Efecto de una pomada a base de Solanum tuberosum L. " papa tumbay" sobre quemaduras inducidas en Mus musculus Balb/c
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Date
2021
Authors
Rosas Cruz, Galy Paola
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Publisher
Universidad Nacional de Trujillo
Abstract
El objetivo de este estudio fue evaluar el efecto de una pomada a base de Solanum tuberosum L. “papa tumbay” sobre quemaduras inducidas en Mus musculus Balb/c. Los animales de experimentación se dividieron en 5 grupos con 5 especímenes cada uno, 48 h previo a la inducción de la quemadura de segundo grado, se les realizó la depilación en el área del lomo. Luego se administró tiopental sódico 40mg/kg v.ip., y se procedió a colocar una varilla de metal redonda de 0.7cm de diámetro sobre la piel depilada, a una temperatura de 100°C por 5 segundos. Al Grupo I no se le aplicó ningún tratamiento; al Grupo II se le aplicó una pomada cicatrizante de sulfadiazina de plata 1%, al Grupo III se le aplicó una pomada base y a los otros dos grupos IV y V se le aplicó la pomada formulada a base de Solanum tuberosum L. “papa tumbay” al 1 %, y 2 % respectivamente.
La administración de los tratamientos se realizó una vez al día, durante 21 días. Diariamente se realizó la medición del área de la quemadura y los controles fotográficos respectivos durante todo el periodo experimental. Luego se procedió a realizar la eutanasia utilizando pentobarbital sódico 60 mg/kg v.ip. para obtener muestras de piel que fueron conservadas en formol al 10% y se enviaron a realizar el análisis histopatológico. Los resultados muestran que existe diferencia estadísticamente significativa entre los grupos, con un nivel de significancia de p<0,05 durante el tiempo de experimentación. En los cortes histopatológicos se observó que en los grupos IV y V hay presencia de abundante colágeno y fibroblastos en dirección a la dermis, atribuible al efecto de las pomadas. Se concluye que las pomadas a base de extracto etanólico de Solanum tuberosum L. “papa tumbay” poseen efecto cicatrizante sobre quemaduras de segundo grado inducidas en Mus musculus Balb/c, siendo el mayor efecto con la pomada al 2%
The objective of this study was to evaluate the effect of an ointment based on Solanum tuberosum L. "papa tumbay" on burns induced in Mus musculus Balb / c. The experimental animals were divided into 5 groups with 5 specimens each, 48 h prior to the induction of second degree burn, depilation was performed in the area of the back. Then sodium thiopental 40mg / kg v.ip. was administered, and a 0.7cm diameter round metal rod was placed on the depilated skin, at a temperature of 100 ° C for 5 seconds. Group I did not receive any treatment; Group II received a 1% silver sulfadiazine healing ointment, Group III received a base ointment and the other two groups IV and V received the ointment formulated based on Solanum tuberosum L. "papa tumbay" at 1%, and 2% respectively. The administration of the treatments was carried out once a day, for 21 days. Measurement of the burn area and the respective photographic controls were carried out daily throughout the experimental period. Then, euthanasia was carried out using sodium pentobarbital 60 mg / kg v.ip. to obtain skin samples that were preserved in 10% formalin and sent for histopathological analysis. The results show that there is a statistically significant difference between the groups, with a significance level of p <0.05 during the experimentation time. In the histopathological sections, it was observed that in groups IV and V there is abundant collagen and fibroblasts in the direction of the dermis, attributable to the effect of the ointments. It is concluded that ointments based on ethanolic extract of Solanum tuberosum L. "papa tumbay" have a healing effect on second degree burns induced in Mus musculus Balb / c, the greatest effect being with the 2% ointment.
The objective of this study was to evaluate the effect of an ointment based on Solanum tuberosum L. "papa tumbay" on burns induced in Mus musculus Balb / c. The experimental animals were divided into 5 groups with 5 specimens each, 48 h prior to the induction of second degree burn, depilation was performed in the area of the back. Then sodium thiopental 40mg / kg v.ip. was administered, and a 0.7cm diameter round metal rod was placed on the depilated skin, at a temperature of 100 ° C for 5 seconds. Group I did not receive any treatment; Group II received a 1% silver sulfadiazine healing ointment, Group III received a base ointment and the other two groups IV and V received the ointment formulated based on Solanum tuberosum L. "papa tumbay" at 1%, and 2% respectively. The administration of the treatments was carried out once a day, for 21 days. Measurement of the burn area and the respective photographic controls were carried out daily throughout the experimental period. Then, euthanasia was carried out using sodium pentobarbital 60 mg / kg v.ip. to obtain skin samples that were preserved in 10% formalin and sent for histopathological analysis. The results show that there is a statistically significant difference between the groups, with a significance level of p <0.05 during the experimentation time. In the histopathological sections, it was observed that in groups IV and V there is abundant collagen and fibroblasts in the direction of the dermis, attributable to the effect of the ointments. It is concluded that ointments based on ethanolic extract of Solanum tuberosum L. "papa tumbay" have a healing effect on second degree burns induced in Mus musculus Balb / c, the greatest effect being with the 2% ointment.
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Keywords
Cicatrización de quemaduras. Pomada. Solanum tuberosum L, papa