Efecto in vitro del extracto hidroalcohólico de Portulaca oleracea L. sobre el crecimiento de Staphylococcus aureus resistente a la meticilina
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Date
2023
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Universidad Nacional de Trujillo
Abstract
En el presente estudio se determinó el efecto in vitro del extracto hidroalcohólico de Portulaca oleracea L. a 0.625, 1.25, 2.5 y 5% sobre el crecimiento de Staphylococcus aureus resistente a la meticilina. Para esta evaluación se aplicaron cinco grupos de estudio, un grupo blanco y cuatro experimentales, con los que se evaluó el efecto del extracto hidroalcohólico de P. oleracea L. mediante la técnica de difusión en pocillos, la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB). Se
identificaron cualitativamente los fitoconstituyentes del extracto y su fitotoxicidad in vivo mediante el porcentaje de mortalidad sobre nauplios de Artemia salina. Se encontró que el incremento de la concentración del extracto en estudio generó halos de inhibición de 13.07 ± 0.88, 17.33 ± 1.23, 24.40 ± 1.18 y 26.87 ± 1.41 mm de diámetro; una CMI de 12.5 mg/mL y una CMB de 25 mg/mL. Los fitoconstituyentes identificados cualitativamente fueron compuestos fenólicos, flavonoides, esteroides y alcaloides, y la toxicidad sobre nauplios de Artemia salina fue elevada. El análisis estadístico muestra que existe diferencia significativa (p < 0.01) entre el grupo blanco y experimentales; así como también, entre los grupos experimentales a las diferentes concentraciones en estudio; la relación CMB/CMI indica que el extracto en estudio tiene efecto bactericida. Se concluye que el extracto hidroalcohólico de Portulaca oleracea L. presenta efecto inhibitorio in vitro
de tipo bactericida sobre el crecimiento de Staphylococcus aureus resistente a la meticilina.
In the present study, the in vitro effect of the hydroalcoholic extract of Portulaca oleracea L. at 0.625, 1.25, 2.5 and 5% on the growth of Staphylococcus aureus resistant to methicillin was determined. For this evaluation, five study groups were applied, one white group and four experimental ones, with which the effect of the hydroalcoholic extract of P. oleracea L. was evaluated using the diffusion technique in wells, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The phytoconstituents of the extract and their in vivo phytotoxicity were qualitatively identified by the percentage of mortality on Artemia salina nauplii. It was found that the increase in the concentration of the extract under study generated zone of inhibition of 13.07 ± 0.88, 17.33 ± 1.23, 24.40 ± 1.18 and 26.87 ± 1.41 mm in diameter; MIC of 12.5 mg/mL and MBC of 25 mg/mL. The qualitatively identified phytoconstituents were phenolic compounds, flavonoids, steroids and alkaloids, and the toxicity on Artemia salina nauplii was high. The statistical analysis shows that there is a significant difference (p < 0.01) between the white and experimental groups; as well as, between the experimental groups at the different concentrations under study; the CMB/CMI ratio indicates that the extract under study has a bactericidal effect. It is concluded that the hydroalcoholic extract of Portulaca oleracea L. has an in vitro inhibitory effect of a bactericidal type on the growth of Staphylococcus aureus resistant to methicillin.
In the present study, the in vitro effect of the hydroalcoholic extract of Portulaca oleracea L. at 0.625, 1.25, 2.5 and 5% on the growth of Staphylococcus aureus resistant to methicillin was determined. For this evaluation, five study groups were applied, one white group and four experimental ones, with which the effect of the hydroalcoholic extract of P. oleracea L. was evaluated using the diffusion technique in wells, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The phytoconstituents of the extract and their in vivo phytotoxicity were qualitatively identified by the percentage of mortality on Artemia salina nauplii. It was found that the increase in the concentration of the extract under study generated zone of inhibition of 13.07 ± 0.88, 17.33 ± 1.23, 24.40 ± 1.18 and 26.87 ± 1.41 mm in diameter; MIC of 12.5 mg/mL and MBC of 25 mg/mL. The qualitatively identified phytoconstituents were phenolic compounds, flavonoids, steroids and alkaloids, and the toxicity on Artemia salina nauplii was high. The statistical analysis shows that there is a significant difference (p < 0.01) between the white and experimental groups; as well as, between the experimental groups at the different concentrations under study; the CMB/CMI ratio indicates that the extract under study has a bactericidal effect. It is concluded that the hydroalcoholic extract of Portulaca oleracea L. has an in vitro inhibitory effect of a bactericidal type on the growth of Staphylococcus aureus resistant to methicillin.
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Keywords
Bactericida , Efecto bactericida, Extracto hidroalcohólico, Plantas comestibles