Efecto antioxidante del extracto etanólico de Persea caerulea frente a estrés oxidativo inducido por acrilamida en hígado y cerebro de Mus musculus BALB/c
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Date
2022
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Universidad Nacional de Trujillo
Abstract
El presente trabajo de investigación estuvo orientado dilucidar el efecto antioxidante del extracto etanólico de Persea caerulea frente a estrés oxidativo (EO) inducido por acrilamida en hígado y cerebro de Mus Musculus BALB/c, para ello se trabajó con 25 ratones, distribuidos en 5 grupos, con los siguientes tratamientos: control negativo sin estrés oxidativo EO, tratado con solución salina fisiológica (SSF); control positivo, con EO inducido con una dosis única de acrilamida de 50 mg/Kg de peso corporal (pc), vía intraperitoneal y tratado con SSF; grupo Silimarina, con EO y tratado con 200 mg/Kg pc de silimarina; grupo experimental 50, con EO y tratado con 50 mg/Kg pc. de extracto de Persea caerulea, obtenida con el 96% de alcohol y grupo experimental 100, con EO y tratado con 100 mg/Kg pc. de extracto de Persea caerulea, el estrés oxidativo se determinó mediante la prueba de las sustancias reactivas al ácido tibarbitúrico (TBARS). Los resultados obtenidos fueron sometidos a una prueba ANOVA y Tukey, ambas con un 95% de confianza. Se concluye que la dosis de 100 mg/Kg pc del extracto etanólico de P. caerulea. tiene mayor efecto antioxidante en hígado y cerebro de Mus musculus.
The present research work was oriented to elucidate the antioxidant effect of the ethanolic extract of Persea caerulea against oxidative stress (OS) induced by acrylamide in liver and brain of Mus Musculus BALB/c. To this end, 25 mice were used, distributed in 5 groups, with the following treatments: negative control without oxidative stress EO, treated with physiological saline solution (PSS); positive control, with EO induced with a single dose of acrylamide of 50 mg/kg body weight (bw), intraperitoneally and treated with PSS; Silymarin group, with EO and treated with 200 mg/kg bw of silymarin; experimental group 50, with EO and treated with 50 mg/kg bw of Persea caerulea extract. of Persea caerulea extract, obtained with 96% alcohol and experimental group 100, with EO and treated with 100 mg/kg bw of Persea caerulea extract, oxidative stress was determined by the tibarbituric acid reactive substances (TBARS) test. The results obtained were subjected to ANOVA and Tukey test, both with 95% confidence. It is concluded that the dose of 100 mg/kg bw of the ethanolic extract of P. caerulea has a greater antioxidant effect on the liver and brain of Mus musculus.
The present research work was oriented to elucidate the antioxidant effect of the ethanolic extract of Persea caerulea against oxidative stress (OS) induced by acrylamide in liver and brain of Mus Musculus BALB/c. To this end, 25 mice were used, distributed in 5 groups, with the following treatments: negative control without oxidative stress EO, treated with physiological saline solution (PSS); positive control, with EO induced with a single dose of acrylamide of 50 mg/kg body weight (bw), intraperitoneally and treated with PSS; Silymarin group, with EO and treated with 200 mg/kg bw of silymarin; experimental group 50, with EO and treated with 50 mg/kg bw of Persea caerulea extract. of Persea caerulea extract, obtained with 96% alcohol and experimental group 100, with EO and treated with 100 mg/kg bw of Persea caerulea extract, oxidative stress was determined by the tibarbituric acid reactive substances (TBARS) test. The results obtained were subjected to ANOVA and Tukey test, both with 95% confidence. It is concluded that the dose of 100 mg/kg bw of the ethanolic extract of P. caerulea has a greater antioxidant effect on the liver and brain of Mus musculus.
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Efecto antioxidante