher viscosity, between 4.62 and 4.66 cP. The pH of all batches remained within a slightly acidic range, varying from 6.72 to 6.96 throughout the study period. Regarding the droplet size, only samples 2, 3, 4 and 5 achieved values compatible with the characteristics of a nanoemulsion, with average sizes of 76, 256, 43.5 and 254.3 nm, respectively, fitting a Gaussian distribution. In the thermal stress test, all samples were stable without phase separation during the 60 days of study. In conclusion, samples 2 and 4 show the desired characteristics of stability, droplet size and physicochemical behavior
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Keywords
Nanoemulsión. Helianthus annuus. Tamaño de gota. Cosmético