Estudio fitoquímico de las hojas de artemisia absinthium y su actividad antimalárica
Sagastegui Guarniz, William Antonio
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The present work was designed to determine the phytochemical components of the leaves of Artemisia absinthium and evaluate the effect in vitro of each crop of Plasmodium vivax and P. falciparum, the causative agents of malaria in Peru to help with new active substances for the treatment and alternative malaria control. The effect in vivo was performed on specimens previously infected Aotus nancimae crops Plasmodium falciparum. Samples were collected from plants of Artemisia absinthium, family Asteraceae, "ajenjo" Contumazá Province, Cajamarca Department, its leaves were dried, pulverized and extracted with solvents: hexane, chloroform, ethyl acetate, methanol and water; in these extracts was identified by color and precipitation reactions following fitoconstituyentes: alkaloids, anthraquinones and terpenes, terpenoids, flavonoids, lactones, tannins, sterols, and coumarins. Then the extracts were diluted doses of 10 and 100 µg/mL and administered to cultures Plasmodium vivax and P. falciparum, and parasitemia was assessed after 72 h of incubation, found a significant decrease in the percentage of parasitemia. The percentage inhibition antiplasmodium was good to excellent, with extracts of ethyl acetate and methanol which had a higher percentage of inhibition of P. vivax, 92.0562% and 94.5840%, respectively, and for P. falciparum malaria was 89.9146 and 91.5607% inhibition, compared with the control group than normal development of parasitemia. For testing in vivo dose was prepared from 50.0 mg/mL and was administered orally e intramuscular to Aotus nancimae specimens previously infected with strains of Plamodium falciparum, being 75% effective in each case, which to be similar to control group that was administered to 10.0 mg sodium artesunate.