Composición química del aceite esencial de hojas de Foeniculum vulgare Mill y su efecto ansiolítico en ratones Mus musculus BALB/c
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Este estudio tuvo como objetivo determinar la composición química del aceite esencial de hojas de Foeniculum vulgare Mill y evaluar su efecto ansiolítico en ratones Mus musculus Balb/c. El aceite esencial se obtuvo aplicando destilación con vapor de agua, una técnica que aísla los compuestos volátiles presentes. Luego, su composición fue examinada mediante GC-MS, un método que permite identificar y caracterizar cada componente con alta exactitud. Los parámetros organolépticos y fisicoquímicos del aceite esencial se encontraron dentro de los límites establecidos en las farmacopeas, con un rendimiento del 0,5±0,01 % v/p. Mediante el análisis por GC-MS se identificaron 23 compuestos químicos, siendo los principales trans- Anetol, α-pineno, α-limoneno. El aceite esencial de Foeniculum vulgare Mill, administrado en dosis de 12,5 mg/kg y 25 mg/kg, mostraron un efecto ansiolítico significativo en el modelo de Luz/Oscuridad; siendo la dosis de 25 mg/kg la que mostró un efecto ansiolítico similar al diazepam (1 mg/kg) con un p>0.05.
ABSTRACT This study aimed to determine the chemical composition of the essential oil from Foeniculum vulgare Mill leaves and evaluate its anxiolytic effect in Mus musculus Balb/c mice. The essential oil was obtained using steam distillation, a technique that isolates volatile compounds. Its composition was then analyzed using GC-MS, a method that enables the identification and characterization of each component with high accuracy. The organoleptic and physicochemical parameters of the essential oil were found to be within the limits established by pharmacopeias, with a yield of 0.5±0.01% v/w. GC-MS analysis identified 23 chemical compounds, with the main ones being trans-anethole, α-pinene, and α-limonene. The essential oil of Foeniculum vulgare Mill, administered at doses of 12.5 mg/kg and 25 mg/kg, showed a significant anxiolytic effect in the Light/Dark model, with the 25 mg/kg dose demonstrating an anxiolytic effect comparable to diazepam (1 mg/kg) with p>0.05.
ABSTRACT This study aimed to determine the chemical composition of the essential oil from Foeniculum vulgare Mill leaves and evaluate its anxiolytic effect in Mus musculus Balb/c mice. The essential oil was obtained using steam distillation, a technique that isolates volatile compounds. Its composition was then analyzed using GC-MS, a method that enables the identification and characterization of each component with high accuracy. The organoleptic and physicochemical parameters of the essential oil were found to be within the limits established by pharmacopeias, with a yield of 0.5±0.01% v/w. GC-MS analysis identified 23 chemical compounds, with the main ones being trans-anethole, α-pinene, and α-limonene. The essential oil of Foeniculum vulgare Mill, administered at doses of 12.5 mg/kg and 25 mg/kg, showed a significant anxiolytic effect in the Light/Dark model, with the 25 mg/kg dose demonstrating an anxiolytic effect comparable to diazepam (1 mg/kg) with p>0.05.
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Keywords
Foeniculum vulgare Mill. Aceite esencial. Ansiedad. Mus musculus BALB/c