Efecto del extracto acuoso liofilizado de Trifolium pratense L. sobre depresión inducida en Rattus norvegicus Holtzman
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente trabajo tuvo como objetivos determinar el efecto de Trifolium pratense L. sobre los parámetros de nado horizontal (activación serotoninérgica), nado vertical (activación noradrenérgica) e inmovilidad según test de Porsolt en Rattus norvegicus Holtzman. La muestra de Trifolium pratense L., fue recolectada del distrito de Chota, provincia de Chota, región de Cajamarca, Perú, se llevó a una pre-congelación de -80 °C y posteriormente se llevó al proceso de liofilización. Se usaron 24 especímenes hembras de Rattus norvegicus Holtzman, distribuidos en 4 grupos, control (agua destilada), patrón (fluoxetina 20 mg/kg) y 2 grupos problema (extracto acuoso liofilizado de Trifolium pratense L. de 250 mg/kg y 500 mg/kg), por 28 días. Se utilizó el método de la prueba de nado forzado modificado (Test de Porsolt modificado) para determinación de la actividad antidepresiva. Las variables que se evaluaron fueron nado vertical, nado horizontal e inmovilidad, durante 5 minutos en el día 28 del tratamiento. Los datos se analizaron a través de la prueba de ANOVA y test de Bonferroni como prueba post hoc. Los resultados evidenciaron que las dosis de 250 y 500 mg/kg del extracto acuoso liofilizado de Trifolium pratense L. en nado horizontal (swimming) presentaron efecto antidepresivo estadísticamente similar (p > 0,05) al patrón (fluoxetina) señalando la activación serotoninérgica, y en el parámetro de inmovilidad se generó un efecto decreciente (p < 0.001), evidenciando como tal el efecto antidepresivo esperado.
ABSTRACT The aim of this study was to determine the effect of Trifolium pratense L. on the parameters of horizontal swimming (serotonergic activation), vertical swimming (noradrenergic activation) and immobility according to the Porsolt test in Rattus norvegicus Holtzman. The sample of Trifolium pratense L., collected from the district of Chota, province of Chota, Cajamarca region, Peru, was pre-frozen at -80 °C and then taken to the freeze-drying process. Twenty-four female specimens of Rattus norvegicus Holtzman were used, distributed in four groups, control (distilled water), standard (fluoxetine 20 mg/kg) and two problem groups (freeze-dried aqueous extract of Trifolium pratense L. at 250 mg/kg and 500 mg/kg), for 28 days. The modified forced swim test method (modified Porsolt test) was used to determine the antidepressant activity. The variables assessed were vertical swim, horizontal swim and immobility for 5 minutes on day 28 of treatment. Data were analysed using ANOVA and Bonferroni's test as a post hoc test. The results showed that the doses of 250 and 500 mg/kg of the freeze-dried aqueous extract of Trifolium pratense L. in horizontal swimming (swimming) presented an antidepressant effect statistically similar (p > 0.05) to the standard (fluoxetine) indicating serotonergic activation, and in the immobility parameter a decreasing effect was generated (p < 0.001), evidencing as such the expected antidepressant effect.
ABSTRACT The aim of this study was to determine the effect of Trifolium pratense L. on the parameters of horizontal swimming (serotonergic activation), vertical swimming (noradrenergic activation) and immobility according to the Porsolt test in Rattus norvegicus Holtzman. The sample of Trifolium pratense L., collected from the district of Chota, province of Chota, Cajamarca region, Peru, was pre-frozen at -80 °C and then taken to the freeze-drying process. Twenty-four female specimens of Rattus norvegicus Holtzman were used, distributed in four groups, control (distilled water), standard (fluoxetine 20 mg/kg) and two problem groups (freeze-dried aqueous extract of Trifolium pratense L. at 250 mg/kg and 500 mg/kg), for 28 days. The modified forced swim test method (modified Porsolt test) was used to determine the antidepressant activity. The variables assessed were vertical swim, horizontal swim and immobility for 5 minutes on day 28 of treatment. Data were analysed using ANOVA and Bonferroni's test as a post hoc test. The results showed that the doses of 250 and 500 mg/kg of the freeze-dried aqueous extract of Trifolium pratense L. in horizontal swimming (swimming) presented an antidepressant effect statistically similar (p > 0.05) to the standard (fluoxetine) indicating serotonergic activation, and in the immobility parameter a decreasing effect was generated (p < 0.001), evidencing as such the expected antidepressant effect.
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Keywords
Nado forzado modificado. Depresión. Trifolium pratense L.