Actividad micoparasítica de cuatro cepas de Trichoderma spp. frente a Fusarium oxysporum, aisladas de Ananas comosus var. Roja Trujillana de Poroto, La Libertad
No Thumbnail Available
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
El objetivo de la presente investigación fue evaluar la capacidad antagónica de cuatro cepas endófitas de Trichoderma spp. (TP1, TP2, TP3 y TP4), obtenidas de piña (Ananas comosus) variedad Roja Trujillana en Poroto La Libertad. La actividad antagónica se evaluó frente a cuatro aislamientos de Fusarium oxysporum (F4A, F4C, FR1 y F6A), procedentes del tejido vascular de plantas de piña con estrías necróticas y marchitez. Para ello, se empleó la técnica de cultivo dual en placas de Petri con medio a base de papa – dextrosa y agar (PDA), cada cepa de Trichoderma spp. fue enfrentada con cada uno de los aislamientos de Fusarium oxysporum ; considerándose cada enfrentamiento como un tratamiento y se realizó 6 repeticiones con cada uno de ellos. Posteriormente, los tratamientos se ubicaron en el laboratorio bajo un diseño completo al azar, se hizo un registro diario de la temperatura y humedad del ambiente. Se evaluó la velocidad de crecimiento, halo de inhibición, parasitismo y competencia durante 14 días. Tres de las cepas de Trichoderma spp. inhibieron por antibiosis el desarrollo de todos los aislamientos de F. oxysporum a excepción de la cepa de Trichoderma spp. codificada como TP4 que presentó actividad micoparasítica frente a todos los aislamientos de F. oxysporum evaluados.
ABSTRACT The objective of the present investigation was to evaluate the antagonistic capacity of four endophytic strains of Trichoderma spp. (TP1, TP2, TP3 and TP4), obtained from pineapple (Ananas comosus) variety Red Trujillana in Poroto La Libertad. Antagonistic activity was evaluated against four isolates of Fusarium oxysporum (F4A, F4C, FR1 and F6A), from the vascular tissue of pineapple plants with necrotic striae and wilt. To do this, used the technique of dual culture in Petri dishes with medium based on potato dextrose and agar (PDA), each strain of Trichoderma spp. was faced with each of the isolates of Fusarium oxysporum; considering each confrontation as a treatment and 6 repetitions were made with each one of them. Subsequently, the treatments were located in the laboratory under a complete random design, a daily record of the temperature and humidity of the environment was made. The growth rate, inhibition zone, parasitism and competition were evaluated during 14 days. Three of the strains of Trichoderma spp. inhibited by antibiosis the development of all the isolates of F. oxysporum with the exception of the Trichoderma spp. coded as TP4 that showed mycoparasitic activity against all the isolates of F. oxysporum evaluated.
ABSTRACT The objective of the present investigation was to evaluate the antagonistic capacity of four endophytic strains of Trichoderma spp. (TP1, TP2, TP3 and TP4), obtained from pineapple (Ananas comosus) variety Red Trujillana in Poroto La Libertad. Antagonistic activity was evaluated against four isolates of Fusarium oxysporum (F4A, F4C, FR1 and F6A), from the vascular tissue of pineapple plants with necrotic striae and wilt. To do this, used the technique of dual culture in Petri dishes with medium based on potato dextrose and agar (PDA), each strain of Trichoderma spp. was faced with each of the isolates of Fusarium oxysporum; considering each confrontation as a treatment and 6 repetitions were made with each one of them. Subsequently, the treatments were located in the laboratory under a complete random design, a daily record of the temperature and humidity of the environment was made. The growth rate, inhibition zone, parasitism and competition were evaluated during 14 days. Three of the strains of Trichoderma spp. inhibited by antibiosis the development of all the isolates of F. oxysporum with the exception of the Trichoderma spp. coded as TP4 that showed mycoparasitic activity against all the isolates of F. oxysporum evaluated.
Description
Keywords
Endófito, cultivo dual, Inhibición, Parasitismo