EFECTO DEL EXTRACTO HIDROALCOHÓLICO DE Tropaeolum tuberosum SOBRE ÚLCERAS GÁSTRICAS INDUCIDAS EN Mus musculus
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue determinar el efecto gastroprotector del extracto hidroalcohólico Tropaeolum tuberosum sobre úlceras gástricas inducidas en Mus musculus. Del tubérculo de Tropaeolum tuberosum “mashua amarilla” y se obtuvo el extracto hidroalcohólico con etanol de 96 %; se indujo úlcera gástrica con 50 mg/Kg de peso corporal (pc) de indometacina a cada uno de los individuos, excepto a los del grupo control negativo; se formaron siete grupos, cada uno conformado por seis ratones hembra de 16 a 18 semanas de edad: grupo control negativo (GCNEG), grupo control positivo (GCPOS) sin tratamiento, grupo estándar ranitidina (GERAN) tratado con 50 mg/Kg pc de ranitidina, grupo estándar omeprazol (GEOME) tratado con 50 mg/Kg pc de omeprazol, mashua amarilla 100 (MA100) tratado con 100 mg/Kg pc de extracto, mashua amarilla 200 (MA200) tratado con 200 mg/Kg pc de extracto, mashua amarilla 400 (MA400) tratado con 400 mg/Kg pc de extracto; se determinó el porcentaje de inhibición de la úlcera gástrica usando la escala de Marhuenda y se midió el pH gástrico, los datos obtenidos fueron sometidos a un análisis de varianza
(ANOVA) y a una prueba Tukey, ambos con 95% de confianza. Los resultados: GCNEG=64,86 ± 15,94% de inhibición y pH gástrico=3,17 ± 0,41; GCPOS=0,00 ± 0,00% de inhibición y pH gástrico=2,75 ± 0,69; GERAN=64,86 ± 15,94% de inhibición y pH gástrico=3,33 ± 0,88; GEOME=78,38 ± 8,37% de inhibición y pH gástrico=3,42 ± 0,97; MA100=7,57 ± 10,26 % de inhibición y pH gástrico=2,92 ± 0,58; MA200=72,97 ± 13,24 % inhibición y pH=3,83 ± 0,68 y MA400=70,27 ± 6,62 % inhibición y pH gástrico=1,75 ± 0,94. Se concluye que el extracto hidroalcohólico de Tropaeolum tuberosum a dosis de 100, 200 y 400 mg/Kg pc posee efecto gastroprotector sobre úlceras gástricas inducidas en mus musculus;
siendo la dosis de 200 mg/Kg pc la que mejor efecto muestra.
The objective of this research was to determine the gastroprotective effect of the hydroalcoholic extract Tropaeolum tuberosum on gastric ulcers induced in Mus musculus. From the tuber of Tropaeolum tuberosum “yellow mashua” and the hydroalcoholic extract was obtained with 96% ethanol; gastric ulcer was induced with 50 mg/kg body weight (pc) of indomethacin in each of the individuals, except those in the negative control group; Seven groups were formed, each consisting of six female mice of 16 to 18 weeks of age: negative control group (GCNEG), positive control group (GCPOS) without treatment, standard ranitidine group (GERAN) treated with 50 mg/Kg pc of ranitidine, standard group omeprazole (GEOME) treated with 50 mg/Kg pc of omeprazole, yellow mashua 100 (MA100) treated with 100 mg/Kg pc of extract, yellow mashua 200 (MA200) treated with 200 mg/Kg pc of extract, yellow mashua 400 (MA400) treated with 400 mg/Kg pc of extract; The percentage of inhibition of gastric ulcer was determined using the Marhuenda scale and gastric pH was measured. The data obtained were subjected to an analysis of variance (ANOVA) and a Tukey test, both with 95% confidence. The results: GCNEG=64.86 ± 15.94% inhibition and gastric pH=3.17 ± 0.41; GCPOS=0.00 ± 0.00% inhibition and gastric pH=2.75 ± 0.69; GERAN=64.86 ± 15.94% inhibition and gastric pH=3.33 ± 0.88; GEOME=78.38 ± 8.37% inhibition and gastric pH=3.42 ± 0.97; MA100=7.57 ± 10.26 % inhibition and gastric pH=2.92 ± 0.58; MA200=72.97 ± 13.24 % inhibition and pH=3.83 ± 0.68 and MA400=70.27 ± 6.62 % inhibition and gastric pH=1.75 ± 0.94. It is concluded that the hydroalcoholic extract of Tropaeolum tuberosum at doses of 100, 200 and 400 mg/Kg pc has a gastroprotective effect on gastric ulcers induced in Mus musculus; The dose of 200 mg/Kg pc being the one that shows the best effect.
The objective of this research was to determine the gastroprotective effect of the hydroalcoholic extract Tropaeolum tuberosum on gastric ulcers induced in Mus musculus. From the tuber of Tropaeolum tuberosum “yellow mashua” and the hydroalcoholic extract was obtained with 96% ethanol; gastric ulcer was induced with 50 mg/kg body weight (pc) of indomethacin in each of the individuals, except those in the negative control group; Seven groups were formed, each consisting of six female mice of 16 to 18 weeks of age: negative control group (GCNEG), positive control group (GCPOS) without treatment, standard ranitidine group (GERAN) treated with 50 mg/Kg pc of ranitidine, standard group omeprazole (GEOME) treated with 50 mg/Kg pc of omeprazole, yellow mashua 100 (MA100) treated with 100 mg/Kg pc of extract, yellow mashua 200 (MA200) treated with 200 mg/Kg pc of extract, yellow mashua 400 (MA400) treated with 400 mg/Kg pc of extract; The percentage of inhibition of gastric ulcer was determined using the Marhuenda scale and gastric pH was measured. The data obtained were subjected to an analysis of variance (ANOVA) and a Tukey test, both with 95% confidence. The results: GCNEG=64.86 ± 15.94% inhibition and gastric pH=3.17 ± 0.41; GCPOS=0.00 ± 0.00% inhibition and gastric pH=2.75 ± 0.69; GERAN=64.86 ± 15.94% inhibition and gastric pH=3.33 ± 0.88; GEOME=78.38 ± 8.37% inhibition and gastric pH=3.42 ± 0.97; MA100=7.57 ± 10.26 % inhibition and gastric pH=2.92 ± 0.58; MA200=72.97 ± 13.24 % inhibition and pH=3.83 ± 0.68 and MA400=70.27 ± 6.62 % inhibition and gastric pH=1.75 ± 0.94. It is concluded that the hydroalcoholic extract of Tropaeolum tuberosum at doses of 100, 200 and 400 mg/Kg pc has a gastroprotective effect on gastric ulcers induced in Mus musculus; The dose of 200 mg/Kg pc being the one that shows the best effect.
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Keywords
Tropaeolum tuberosum, úlcera gástrica, protección gástrica