Efecto de las concentraciones de extractos acuosos de semillas de Piper nigrum y Cuminum cyminum sobre la toxicidad en Artemia salina in vitro
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Objetivo: Evaluar el efecto toxico de los extractos acuosos de semillas de Piper nigrum L. y Cuminum cyminum L. frente a Artemia salina in vitro. Metodología: Se basó en un estudio de tipo cuantitativo, prospectivo de diseño experimental con grupos control, la población biológica estuvo conformada por las semillas de Piper. nigrum L. y Cuminum cyminun L. y los huevos de Artemia salina L. a quienes se les aplico el ensayo de letalidad en larvas de Artemia salina, para determinar el efecto toxico de las plantas a las concentraciones de 1:10 (100mg/mL); 1:20 (50mg/mL); 1:40 (25mg/mL); 1:80 (12.5mg/mL); 1:160 (6.25mg/mL); 1:320 (3.13mg/mL); 1:640 (1.56mg/mL); 1:960 (1.04mg/mL). Resultados: Se encontró una mortalidad del 100% a las concentraciones de 100 mg/mL y 50 mg/mL, tanto para el extracto acuoso de semillas de Piper nigrum L. y Cuminum cyminum L. La CL50 del extracto acuoso de las semillas de Piper nigrum L. mediante la regresión logarítmica y probit fue de 5.84 mg/mL y de 5.38 mg/mL respectivamente y la CL50 de extracto acuoso de semillas de Cuminum cyminum L. mediante la regresión logarítmica y probit fue de 4.50 mg/mL y 6.035 mg/mL respectivamente. Conclusión: Se determinó que tanto Piper nigrum L. como Cuminum cyminum L., son toxicas para Artemia salina a altas concentraciones.
Objective: To evaluate the toxic effect of the aqueous extracts of seeds of Piper nigrum L. and Cuminum cyminum L. against Artemia salina in vitro. Methodology: It was based on a quantitative study, prospective experimental design with control groups, the biological population was made up of the seeds of P. nigrum L. and C. cyminun L. and the eggs of Artemia salina L. I applied the lethality test on Arternia salina larvae, to determine the toxic effect of the plants at concentrations of 1:10 (100mg/mL); 1:20 (50mg/mL); 1:40 (25mg/mL); 1:80 (12.5mg/mL); 1:160 (6.25mg/mL); 1:320 (3.13mg/mL); 1:640 (1.56mg/mL); 1:960 (1.04mg/mL). Results: A mortality of 100% was found at concentrations of 100 mg/mL and 50 mg/mL, both for the aqueous extract of Piper nigrum L. and Cuminum cyminum L. The LC50 of the aqueous extract of Piper seeds nigrum L. by logarithmic and probit regression was 5.84 mg/mL and 5.38 mg/mL respectively and the LC50 of aqueous extract of Cuminum cyminum L. seeds by logarithmic and probit regression was 4.50 mg/mL and 6.035 mg /mL respectively. Conclusion: It was determined that both Piper nigrum L. and Cominum cyminum L. are toxic to Artemia salina at high concentrations.
Objective: To evaluate the toxic effect of the aqueous extracts of seeds of Piper nigrum L. and Cuminum cyminum L. against Artemia salina in vitro. Methodology: It was based on a quantitative study, prospective experimental design with control groups, the biological population was made up of the seeds of P. nigrum L. and C. cyminun L. and the eggs of Artemia salina L. I applied the lethality test on Arternia salina larvae, to determine the toxic effect of the plants at concentrations of 1:10 (100mg/mL); 1:20 (50mg/mL); 1:40 (25mg/mL); 1:80 (12.5mg/mL); 1:160 (6.25mg/mL); 1:320 (3.13mg/mL); 1:640 (1.56mg/mL); 1:960 (1.04mg/mL). Results: A mortality of 100% was found at concentrations of 100 mg/mL and 50 mg/mL, both for the aqueous extract of Piper nigrum L. and Cuminum cyminum L. The LC50 of the aqueous extract of Piper seeds nigrum L. by logarithmic and probit regression was 5.84 mg/mL and 5.38 mg/mL respectively and the LC50 of aqueous extract of Cuminum cyminum L. seeds by logarithmic and probit regression was 4.50 mg/mL and 6.035 mg /mL respectively. Conclusion: It was determined that both Piper nigrum L. and Cominum cyminum L. are toxic to Artemia salina at high concentrations.
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Keywords
Piper nigrum L., Cuminum cyminum L., Artemia salina, Concentración letal 50, CL50