Determinación de nematodos formadores de quistes de la subfamilia Heteroderinae que afectan a Solanum tuberosum L. en Carabamba, Julcán, La Libertad
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue determinar los géneros de nematodos formadores de quistes de la subfamilia Heteroderinae presentes en las muestras de suelo y raíces de campos productores de papa de los caseríos San Isidro, San Fernando, Santa Rosa, Munday Muchiquil, Carabamba y Túpac Amaru del distrito de Carabamba, en Julcán, La Libertad. El suelo fue tamizado y puesto a secar a temperatura ambiente por 10 días; luego se procedió extraer y contar el número promedio de quistes en 100 cc de suelo, así como. el número promedio de quistes en 5 gramos de raíces. Posteriormente, se tomó al azar 10 quistes por muestra y se procedió a realizar cortes de la región perineal para determinar número de estrías, distancia entre vulva y ano, diámetro de fenestra y el radio de Graneck. De igual forma en los juveniles de segundo estadio se midió la longitud del cuerpo, longitud de cola, tamaño y forma de los nódulos basales del estile. El 85.7% de las muestras presentó quistes en un rango de 18 a 1056 por 100 cc de suelo y de 2 a 215 en 5 gramos de raíces. En base a las características de la región perineal, los nematodos formadores de quistes identificados en la zona de estudio correspondieron el 91% a Globodera pallida y el 9% a Globodera rostochiensis.
ABSTRACT The objective of this research was to determine the genera of cyst-forming nematodes of the subfamily Heteroderinae present in soil and root samples from potato-producing fields in the San Isidro, San Fernando, Santa Rosa, Munday Muchiquil, Carabamba and Tupac Amaru hamlets from the Carabamba district, in Julcán, La Libertad. The soil was sieved and dried at room temperature for 10 days; then, the average number of cysts in 100 cc of soil was extracted and counted, as well as. the average number of cysts in 5 grams of roots. Subsequently, 10 cysts per sample were taken at random and sections were made from the perineal region to determine the number of striae, distance between vulva and anus, fenestra diameter and Graneck's radius. Similarly, in second instar juveniles, body length, tail length, size and shape of the basal nodes of the style were measured. The results show 85.7% of de samples presented cysts in a range of 18 to 1056 per 100 cc of soil and 2 to 215 in 5 grams of roots. Based on the characteristics of the perineal region, 91% of the cyst-forming nematodes identified in the study area corresponded to Globodera pallida and 9% to Globodera rostochiensis.
ABSTRACT The objective of this research was to determine the genera of cyst-forming nematodes of the subfamily Heteroderinae present in soil and root samples from potato-producing fields in the San Isidro, San Fernando, Santa Rosa, Munday Muchiquil, Carabamba and Tupac Amaru hamlets from the Carabamba district, in Julcán, La Libertad. The soil was sieved and dried at room temperature for 10 days; then, the average number of cysts in 100 cc of soil was extracted and counted, as well as. the average number of cysts in 5 grams of roots. Subsequently, 10 cysts per sample were taken at random and sections were made from the perineal region to determine the number of striae, distance between vulva and anus, fenestra diameter and Graneck's radius. Similarly, in second instar juveniles, body length, tail length, size and shape of the basal nodes of the style were measured. The results show 85.7% of de samples presented cysts in a range of 18 to 1056 per 100 cc of soil and 2 to 215 in 5 grams of roots. Based on the characteristics of the perineal region, 91% of the cyst-forming nematodes identified in the study area corresponded to Globodera pallida and 9% to Globodera rostochiensis.
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Keywords
Radio de Graneck, Nematodo quiste, Región perineal, Estomatoestilete, Solanum tuberosum L.