Efecto de la melaza de caña de azúcar, glicerol y sulfato de amonio en la producción de biomasa, contenido de lípidos y cinética de crecimiento de Rhodosporidium toruloides
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Date
2021
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Publisher
Universidad Nacional de Trujillo
Abstract
La utilización de levaduras oleaginosas como fuente alternativa de aceite de oleoquimicos y biodiésel es de sumo interés, en este sentido, la búsqueda de sustratos disponibles que proporcionen elevado rendimiento y bajo costo, es importante. El propósito de este estudio fue evaluar el efecto de los azucares reductores de melaza de caña de azúcar (ARM), glicerol (Gli) y sulfato de amonio (SA) en la producción biomasa (ϕg), contenido de lípidos (L) y cinética de crecimiento de Rhodosporidium toruloides CCT 0783 NCYC. Se empleó un Diseño Compuesto Central Rotacional (DCCR), realizándose 18 ensayos, con ARM, Gli y SA entre 0.0 a 66.49 g/L, 0.0 y 50.0 g/L y 0.1 a 4.76 g/L respectivamente. Los cultivos se realizaron en matraces de 250 mL de capacidad con un volumen de medio de cultivo de 99 mL e inóculo de 1 mL a 250 rpm y temperatura de 28°C, por un tiempo de 48 h. El mayor contenido de L y ϕg fue 52.37% y 4.487 g/L respectivamente con T11, utilizando 33.24 g/L, y 2.43 g/L de ARM, y SA respectivamente, con una relación C/N de 29.32. La utilización del modelo de Gompertz, no permitió obtener una consistencia estadística de los datos experimentales (R2 entre 0.76 a 0.93), debido a la fase de adaptación de las células de R. toruloides al medio de cultivo y una alta dispersión de la cinética de crecimiento.
ABSTRACT The use of oleaginous yeasts as an alternative source of oleochemical oil and biodiesel is of great interest, in this sense, the search for available substrates that provide high performance and low cost is important. The purpose of this study was to evaluate the effect of sugarcane molasses reducing sugars (MSR), glycerol (Gly) and ammonium sulfate (AS) on biomass production (ϕg), lipid content (L) and Rhodosporidium toruloides CCT 0783 NCYC growth kinetics. A Rotational Central Composite Design (RCDD) was used, performing 18 tests, with MRS, Gly and AS between 0.0 to 66.49 g/L, 0.0 and 50.0 g/L and 0.1 to 4.76 g/L respectively. The cultures were carried out in 250 mL flasks with a volume of 99 mL culture medium and inoculum of 1 mL at 250 rpm and a temperature of 28 ° C, for a time of 48 h. The highest content of L and ϕg was 52.37% and 4.487 g/L respectively with T11, using 33.24 g/L, and 2.43 g/L of MSR, and AS respectively, with a C/N ratio of 29.32. The use of the Gompertz model did not allow obtaining a statistical consistency of the experimental data (R2 between 0.76 to 0.93), due to the adaptation phase of R. toruloides cells to the culture medium and a high dispersion of growth kinetics.
ABSTRACT The use of oleaginous yeasts as an alternative source of oleochemical oil and biodiesel is of great interest, in this sense, the search for available substrates that provide high performance and low cost is important. The purpose of this study was to evaluate the effect of sugarcane molasses reducing sugars (MSR), glycerol (Gly) and ammonium sulfate (AS) on biomass production (ϕg), lipid content (L) and Rhodosporidium toruloides CCT 0783 NCYC growth kinetics. A Rotational Central Composite Design (RCDD) was used, performing 18 tests, with MRS, Gly and AS between 0.0 to 66.49 g/L, 0.0 and 50.0 g/L and 0.1 to 4.76 g/L respectively. The cultures were carried out in 250 mL flasks with a volume of 99 mL culture medium and inoculum of 1 mL at 250 rpm and a temperature of 28 ° C, for a time of 48 h. The highest content of L and ϕg was 52.37% and 4.487 g/L respectively with T11, using 33.24 g/L, and 2.43 g/L of MSR, and AS respectively, with a C/N ratio of 29.32. The use of the Gompertz model did not allow obtaining a statistical consistency of the experimental data (R2 between 0.76 to 0.93), due to the adaptation phase of R. toruloides cells to the culture medium and a high dispersion of growth kinetics.
Description
Keywords
Rhodosporidium toruloides, Melaza de caña, Glicerol, Sulfato de amonio, Biodiésel, Oleoquímicos, Parámetros cinéticos