Efecto del extracto de Ipomoea batatas sobre el desarrollo tumoral en Rattus norvegicus var. albinus con cáncer de mama inducido
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Date
2022
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Universidad Nacional de Trujillo
Abstract
El presente trabajo tuvo como finalidad evaluar el efecto del extracto etanólico de
Ipomoea batatas sobre cáncer de mama inducido en ratas. Para ello se preparó el extracto
etanólico utilizando las cáscaras de las raíces de I. batatas, dejando macerar en etanol 96°
acidificado a pH 3,5. Se conformó 4 grupos experimentales: Grupo Control DMBA:
conformado por 10 Rattus norvegicus var. albinus, hembras, que recibieron 7,12-
dimetilbenzo (α) antraceno (DMBA) en una sola dosis de 20 mg vía subcutánea y solución
salina fisiológica vía oral por 4 meses, Grupos problemas I, II y III: Conformado cada
uno por 10 Rattus norvegicus var. albinus, hembras, que recibieron una sola dosis de
DMBA y 200, 400 y 600 mg/kg/día vía oral del extracto de I. batatas, respectivamente,
por 4 meses. Los especímenes fueron evaluados, semanalmente mediante palpación a
nivel de la pared toracoabdominal y la región inguinal, para detectar la presencia de
tumores y registrar el tiempo de aparición de los tumores mamarios (latencia), luego de
ello, los animales fueron eutanizados por sobredosis de pentobarbital, procediendo a
extraer los tumores para determinar el volumen de cada tumor y realizar el análisis
histopatológico. Los resultados evidenciaron que el grupo control DMBA presentó
desarrollo tumoral mamario, con características histológicas de carcinoma ductal in situ
y presencia de áreas necróticas, mientras que los grupos que recibieron el extracto de I.
batatas presentaron una disminución del volumen tumoral y a nivel histológico mostraron
recuperación del conducto ductal y no se evidenciaron áreas de necrosis. Se concluye que
el extracto etanólico de las raíces de Ipomoea batatas produce disminución del desarrollo
tumoral en Rattus norvegicus var. albinus con cáncer de mama inducido, siendo este
efecto dosis dependiente.
The purpose of this study was to evaluate the effect of the ethanolic extract of Ipomoea batatas on induced breast cancer in rats. For this, the ethanolic extract was prepared using the husks of the roots of I. batatas, leaving them to macerate in 96° ethanol acidified to pH 3.5. Four experimental groups were divided: DMBA Control Group: made up of 10 Rattus norvegicus var. albinus, females, who received 7,12-dimethylbenzo (α) anthracene (DMBA) in a single dose of 20 mg subcutaneously and physiological saline solution orally for 4 months, Problem groups I, II and III: Each one made up of 10 Rattus norvegicus var. albinus, females, who received a single dose of DMBA and 200, 400 and 600 mg/kg/day orally of the I. batatas extract, respectively, for 4 months. The specimens were evaluated weekly by palpation at the level of the thoracoabdominal wall and the inguinal region, to detect the presence of tumors and record the time of appearance of mammary tumors (latency), after which, the animals were euthanized by an overdose of pentobarbital, proceeding to extract the tumors to determine the volume of each tumor and perform the histopathological analysis. The results showed that the DMBA control group presented mammary tumor development with histological characteristics of ductal carcinoma in situ and the presence of necrotic areas. Still, the groups that received the I. batatas extract demonstrated a decrease in tumor volume and, at the histological level, showed recovery of the duct. ductal and no areas of necrosis were evident. It is concluded that the ethanolic extract of the roots of Ipomoea batatas produces a decrease in tumor development in Rattus norvegicus var. albinus with induced breast cancer, this effect being dose-dependent.
The purpose of this study was to evaluate the effect of the ethanolic extract of Ipomoea batatas on induced breast cancer in rats. For this, the ethanolic extract was prepared using the husks of the roots of I. batatas, leaving them to macerate in 96° ethanol acidified to pH 3.5. Four experimental groups were divided: DMBA Control Group: made up of 10 Rattus norvegicus var. albinus, females, who received 7,12-dimethylbenzo (α) anthracene (DMBA) in a single dose of 20 mg subcutaneously and physiological saline solution orally for 4 months, Problem groups I, II and III: Each one made up of 10 Rattus norvegicus var. albinus, females, who received a single dose of DMBA and 200, 400 and 600 mg/kg/day orally of the I. batatas extract, respectively, for 4 months. The specimens were evaluated weekly by palpation at the level of the thoracoabdominal wall and the inguinal region, to detect the presence of tumors and record the time of appearance of mammary tumors (latency), after which, the animals were euthanized by an overdose of pentobarbital, proceeding to extract the tumors to determine the volume of each tumor and perform the histopathological analysis. The results showed that the DMBA control group presented mammary tumor development with histological characteristics of ductal carcinoma in situ and the presence of necrotic areas. Still, the groups that received the I. batatas extract demonstrated a decrease in tumor volume and, at the histological level, showed recovery of the duct. ductal and no areas of necrosis were evident. It is concluded that the ethanolic extract of the roots of Ipomoea batatas produces a decrease in tumor development in Rattus norvegicus var. albinus with induced breast cancer, this effect being dose-dependent.
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Keywords
Cáncer, DMBA, Antocianinas, Rata, Tumoral