Efecto de la concentración y tiempo de exposición del dióxido de cloro en el crecimiento de Salmonella enterica, Pseudomonas aeruginosa y Staphylococcus aureus
No Thumbnail Available
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
El presente estudio tuvo como objetivo evaluar el efecto del dióxido de cloro (ClO2) en el crecimiento de Salmonella enterica, Pseudomonas aeruginosa y Staphylococcus aureus en condiciones de laboratorio. Las pruebas se realizaron utilizando concentraciones de ClO2 de 5ppm, 10ppm y 15ppm, con diferentes tiempos de exposición de 5, 10 y 15 min, y se llevó a cabo por triplicado para cada variable de tiempo, concentración y cepa bacteriana. Se determino la reducción bacteriana, y para ello, se realizaron recuentos de unidades formadoras de colonias (UFC) y se calculó el porcentaje de reducción mediante un promedio aritmético de los resultados obtenidos. Además, se realizó un análisis estadístico ANOVA para determinar las diferencias significativas en la reducción bacteriana entre los diferentes tratamientos. Los resultados obtenidos revelaron que el dióxido de cloro muestra un efecto significativo en la reducción del crecimiento de las tres cepas bacterianas evaluadas; siendo las concentraciones de 10ppm,15ppm a 15min de exposición donde se obtuvieron mayor porcentaje de reducción bacteriana; para Salmonella enterica se redujo en un 87% y 96.50%, Pseudomonas aeruginosa 77.40% y 95.35% y Staphylococcus aureus 87.60% y 98.73% respectivamente. Se encontró que el efecto del ClO2 varió según la concentración y el tiempo de exposición, esto es, a mayor tiempo de exposición una mayor reducción en el crecimiento bacteriano en comparación con los tiempos de exposición a 5min a concentraciones de 5ppm donde se obtuvo menor reducción bacteriana; para Salmonella entérica 17,7%, Pseudomonas aeruginosa 12.03% y Staphylococcus aureus 18,23%.
The present study aimed to evaluate the effect of chlorine dioxide (ClO2) on the growth of Salmonella enterica, Pseudomonas aeruginosa and Staphylococcus aureus under laboratory conditions. The tests were performed using ClO2 concentrations of 5ppm, 10ppm and 15ppm, with different exposure times of 5, 10 and 15 min, and were carried out in triplicate for each variable of time, concentration and bacterial strain. Bacterial reduction was determined by counting colony forming units (CFU) and calculating the percentage of reduction by arithmetic average of the results obtained. In addition, an ANOVA statistical analysis was performed to determine the significant differences in bacterial reduction between the different treatments. The results obtained revealed that chlorine dioxide showed a significant effect in the reduction of the growth of the three bacterial strains evaluated; being the concentrations of 10ppm, 15ppm and 15min of exposure where the highest percentage of bacterial reduction was obtained; for Salmonella enterica it was reduced by 87% and 96.50%, Pseudomonas aeruginosa by 77.40% and 95.35% and Staphylococcus aureus by 87.60% and 98.73% respectively. It was found that the effect of ClO2 varied according to concentration and exposure time, i.e. the longer the exposure time the greater the reduction in bacterial growth compared to exposure times of 5min at concentrations of 5ppm where less bacterial reduction was obtained; for Salmonella enterica 17.7%, Pseudomonas aeruginosa 12.03% and Staphylococcus aureus 18.23%.
The present study aimed to evaluate the effect of chlorine dioxide (ClO2) on the growth of Salmonella enterica, Pseudomonas aeruginosa and Staphylococcus aureus under laboratory conditions. The tests were performed using ClO2 concentrations of 5ppm, 10ppm and 15ppm, with different exposure times of 5, 10 and 15 min, and were carried out in triplicate for each variable of time, concentration and bacterial strain. Bacterial reduction was determined by counting colony forming units (CFU) and calculating the percentage of reduction by arithmetic average of the results obtained. In addition, an ANOVA statistical analysis was performed to determine the significant differences in bacterial reduction between the different treatments. The results obtained revealed that chlorine dioxide showed a significant effect in the reduction of the growth of the three bacterial strains evaluated; being the concentrations of 10ppm, 15ppm and 15min of exposure where the highest percentage of bacterial reduction was obtained; for Salmonella enterica it was reduced by 87% and 96.50%, Pseudomonas aeruginosa by 77.40% and 95.35% and Staphylococcus aureus by 87.60% and 98.73% respectively. It was found that the effect of ClO2 varied according to concentration and exposure time, i.e. the longer the exposure time the greater the reduction in bacterial growth compared to exposure times of 5min at concentrations of 5ppm where less bacterial reduction was obtained; for Salmonella enterica 17.7%, Pseudomonas aeruginosa 12.03% and Staphylococcus aureus 18.23%.
Description
Keywords
dióxido de cloro, concentración de dióxido de cloro, tiempo de
exposición, desinfectante, porcentaje de reducción bacteriana, efecto
antimicrobiano.