Control fisicoquímico y microbiológico del agua usada en la fabricación de gasas estériles en un laboratorio nacional 2022
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Date
2022
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Universidad Nacional de Trujillo
Abstract
Este presente informe de Prácticas Pre-Profesionales se desarrolló en un laboratorio nacional y su principal objetivo fue ejecutar el control de calidad del agua purificada usada en la fabricación de gasas estériles. Para poder alcanzar este objetivo se realizó dos tipos de ensayos estandarizados según normatividad vigente: controles fisicoquímicos (Aspecto, pH, Conductividad, Cloro libre y Determinación Cualitativa de Sulfatos) y controles microbiológicos (Recuento de Heterótrofos por siembra incorporado, Detección de coliformes totales y Pseudomonas Aeruginosa), por lo que se basó en las especificaciones establecidas por The United States Pharmacopeia (USP-42) y el DS N°031-2010-SA denominada “Reglamento de Calidad del Agua para consumo humano” tanto para agua potable como agua purificada. De acuerdo a los resultados promedios finales obtenidos en un periodo trimestral, se determinó para el control fisicoquímico de agua purificada un aspecto transparente, incoloro e inodoro; un pH promedio 6,23; conductividad promedio 1,04 µS/cm; presencia de cloro libre promedio menor a 0,1 y ausencia cualitativa de sulfatos. En cuanto a los controles microbiológicos para el agua purificada se cumplió con las especificaciones tanto para Recuento de Heterótrofos con un valor promedio <1 UFC/mL como la ausencia de Coliformes Totales y Pseudomonas Aeruginosa. Finalmente, se concluyó que el agua purificada usada en los procesos de producción de gasas estériles si cumplen con las especificaciones técnicas internacionales y nacionales vigentes.
ABSTRACT This present Pre-Professional Practices report was developed in a national laboratory and its main objective was to carry out quality control of the purified water used in the manufacture of sterile gauzes. In order to achieve this objective, two types of standardized tests were carried out according to current regulations: physicochemical controls (Aspect, pH, Conductivity, Free Chlorine and Qualitative Determination of Sulphates) and microbiological controls (Heterotroph Count by planting incorporated, Detection of total coliforms and Pseudomonas aeruginosa), so it was based on the specifications established by The United States Pharmacopeia (USP-42) and Supreme Decree N°. 031-2010-SA called "Water Quality Regulations for human consumption" for both drinking water and purified water. According to the final average results obtained in a quarterly period, it was determined for the physicochemical control of purified water that it had a transparent, colorless and odorless appearance; an average pH 6,23; average conductivity 1,04 µS/cm; presence of average free chlorine less than 0,1 and qualitative absence of sulfates. Regarding the microbiological controls for the purified water, the specifications were met for both the Heterotroph Count with an average value <1 CFU/mL and the absence of Total Coliforms and Pseudomonas Aeruginosa. Finally, it was concluded that the purified water used in the production processes of sterile gauzes does comply with current international and national technical specifications.
ABSTRACT This present Pre-Professional Practices report was developed in a national laboratory and its main objective was to carry out quality control of the purified water used in the manufacture of sterile gauzes. In order to achieve this objective, two types of standardized tests were carried out according to current regulations: physicochemical controls (Aspect, pH, Conductivity, Free Chlorine and Qualitative Determination of Sulphates) and microbiological controls (Heterotroph Count by planting incorporated, Detection of total coliforms and Pseudomonas aeruginosa), so it was based on the specifications established by The United States Pharmacopeia (USP-42) and Supreme Decree N°. 031-2010-SA called "Water Quality Regulations for human consumption" for both drinking water and purified water. According to the final average results obtained in a quarterly period, it was determined for the physicochemical control of purified water that it had a transparent, colorless and odorless appearance; an average pH 6,23; average conductivity 1,04 µS/cm; presence of average free chlorine less than 0,1 and qualitative absence of sulfates. Regarding the microbiological controls for the purified water, the specifications were met for both the Heterotroph Count with an average value <1 CFU/mL and the absence of Total Coliforms and Pseudomonas Aeruginosa. Finally, it was concluded that the purified water used in the production processes of sterile gauzes does comply with current international and national technical specifications.
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Keywords
Agua. Calidad. Especificaciones. pH. Conductividad