Tesis de Estomatología
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Browsing Tesis de Estomatología by Subject "Aloe vera"
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Item “Comparación in vitro del efecto antibacteriano de los extractos etanólicos de Aloe vera (L) y Curcuma longa L. frente a Streptococcus mutans ATCC 25175”(Universidad Nacional de Trujillo, 2022) Rios Vonderheyde, Sara Antonieta; Guardia Méndez, Gustavo; Zavaleta Espejo, Gina GenaraThe purpose of this experimental research "in vitro" was to compare the antibacterial effect of the ethanolic extract of Aloe vera (L) and Curcuma longa L., on the growth of Streptococcus mutans ATCC 25175. Ethanolic extracts of Aloe vera ( L) and Curcuma longa L. at concentrations of 25%, 50% and 75% each. The Streptococcus mutans strain ATCC 25175 was reactivated in brain heart broth. To evaluate the antibacterial effect, 10 repetitions of each concentration were carried out using the Kirby Bauer method, making use of filter paper disks placed in plates containing Müeller Hinton Agar-blood inoculated with S. mutans. Chlorhexidine 0.12% was used as a positive control and distilled water as a negative control. The diameters in (mm) of the inhibition halos around each disc were measured with a vernier pachymeter. The ethanolic extract of Curcuma longa L. did not present inhibition halos for the concentrations of 25% and 50%, but it did present an inhibition halo of 8.2 mm for the 75% concentration. The ethanolic extract of Aloe vera (L) presented an inhibition halo of 8.4 mm for the 25% concentration, 9.5 mm for the 50% concentration and 11.7 mm for the 75% concentration. Chlorhexidine 0.12% presented an inhibition halo of 25.2 mm while the negative control did not present an inhibition halo. The ANOVA test showed that there is a statistically significant difference between the concentrations of the extracts. When comparing the results of both extracts, it is concluded that the ethanolic extract Aloe vera (L) at 75% presented the greatest antibacterial effect against S. mutans.Item Efecto antimicrobiano in vitro del extracto etanólico hoja Aloe vera sobre Streptoccocus mutans ATCC 25175(Universidad Nacional de Trujillo, 2020) Lecca Rojas, Roberto Manuel; Guardia Méndez, Gustavon the present study of the experimental type, it was intended to determine the in vitro antibacterial effect of the ethanolic extract of the Aloe vera leaf (Aloe Vera) against Streptococcus mutans. The susceptibility test was performed, to determine the minimum bactericidal concentration (CMB) the diameter of the inhibition halos formed in the Petri dishes was measured over the concentrations of 25%, 50% and 75% of the ethanolic extract of the Aloe leaf Vera and his control. All presented halos of inhibition in which there was proportionality (The diameter of the halo of inhibition is directly proportional to the concentrations used) To determine the Minimum Inhibitory Concentration, the tube dilution method was used, having the same concentrations and their control, each culture was seeded with Mueller Hinton-Blood Agar to determine the Colony Forming Units (CFUs). The results obtained for the Minimum Bactericidal Concentration (CMB) by measuring the inhibition halos when 25%, 50% and 75% of the ethanol extract of Aloe vera were used were 7.6 mm, 10.7 mm and 13.5 mm respectively; and in the 45mm control group The results obtained for Minimum Inhibitory Concentration (MIC) measuring the turbidity of the tube and inoculating in Petri dishes with Mueller Hinton-Blood agar, 25% colony growth of 0.9x102 CFU and not 50% and 75 %, the negative control group 2.8x105UFC´s and the positive control group or UFC´s. Finally, it is concluded that the ethanolic extract of the Aloe vera leaf does have in vitro antimicrobial activity on Streptococcus mutans.