Incremento de biomasa y producción de metabolitos secundarios en callos inducidos a partir de tallos de Stevia rebaudiana vars. criolla y eirete
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Date
2023
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Universidad Nacional de Trujillo
Abstract
Se demostró diferencias significativas en el aumento de biomasa e incremento en la producción de metabolitos secundarios con explantes de Stevia rebaudiana var Criolla y Eirete que fueron cultivadas en distintos tratamientos de medios basales (“A”, “B”, “C”). El crecimiento de biomasa se verificó comparando los pesos semanalmente de los callos, el cual, se detuvo en la semana 7 para posteriormente iniciar su proceso de necrosis. En el caso de los metabolitos secundarios, se obtuvo las concentraciones de flavonoides y fenoles usando las curvas de calibración y los resultados de espectrofotometría de cada tratamiento a distintas longitudes de onda. Las diferencias significativas resultantes de las concentraciones de los metabolitos, demuestran la eficiencia de los tratamientos de auxinas y citoquininas.
Significant differences were concluded in the increase in biomass and increase in the production of secondary metabolites with explants of Stevia rebaudiana var.Criolla and Eirete that were cultivated in different basal media treatments (“A”, “B”, “C”). The biomass growth was verified by cimparing the weekly wightd of the calluses, wich stopped at week 7 to later start the necrosis process. In the case of secondary metabolites, the concentrations of flavonoids and phenols were obtained using the calibration curves and the spectrophotometry results of each treatment at different wavelengths. The significant differences resulted from the concentrations of the metabolites, demonstrating the efficiency of the auxin and cytokinin trearments.
Significant differences were concluded in the increase in biomass and increase in the production of secondary metabolites with explants of Stevia rebaudiana var.Criolla and Eirete that were cultivated in different basal media treatments (“A”, “B”, “C”). The biomass growth was verified by cimparing the weekly wightd of the calluses, wich stopped at week 7 to later start the necrosis process. In the case of secondary metabolites, the concentrations of flavonoids and phenols were obtained using the calibration curves and the spectrophotometry results of each treatment at different wavelengths. The significant differences resulted from the concentrations of the metabolites, demonstrating the efficiency of the auxin and cytokinin trearments.
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Keywords
metabolito secundario, flavonoides, fenoles, fitohormonas.