Capacidad fitorremediadora de Lemna minor “lenteja de agua” en agua contaminada con plomo
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Date
2022
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Universidad Nacional de Trujillo
Abstract
La presente tesis tuvo como objetivo determinar la capacidad fitorremediadora de Lemna minor “lenteja de agua” en agua contaminada con plomo. La macrófita fue recolectada en los canales de regadío de las chacras de arroz en el Centro Poblado Genaro Silva, Chepén, La Libertad. La especie fue identificada taxonómicamente como Lemna minuta Kunth por el Herbarium Tuxillense; ocasionando un cambio en el nombre de la muestra biológica. El lugar de trabajo fue el laboratorio S-308 de la Facultad de Ciencias Biológicas en la Universidad Nacional de Trujillo. La metodología se dividió en dos etapas: adaptación de la macrófita y aplicación de tratamientos. Se utilizaron doce recipientes de vidrio (2l), donde se colocaron 50g de planta y 1l de agua potable. En la primera etapa, se evaluó por tres días el pH y temperatura del agua, empleando un Multiparámetro HANNA Modelo HI 9829. En la segunda etapa, se expuso a las plantas por siete días a diferentes concentraciones de plomo; las cuales fueron 5, 10 y 20ppm, considerando un testigo. Se utilizaron cuatro tratamientos con tres repeticiones. Para calcular las concentraciones finales del metal se colectó 1l de agua de cada recipiente; las cuales fueron: 0.13ppm (tratamiento 2), 0.11ppm (tratamiento 3) y 0.20ppm (tratamiento 4). Los resultados fueron analizados estadísticamente mediante ANOVA, Prueba Tukey y el Programa Estadístico STATGRAPHICS Centurion XIX; y contrastados con los Estándares de Calidad Ambiental del Decreto Supremo Nº 004-2017 del Ministerio del Ambiente. Igualmente, se realizó un conteo aproximado de los cambios físicos de la macrófita, tomando como variables: especies vivas, muertas, cloróticas y necróticas.
The objective of this thesis was to determine the phytoremediation capacity of Lemna minor "duckweed" in water contaminated with lead. The macrophyte was collected in the irrigation canals of the rice fields in the Genaro Silva Populated Center, Chepén, La Libertad. The species was taxonomically identified as Lemna minuta Kunth by the Herbarium Tuxillense; causing a change in the name of the biological sample. The place of work was the S-308 laboratory of the Faculty of Biological Sciences at the National University of Trujillo. The methodology was divided into two stages: adaptation of the macrophyte and application of treatments. Twelve glass containers (2l) were used, where 50g of plant and 1l of drinking water were placed. In the first stage, the pH and temperature of the water were evaluated for three days, using a HANNA Model HI 9829 Multiparameter. In the second stage, the plants were exposed for seven days to different concentrations of lead; which were 5, 10 and 20ppm, considering a witness. Four treatments with three repetitions were used. To calculate the final concentrations of the metal, 1l of water was collected from each container; which were: 0.13ppm (treatment 2), 0.11ppm (treatment 3) and 0.20ppm (treatment 4). The results were statistically analyzed using ANOVA, Tukey Test and the STATGRAPHICS Centurion XIX Statistical Program; and contrasted with the Environmental Quality Standards (ECA) of Supreme Decree No. 004-2017 of the Ministry of the Environment (MINAM). Likewise, an approximate count of the physical changes of the macrophyte was made, taking as variables: living, dead, chlorotic and necrotic species.
The objective of this thesis was to determine the phytoremediation capacity of Lemna minor "duckweed" in water contaminated with lead. The macrophyte was collected in the irrigation canals of the rice fields in the Genaro Silva Populated Center, Chepén, La Libertad. The species was taxonomically identified as Lemna minuta Kunth by the Herbarium Tuxillense; causing a change in the name of the biological sample. The place of work was the S-308 laboratory of the Faculty of Biological Sciences at the National University of Trujillo. The methodology was divided into two stages: adaptation of the macrophyte and application of treatments. Twelve glass containers (2l) were used, where 50g of plant and 1l of drinking water were placed. In the first stage, the pH and temperature of the water were evaluated for three days, using a HANNA Model HI 9829 Multiparameter. In the second stage, the plants were exposed for seven days to different concentrations of lead; which were 5, 10 and 20ppm, considering a witness. Four treatments with three repetitions were used. To calculate the final concentrations of the metal, 1l of water was collected from each container; which were: 0.13ppm (treatment 2), 0.11ppm (treatment 3) and 0.20ppm (treatment 4). The results were statistically analyzed using ANOVA, Tukey Test and the STATGRAPHICS Centurion XIX Statistical Program; and contrasted with the Environmental Quality Standards (ECA) of Supreme Decree No. 004-2017 of the Ministry of the Environment (MINAM). Likewise, an approximate count of the physical changes of the macrophyte was made, taking as variables: living, dead, chlorotic and necrotic species.
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Keywords
fitorremediación, plomo, agua contaminada