Efecto del número de capas de ZnO en películas delgadas sobre el crecimiento de Escherichia coli y Staphylococcus aureus
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Date
2023
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Universidad Nacional de Trujillo
Abstract
En el presente trabajo de investigación, se determinó el efecto del número de capas de ZnO en películas delgadas sobre el crecimiento de Escherichia coli y Staphylococcus aureus. El material químico fue proporcionado por el Grupo Multidisciplinario de Investigación en Nanociencia y Nanotecnología de la Universidad Nacional de Trujillo (GMIN-UNT). Se utilizaron cepas de Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922 proporcionadas por el Laboratorio Referencial La Libertad. Para la determinación de la inhibición del crecimiento bacteriano se usó el método de placa vertida, colocando en cada placa Petri estéril 3 discos de 3,5,7 y 9 capas de películas delgadas de ZnO, y dos testigos (disco de vidrio sin película delgada de ZnO y disco de Ciprofloxacino), se realizaron seis repeticiones en ambas bacterias y se incubaron a 37ºC durante 24 horas. Posteriormente se realizó la medición de los diámetros de los halos de inhibición, para E. coli y S. aureus observándose que las películas delgadas de ZnO de 9 capas presentó el mayor valor respecto a las demás capas de 3,5 y 7 con un promedio para E. coli 15.67 mm y S. aureus 19.92 mm. Se concluye que a mayor número de capas de ZnO en películas delgadas presentan mayor efecto inhibitorio del crecimiento en ambas bacterias, siendo la película de 9 capas la que presentó mayor efecto inhibitorio, identificando a su vez que S. aureus es más susceptible a comparación con E. coli.
In the present research work, the effect of the number of layers of ZnO in thin films on the growth of Escherichia coli and Staphylococcus aureus was determined. The chemical material was provided by the Multidisciplinary Research Group in Nanoscience and Nanotechnology of the National University of Trujillo (GMIN-UNT). Staphylococcus aureus strains ATCC 25923 and Escherichia coli ATCC 25922 provided by the La Libertad Reference Laboratory were used. For the determination of bacterial growth inhibition, the poured dish method was used, placing in each sterile Petri dish 4 discs with 3,5,7 and 9 layers of thin films of ZnO, and two controls (glass disc without thin film of ZnO and Ciprofloxacin disc), with six repeats in both bacteria and incubated at 37ºC for 24 hours. Subsequently, the diameters of the inhibition halos were measured for E. coli and S. aureus, observing that thin films of ZnO of 9 layers presented the highest value compared to the other layers of 3,5, and 7; with an average for E. coli 15.67 mm and S. aureus 19.92 mm. It is concluded that the greater the number of layers of ZnO in thin films present greater inhibitory effect of growth in both bacteria, being the film of 9 layers the one that presented greater inhibitory effect, identifying in turn that S. aureus is more susceptible compared to E. coli.
In the present research work, the effect of the number of layers of ZnO in thin films on the growth of Escherichia coli and Staphylococcus aureus was determined. The chemical material was provided by the Multidisciplinary Research Group in Nanoscience and Nanotechnology of the National University of Trujillo (GMIN-UNT). Staphylococcus aureus strains ATCC 25923 and Escherichia coli ATCC 25922 provided by the La Libertad Reference Laboratory were used. For the determination of bacterial growth inhibition, the poured dish method was used, placing in each sterile Petri dish 4 discs with 3,5,7 and 9 layers of thin films of ZnO, and two controls (glass disc without thin film of ZnO and Ciprofloxacin disc), with six repeats in both bacteria and incubated at 37ºC for 24 hours. Subsequently, the diameters of the inhibition halos were measured for E. coli and S. aureus, observing that thin films of ZnO of 9 layers presented the highest value compared to the other layers of 3,5, and 7; with an average for E. coli 15.67 mm and S. aureus 19.92 mm. It is concluded that the greater the number of layers of ZnO in thin films present greater inhibitory effect of growth in both bacteria, being the film of 9 layers the one that presented greater inhibitory effect, identifying in turn that S. aureus is more susceptible compared to E. coli.
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Keywords
Películas delgadas de ZnO, Escherichia coli, Staphylococcus aureus, Inhibición, Crecimiento