Detección de Pseudomonas aeruginosa productora de Serina-beta-lactamasas y/o Metalo-beta-lactamasas, en cultivos de origen clínico, Trujillo - Perú, 2022
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Date
2024
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Publisher
Universidad Nacional de Trujillo
Abstract
El presente trabajo tuvo como objetivo determinar el porcentaje de cultivos de Pseudomonas aeruginosa productora de Serina-beta-lactamasas (SBL) y/o Metalo-beta-lactamasa (MBL) mediante pruebas fenotípicas, en cultivos resistentes a carbapenémicos, proporcionados por dos hospitales ubicados en la provincia de Trujillo – Perú, en el periodo enero - mayo del 2022. Por ello, se recolectaron 82 cultivos bacterianos, 48 del Hospital de Alta Complejidad de La Libertad “Virgen de La Puerta” (HVP) y 34 del “Hospital Belén” De Trujillo (HB), a los cuales se les evaluó si eran productores de carbapenemasas mediante métodos fenotípicos como el Método de inactivación de carbapenémico modificado (MICm) y la Prueba de discos combinados (PDC). Mediante MICm se detectaron 16 cultivos productores de carbapenemasas. Y mediante PDC se detectaron 13 cultivos del HB, como productores de MBL y 0 cultivos productor de SBL; también se obtuvieron 3 (2 del HB y 1 del HVP) cultivos indeterminados. En conclusión, se detectó 16 cultivos de P. aeruginosa productores de carbapenemasas, de ellos, 15.85% fenotípicamente productores de MBL, 0% SBL y 0% de la coproducción MBL/SBL, proporcionados por dos hospitales ubicados en la provincia de Trujillo – Perú, en el periodo enero - mayo del 2022.
The objective of this work was to determine the percentage of cultures of Pseudomonas aeruginosa producing Serine-beta-lactamases (SBL) and/or Metallo-beta-lactamases (MBL) through phenotypic tests, in cultures resistant to carbapenems, provided by two hospitals located in the province of Trujillo - Peru, in the period January - May 2022. Therefore, 82 cultures were collected, 48 from the Hospital de Alta Complejidad de La Libertad “Virgen de La Puerta” (HVP) and 34 from the “Hospital Belén'' de Trujillo (HB), which were evaluated if they were carbapenemase producers using phenotypic methods such as the Modified Carbapenem Inactivation Method (CIMm) and the Combined Disc Test (CDT). Using MICm, 16 carbapenemases-producing cultures were detected. And through CDT, 13 HB cultures were detected as producers of MBL and 0 cultures as producers of SBL; 3 (2 from HB and 1 from HVP) indeterminate cultures were also obtained. In conclusion, 16 cultures of P. aeruginosa producing carbapenemases were detected, of them, 15.85% phenotypically producing MBL, 0% SBL and 0% of the MBL/SBL co-production, provided by two hospitals located in the province of Trujillo – Peru, in the period January - May 2022.
The objective of this work was to determine the percentage of cultures of Pseudomonas aeruginosa producing Serine-beta-lactamases (SBL) and/or Metallo-beta-lactamases (MBL) through phenotypic tests, in cultures resistant to carbapenems, provided by two hospitals located in the province of Trujillo - Peru, in the period January - May 2022. Therefore, 82 cultures were collected, 48 from the Hospital de Alta Complejidad de La Libertad “Virgen de La Puerta” (HVP) and 34 from the “Hospital Belén'' de Trujillo (HB), which were evaluated if they were carbapenemase producers using phenotypic methods such as the Modified Carbapenem Inactivation Method (CIMm) and the Combined Disc Test (CDT). Using MICm, 16 carbapenemases-producing cultures were detected. And through CDT, 13 HB cultures were detected as producers of MBL and 0 cultures as producers of SBL; 3 (2 from HB and 1 from HVP) indeterminate cultures were also obtained. In conclusion, 16 cultures of P. aeruginosa producing carbapenemases were detected, of them, 15.85% phenotypically producing MBL, 0% SBL and 0% of the MBL/SBL co-production, provided by two hospitals located in the province of Trujillo – Peru, in the period January - May 2022.
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Keywords
Pseudomonas aeruginosa, Carbapenemasas, Serina-beta-lactamasas, Metalo-beta-lactamasa