Efecto antibacteriano in vitro de extractos etanólicos de cuminum cyminum sobre streptococcus mutans atcc 25175
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Date
2016
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Universidad Nacional de Trujillo
Abstract
El propósito de este estudio de tipo experimental, fue el determinar el efecto antibacteriano in vitro de extractos etanólicos del Cuminum cyminum (comino) sobre Streptococcus mutans ATC 25175. Se realizó la prueba de susceptibilidad, utilizando el método de difusión de discos ensayada sobre las concentraciones de 5%, 10% y 20% puras (al 100%) con sus respectivas diluciones al 25%, 50% y 75% de extracto etanólico de Cuminum cyminum además del control con Etanol de 50º Para hallar la Concentración Mínima Inhibitoria se empleó el método de dilución de tubos, ensayando las mismas concentraciones con sus respectivas diluciones y su control; de cada cultivo se sembró placas con Agar Mueller Hinton - Sangre para determinar las Unidades Formadoras de Colonias (UFC). Los resultados mostraron que la concentración al 20% pura del extracto etanólico de Cuminum cyminum (comino) mostró la mayor susceptibilidad frente a la cepa de Streptococcus mutans ATCC 25175 y la concentración mínima inhibitoria fue la de 20% a la dilución de 25% en donde no se visualizó crecimiento de colonias en todas las repeticiones de la muestra ensayada. Finalmente se concluye que los extracto etanólico de Cuminum cyminum (comino) poseen actividad antibacteriana in vitro sobre el crecimiento de cepas de Streptococcus mutans.
The purpose of this experimental study was to determine the antibacterial effect in vitro of ethanolic extracts of Cuminum cyminum (cumin) on Streptococcus mutans ATC 25175. The susceptibility test was performed using the disc diffusion method tested on concentrations of 5%, 10% and 20% pure (to 100%) with their respective dilutions to 25%, 50% and 75% of ethanolic extract of Cuminum cyminum in addition to the control with Ethanol of 50º. To find the Minimum Inhibitory Concentration the tube dilution method was used, testing the same concentrations with their respective dilutions and their control; of each culture plates were plated with Mueller Hinton - Blood Agar to determine the Colony Forming Units (CFU). The results showed that the 20% pure concentration of the ethanol extract of Cuminum cyminum (cumin) showed the highest susceptibility to the Streptococcus mutans ATCC 25175 strain and the minimum inhibitory concentration was 20% at the 25% dilution where colony growth was not visualized in all replicates of the sample tested. Finally it is concluded that the ethanolic extract of Cuminum cyminum (cumin) possess antibacterial activity in vitro on the growth of strains of Streptococcus mutans.
The purpose of this experimental study was to determine the antibacterial effect in vitro of ethanolic extracts of Cuminum cyminum (cumin) on Streptococcus mutans ATC 25175. The susceptibility test was performed using the disc diffusion method tested on concentrations of 5%, 10% and 20% pure (to 100%) with their respective dilutions to 25%, 50% and 75% of ethanolic extract of Cuminum cyminum in addition to the control with Ethanol of 50º. To find the Minimum Inhibitory Concentration the tube dilution method was used, testing the same concentrations with their respective dilutions and their control; of each culture plates were plated with Mueller Hinton - Blood Agar to determine the Colony Forming Units (CFU). The results showed that the 20% pure concentration of the ethanol extract of Cuminum cyminum (cumin) showed the highest susceptibility to the Streptococcus mutans ATCC 25175 strain and the minimum inhibitory concentration was 20% at the 25% dilution where colony growth was not visualized in all replicates of the sample tested. Finally it is concluded that the ethanolic extract of Cuminum cyminum (cumin) possess antibacterial activity in vitro on the growth of strains of Streptococcus mutans.
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Keywords
In vitro, Efecto antibacteriano, Comino, Streptococcus mutans