USO DE UN PRE SELLADOR A BASE DE ACIDO LÁCTICO Y DE UN POST SELLADOR A BASE DE CLORHEXIDINA DIGLUCONATO PARA EL CONTROL Y PREVENCIÓN DE MASTITIS SUBCLÍNICA DE VACAS LECHERAS EN LA UNIDAD AGROPECUARIA LOS LUISES S.R.L. - TRUJILLO
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Date
2019
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Universidad Nacional de Trujillo
Abstract
La presente investigación se realizó con el objetivo evaluar el control y prevención de mastitis subclínica en vacas lecheras utilizando un pre sellador a base de ácido láctico al 40% y de un post sellador a base de clorhexidina digluconato al 0,5% en la unidad agropecuaria Los Luises S.R.L., ubicada en el distrito de Huanchaco. Para ello, se recolectaron 340 muestras de leche de cuartos individuales provenientes de 90 vacas presuntamente sanas. A todas las muestras se les realizó la prueba CMT (California Mastitis Test) con el fin de determinar la Prevalencia de Mastitis. Los tratamientos utilizados fueron; tratamiento de control con pre sellador y pos sellador a base de yodo convencional T(0), tratamiento con pre sellador a base de ácido láctico (<10%) y pos sellador a base de yodo (3mg/g.) T(1), y el tratamiento con pre sellador a base de ácido láctico (40%) y pos sellador a base de Clorhexidina digluconato (0,5%) T(2). Se utilizó el diseño completamente aleatorio (DCA), con 3 tratamientos y 6 repeticiones. De acuerdo con los resultados obtenidos, al final del T (0) se encontró una prevalencia de mastitis subclínica del 70,0% correspondiente a 21 vacas lecheras para un total de 30 vacas en producción tratadas con pre sellador y pos sellador a base de yodo convencional. Así mismo, al aplicar el T (1) con pre sellador a base de ácido láctico (<10%) y pos sellador a base de yodo (3mg/g.), se observa que una prevalencia de mastitis subclínica del 79,3% correspondiente a 23 vacas afectadas de un total de 29 evaluadas. Sin embargo, al aplicar el T (2) con pre sellador a base de ácido láctico (40%) y pos sellador a base de Clorhexidina digluconato (0,5%), muestra que hubo una disminución de 69,2% de la población estimada que padecía de mastitis subclínica con respecto al inicio del tratamiento, pues la prevalencia de mastitis subclínica fue 23,1% correspondiente a 6 vacas afectadas de 26 evaluadas. Mediante la prueba exacta de Fisher se determinó que la prevalencia o ausencia de mastitis depende del tratamiento usado, la misma que muestra un valor de p (<0,05). Por lo tanto, los resultados permiten concluir que el uso de un pre sellador a base de ácido láctico al 40% y de un post sellador a base de clorhexidina digluconato al 0,5% tiene un efecto positivo para para el control y prevención de mastitis subclínica de las vacas lecheras.
ABSTRACT The present investigation was carried out with the objective of evaluating the control and prevention of subclinical mastitis in dairy cows using a pre-sealer based on 40% lactic acid and a 0,5% chlorhexidine digluconate base post in the Los Luises agricultural unit SRL, located in the district of Huanchaco. For this, 340 milk samples were collected from individual rooms from 90 presumably healthy cows. All the samples were subjected to the CMT (California Mastitis Test) in order to determine the Prevalence of Subclinical Mastitis and the correlation of the period of lactation with this and the affected mammary rooms. The treatments used were; control treatment with pre-sealer and post-sealer based on conventional iodine T (0), treatment with pre-sealer based on lactic acid (<10%) and post-sealer based on iodine (3mg / g.) T (1) , and the treatment with pre-sealer based on lactic acid (40%) and postage based on Chlorhexidine digluconate (0,5%) T (2). The completely randomized design (DCA) was used, with 3 treatments and 6 repetitions. According to the results obtained, at the end of T (0) a prevalence of subclinical mastitis of 70,0% corresponding to 21 dairy cows was found for a total of 30 cows in production treated with pre-sealer and post sealer based on conventional iodine. Likewise, when applying the T (1) with pre-sealer based on lactic acid (<10%) and post-sealer based on iodine (3mg / g.), It is observed that a prevalence of subclinical mastitis of 79,3% corresponding to 23 affected cows out of a total of 29 evaluated. However, when applying T (2) with pre-sealer based on lactic acid (40%) and postage based on Chlorhexidine digluconate (0,5%), it shows that there was a decrease of 69,2% of the estimated population suffering from subclinical mastitis with respect to the start of treatment, since the prevalence of subclinical mastitis was 23,1% corresponding to 6 affected cows of 26 evaluated. Using Fisher's exact test, it was determined that the prevalence or absence of mastitis depends on the treatment used, which shows a value of p (<0,05). Therefore, the results allow us to conclude that the use of a pre-sealer based on 40% lactic acid and a post-sealer based on chlorhexidine digluconate at 0,5% has a positive effect for the control and prevention of subclinical mastitis of the dairy cows.
ABSTRACT The present investigation was carried out with the objective of evaluating the control and prevention of subclinical mastitis in dairy cows using a pre-sealer based on 40% lactic acid and a 0,5% chlorhexidine digluconate base post in the Los Luises agricultural unit SRL, located in the district of Huanchaco. For this, 340 milk samples were collected from individual rooms from 90 presumably healthy cows. All the samples were subjected to the CMT (California Mastitis Test) in order to determine the Prevalence of Subclinical Mastitis and the correlation of the period of lactation with this and the affected mammary rooms. The treatments used were; control treatment with pre-sealer and post-sealer based on conventional iodine T (0), treatment with pre-sealer based on lactic acid (<10%) and post-sealer based on iodine (3mg / g.) T (1) , and the treatment with pre-sealer based on lactic acid (40%) and postage based on Chlorhexidine digluconate (0,5%) T (2). The completely randomized design (DCA) was used, with 3 treatments and 6 repetitions. According to the results obtained, at the end of T (0) a prevalence of subclinical mastitis of 70,0% corresponding to 21 dairy cows was found for a total of 30 cows in production treated with pre-sealer and post sealer based on conventional iodine. Likewise, when applying the T (1) with pre-sealer based on lactic acid (<10%) and post-sealer based on iodine (3mg / g.), It is observed that a prevalence of subclinical mastitis of 79,3% corresponding to 23 affected cows out of a total of 29 evaluated. However, when applying T (2) with pre-sealer based on lactic acid (40%) and postage based on Chlorhexidine digluconate (0,5%), it shows that there was a decrease of 69,2% of the estimated population suffering from subclinical mastitis with respect to the start of treatment, since the prevalence of subclinical mastitis was 23,1% corresponding to 6 affected cows of 26 evaluated. Using Fisher's exact test, it was determined that the prevalence or absence of mastitis depends on the treatment used, which shows a value of p (<0,05). Therefore, the results allow us to conclude that the use of a pre-sealer based on 40% lactic acid and a post-sealer based on chlorhexidine digluconate at 0,5% has a positive effect for the control and prevention of subclinical mastitis of the dairy cows.
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Keywords
Mastitis subclínica, Acido láctico, clorhexidina digluconato, Pre sellador, Vacas lecheras