Ascorbato y derivados de vitamina K3: Evaluación in vitro del efecto citotóxico en células cancerígenas humanas
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Date
2021
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Universidad Nacional de Trujillo
Abstract
En base a la actividad anticancerígena de la vitamina K3 (menadiona), cuando se usa como tratamiento único y combinado con ascorbato de sodio, se determinó la actividad citotóxica de los derivados de vitamina K3 (MDN1, MDN2, ENQN1, ENQN2), los cuales fueron sometidos in vitro contra células cancerosas humanas (próstata DU-145, vejiga T-24 y mama MCF-7, MDA-MB-231), y células de riñón embrionario humano no tumoral HEK-293 usando el método colorimétrico MTT. Los valores de IC50 (mM) de MDN1 y MDN2 fueron los de mejor resultado, destacando en la línea celular DU-145, indicando un efecto citotóxico selectivo de estas moléculas hacia este tipo de células cancerosas. Se evaluó en ellas su viabilidad celular, luego de ser expuestas a diversas concentraciones de MDN1, MDN2, y en combinación con ascorbato, a diferentes tiempos de tratamiento. La viabilidad disminuyó significativamente cuando fue adicionado el ascorbato, probándose el efecto citotóxico sinérgico de esta combinación. MDN2/ascorbato fue la más potente para reducir la viabilidad de las células DU-145 por debajo del 50% reducción MTT. En conclusión, la combinación MDN2/ascorbato, muestra una potencial aplicación para seguir investigando y evaluar terapias futuras específicamente contra el cáncer de próstata
ABSTRACT Based on anticancer activity of vitamin K3 (menadione), when used as a single treatment and combined with sodium ascorbate, the cytotoxic activity of vitamin K3 derivatives (MDN1, MDN2, ENQN1, ENQN2) which were subjected in vitro against human cancer cells (DU-145 prostate, T-24 bladder and MCF-7, MDA-MB-231 breast), and HEK-293 non-tumor human embryonic kidney cells using the MTT colorimetric method. The IC50 (mM) values of MDN1 and MDN2 were the best results, standing out in the DU-145 cell line, indicating a selective cytotoxic effect of these molecules towards this type of cancer cells. Cell viability was evaluated after being exposed to different concentrations of MDN1, MDN2, and in combination with ascorbate, at different treatment times. Viability decreased significantly when ascorbate was added, proving the synergistic cytotoxic effect of this combination. MDN2/ascorbate was the most potent in reducing the viability of DU-145 cells below 50 % MTT reduction. In conclusion, the MDN2/ascorbate combination shows a potential application for further research and evaluation of future therapies specifically against prostate cancer
ABSTRACT Based on anticancer activity of vitamin K3 (menadione), when used as a single treatment and combined with sodium ascorbate, the cytotoxic activity of vitamin K3 derivatives (MDN1, MDN2, ENQN1, ENQN2) which were subjected in vitro against human cancer cells (DU-145 prostate, T-24 bladder and MCF-7, MDA-MB-231 breast), and HEK-293 non-tumor human embryonic kidney cells using the MTT colorimetric method. The IC50 (mM) values of MDN1 and MDN2 were the best results, standing out in the DU-145 cell line, indicating a selective cytotoxic effect of these molecules towards this type of cancer cells. Cell viability was evaluated after being exposed to different concentrations of MDN1, MDN2, and in combination with ascorbate, at different treatment times. Viability decreased significantly when ascorbate was added, proving the synergistic cytotoxic effect of this combination. MDN2/ascorbate was the most potent in reducing the viability of DU-145 cells below 50 % MTT reduction. In conclusion, the MDN2/ascorbate combination shows a potential application for further research and evaluation of future therapies specifically against prostate cancer
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Keywords
Vitamina K3. Ascorbato de sodio. Actividad citotóxica. Viabilidad celular. Efecto citotóxico sinérgico. Cáncer de próstata