Fitoquímica y capacidad antioxidante del aceite esencial de las raíces de Valeriana pilosa Ruiz & Pav.
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente trabajo de investigación identificó la composición fitoquímica y evalúa la capacidad antioxidante del aceite esencial (AE) de las raíces de Valeriana pilosa Ruiz & Pav, procedente de los distritos de Calquiz (Cajamarca) y Usquil (La Libertad). Se identificaron mediante GC-MS, 15 compuestos fitoquímicos, del AE procedentes del distrito de Calquiz, los más notables son alcohol de pachuli, α– pachuleno y seychellene, en el AE procedente del distrito Usquil se identificó 23 compuestos fitoquímicos los más importantes el α– pachuleno, α– gurjuneno, seychellene y alcohol de pachuli. La capacidad antioxidante se determinó por el método de Brand-Williams de reducción de radicales libres DPPH•, las concentraciones de AE usadas fueron de 1,61; 3,125 y 4,54 %, donde el AE del distrito de Calquiz mostró un porcentaje de reducción de 16,80; 31,22 y 42,68 % respectivamente con un IC50 de 5184,30 µg/mL, respectivamente. Por su parte, el AE del distrito de Usquil presentó porcentajes de reducción de 28,79; 42,33 y 53,54 % con un IC50 de 3928,62 µg/mL.
La capacidad antioxidante FRAP se determinó a las concentraciones de 25, 50 y 75 %, mostrando que el AE del distrito de Calquiz tiene un porcentaje de reducción de 0,049; 2,77 y 4,88% y el IC50 de 4854,01 µg/mL equivalente a Trolox. Mientras que el AE del distrito de Usquil mostró porcentajes de reducción de 0,013; 4, 88 y 10,45 %; con un IC50 de 2079,94 µg/mL, equivalente a Trolox. En conclusión, los resultados del AE de las raíces de V. pilosa Ruiz & Pav, del distrito de Usquil presentó mayor capacidad antioxidante, en comparación con el AE procedente del distrito de Calquiz.
ABSTRACT This research work identified the phytochemical composition and evaluated the antioxidant capacity of the essential oil (EO) of the roots of Valeriana pilosa Ruiz & Pav, from the districts of Calquiz (Cajamarca) and Usquil (La Libertad). By GC-MS, 15 phytochemical compounds were identified in the EO from the Calquiz district, the most notable being patchouli alcohol, α- patchoulene and seychellene. In the EO from the Usquil district, 23 phytochemical compounds were identified, the most important being α- patchoulene, α- gurjunene, seychellene and patchouli alcohol. The antioxidant capacity was determined by the Brand-Williams free radical reduction method DPPH•, the EO concentrations used were 1.61; 3,125 and 4.54 %, where the EO from the Calquiz district showed a reduction percentage of 16.80; 31.22 and 42.68 % respectively with an IC50 of 5184.30 µg/mL, respectively. For its part, the EO from the Usquil district presented reduction percentages of 28.79; 42.33 and 53.54 % with an IC50 of 3928.62 µg/mL. The FRAP antioxidant capacity was determined at concentrations of 25, 50 and 75 %, showing that the EO from the Calquiz district has a reduction percentage of 0.049; 2.77 and 4.88% and the IC50 of 4854.01 µg/mL equivalent to Trolox. While the EO from the Usquil district showed reduction percentages of 0.013; 4, 88 and 10.45 %; with an IC50 of 2079.94 µg/mL, equivalent to Trolox. In conclusion, the results of the EO from the roots of V. pilosa Ruiz & Pav, from the Usquil district presented a higher antioxidant capacity, compared to the EO from the Calquiz district.
ABSTRACT This research work identified the phytochemical composition and evaluated the antioxidant capacity of the essential oil (EO) of the roots of Valeriana pilosa Ruiz & Pav, from the districts of Calquiz (Cajamarca) and Usquil (La Libertad). By GC-MS, 15 phytochemical compounds were identified in the EO from the Calquiz district, the most notable being patchouli alcohol, α- patchoulene and seychellene. In the EO from the Usquil district, 23 phytochemical compounds were identified, the most important being α- patchoulene, α- gurjunene, seychellene and patchouli alcohol. The antioxidant capacity was determined by the Brand-Williams free radical reduction method DPPH•, the EO concentrations used were 1.61; 3,125 and 4.54 %, where the EO from the Calquiz district showed a reduction percentage of 16.80; 31.22 and 42.68 % respectively with an IC50 of 5184.30 µg/mL, respectively. For its part, the EO from the Usquil district presented reduction percentages of 28.79; 42.33 and 53.54 % with an IC50 of 3928.62 µg/mL. The FRAP antioxidant capacity was determined at concentrations of 25, 50 and 75 %, showing that the EO from the Calquiz district has a reduction percentage of 0.049; 2.77 and 4.88% and the IC50 of 4854.01 µg/mL equivalent to Trolox. While the EO from the Usquil district showed reduction percentages of 0.013; 4, 88 and 10.45 %; with an IC50 of 2079.94 µg/mL, equivalent to Trolox. In conclusion, the results of the EO from the roots of V. pilosa Ruiz & Pav, from the Usquil district presented a higher antioxidant capacity, compared to the EO from the Calquiz district.
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Keywords
Valeriana pilosa Ruiz & Pav. Fitoquímica. DPPH•. FRAP.