Efecto de un gel a base de Ipomoea batatas Lam. “camote morado” sobre heridas inducidas en Mus musculus Balb/c
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Date
2021
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Universidad Nacional de Trujillo
Abstract
El presente trabajo tiene por objetivo evaluar el efecto de un gel a base del extracto etanólico de Ipomoea batatas Lam “camote morado” sobre heridas inducidas en Mus musculus Balb/c. Para ello los animales de experimentación se dividieron aleatoriamente en 4 grupos experimentales de 5 especímenes cada uno. A los especímenes se le realizó previamente una herida incisional en el dorso, zona en la que se administraron los tratamientos. Al grupo I (grupo control negativo), no se le aplicó tratamiento; al grupo II, se le aplicó un gel base (formulación que no contiene el compuesto en estudio); al grupo III y IV se aplicó la formulación del gel a base de las cáscaras de las raíces de Ipomoea batatas Lam “camote morado” al 0,5 % y 1 % respectivamente. La aplicación de los tratamientos se realizaron 1 vez al día durante un período de 14 días, durante este periodo se realizaron controles fotográficos y se midieron longitudinalmente las lesiones dérmicas. Posteriormente, se realizó la eutanasia a los animales de experimentación y se tomaron muestras de piel para el estudio histopatológico. Al finalizar el estudio se evidenció la existencia de diferencia estadística significativa en la reducción de la longitud de las lesiones dérmicas entre los grupos experimentales (p<0.05), los grupos que mostraron un mejor efecto fueron los grupos III y IV, estos resultados se correlacionaron con el estudio histopatológico y las observaciones a nivel macroscópico. Se concluyó que los geles a base del extracto etanólico de Ipomoea batatas Lam “camote morado” poseen efecto cicatrizante sobre heridas inducidas en Mus musculus Balb/c, obteniéndose el mayor efecto con el gel al 1 %.
ABSTRACT The present work aims to evaluate the effect of a gel based on the ethanolic extract of Ipomoea batatas Lam "purple sweet potato" on wounds induced in Mus musculus Balb / c. For this, the experimental animals were randomly divided into 4 experimental groups of 5 specimens each, each of these specimens was previously made an incisional wound on the back, the area in which the treatments were administered. To group I (negative control group), no treatment was applied; to group II, a base gel was applied (formulation that does not contain the compound under study); to group III and IV the gel formulation based on the shells of the roots of Ipomoea batatas Lam "purple sweet potato" was applied at 0,5% and 1% respectively. The application of the treatments were carried out once a day during a period of 14 days, during this period photographic controls were carried out and the dermal lesions were measured longitudinally. Subsequently, the experimental animals were euthanized and skin samples were taken for histopathological study. At the end of the study, the existence of a significant statistical difference in the reduction of the length of the dermal lesions was evidenced between the experimental groups (p <0.05), the groups that showed a better effect were groups III and IV, these results were correlated with histopathological study and observations at the macroscopic level. It was concluded that the gels based on the ethanolic extract of Ipomoea batatas Lam "purple sweet potato" have a healing effect on wounds induced in Mus musculus Balb / c, obtaining the greatest effect with the 1% gel.
ABSTRACT The present work aims to evaluate the effect of a gel based on the ethanolic extract of Ipomoea batatas Lam "purple sweet potato" on wounds induced in Mus musculus Balb / c. For this, the experimental animals were randomly divided into 4 experimental groups of 5 specimens each, each of these specimens was previously made an incisional wound on the back, the area in which the treatments were administered. To group I (negative control group), no treatment was applied; to group II, a base gel was applied (formulation that does not contain the compound under study); to group III and IV the gel formulation based on the shells of the roots of Ipomoea batatas Lam "purple sweet potato" was applied at 0,5% and 1% respectively. The application of the treatments were carried out once a day during a period of 14 days, during this period photographic controls were carried out and the dermal lesions were measured longitudinally. Subsequently, the experimental animals were euthanized and skin samples were taken for histopathological study. At the end of the study, the existence of a significant statistical difference in the reduction of the length of the dermal lesions was evidenced between the experimental groups (p <0.05), the groups that showed a better effect were groups III and IV, these results were correlated with histopathological study and observations at the macroscopic level. It was concluded that the gels based on the ethanolic extract of Ipomoea batatas Lam "purple sweet potato" have a healing effect on wounds induced in Mus musculus Balb / c, obtaining the greatest effect with the 1% gel.
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Keywords
Cicatrización de herida. Ipomoea batatas Lam. Camote morado