Identificación de rutina por cromatografía líquida acoplada a espectrometría de masas
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Date
2022
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Universidad Nacional de Trujillo
Abstract
El objetivo es caracterizar rutina por LC-MS/MS. El estándar rutina (ST), rutina aislada de la “flor de overo” (RACL) y extracto metanólico de las flores de Cordia lutea (EMCL) fueron los objetos de estudio. El estándar sirvió como objeto de comparación con las otras muestras, cada una de ellas fueron analizadas por un equipo de UHPLC-ESI-MS/MS. Se utilizaron las fases móviles de acetonitrilo y agua, acidificados con ácido fórmico, y la fase estacionaria C18. Se evaluaron los perfiles cromatográficos del estándar, aislado y extracto metanólico de “flor de overo” reportados a 254 nm, presentando el pico de rutina en los tR de 11,03; 11,02 y 11,03 min respectivamente. En ese mismo tiempo se observaron los picos de rutina en los espectros de masas, el cual por M1 presento el ion precursor de 609 m/z y 611 m/z por modo positivo y negativo respectivamente. El espectro por M2 en negativo se observó los fragmentos de 151, 179, 271, 301 y 343 m/z y en positivo 129, 147, 303 y 465 m/z. Tanto los iones precursores como los fragmentos fueron iguales en los tres espectros de masas. Se caracterizó la rutina por LC-MS/MS en ambos modos de ionización.
ABSTRACT The objetive is to characterize rutin by LC-MS/MS. Rutin standard (ST), rutin isolated from “flor de overo” and methanolic extract from the flowers of Cordia lutea were the objects of study. The standard served as the object of comparison with the other samples, each of which was analyzed by a UHPLC-ESI-MS/MS equipment. The mobile phases of acetonitrile and water, acidified with formic acid, and the stationary phase C18 were used. The chromatographic profiles of the standard, isolate and methanolic extract of “flor de overo” reported at 254 nm were evaluated, presenting the rutin peak in the tR of 11.03; 11.02 and 11.03 min respectively. At that same time, the routine peaks were observed in the mass spectra, which for M1 presented the precursor ion of 609 m/z and 611 m/z by positive and negative mode, respectively. The spectrum for M2 in negative was observed the fragments of 151, 179, 271, 301 and 343 m/z and in positive 129, 147, 303 and 465 m/z. Both the precursor ions and the fragments were the same in all three mass spectra. Rutin was characterized by LC-MS/MS in both ionization modes.
ABSTRACT The objetive is to characterize rutin by LC-MS/MS. Rutin standard (ST), rutin isolated from “flor de overo” and methanolic extract from the flowers of Cordia lutea were the objects of study. The standard served as the object of comparison with the other samples, each of which was analyzed by a UHPLC-ESI-MS/MS equipment. The mobile phases of acetonitrile and water, acidified with formic acid, and the stationary phase C18 were used. The chromatographic profiles of the standard, isolate and methanolic extract of “flor de overo” reported at 254 nm were evaluated, presenting the rutin peak in the tR of 11.03; 11.02 and 11.03 min respectively. At that same time, the routine peaks were observed in the mass spectra, which for M1 presented the precursor ion of 609 m/z and 611 m/z by positive and negative mode, respectively. The spectrum for M2 in negative was observed the fragments of 151, 179, 271, 301 and 343 m/z and in positive 129, 147, 303 and 465 m/z. Both the precursor ions and the fragments were the same in all three mass spectra. Rutin was characterized by LC-MS/MS in both ionization modes.
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Keywords
Cromatografía, Espectrometría