Efecto del extracto hidroalcohólico de Tropaeolum tuberosum Ruiz & Pavón. frente a estrés oxidativo inducido por acrilamida en hígado y cerebro de Mus musculus BALB/c
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Date
2022
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Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue determinar el efecto antioxidante del extracto hidroalcohólico (EH) de Tropaeolum tuberosum Ruiz & Pavón “mashua” frente a estrés oxidativo (EO) inducido por acrilamida en hígado y cerebro de Mus musculus BALB/c. Se formaron cinco grupos experimentales de cinco individuos machos cada uno: control negativo (CN) tratado con agua destilada estéril (ADE), control positivo (CP) tratado con acrilamida (50 mg/Kg de p.c.) más ADE, grupo estándar farmacológico (GES) tratado con acrilamida (50 mg/Kg de p.c.) más silimarina, (200 mg/Kg p.c.), grupo experimental GE50 tratado con acrilamida (50 mg/Kg de p.c.) más 50 mg/Kg p.c. de extracto de T. tuberosum y el grupo GE200 tratado con acrilamida (50 mg/Kg de p.c.) más extracto de T. tuberosum (200 mg/Kg p.c.). Se midió el EO en hígado y cerebro con el método TABRS; los resultados fueron sometidos a prueba ANOVA y Tukey, ambas con un 95% de confianza. Los resultados de los diferentes grupos en hígado y cerebro, respectivamente, se expresan en μg MDA/g de tejido de la siguiente manera: GCN con 0,14750±0,030 y 0,25705±0,035; GCP con 0,30725±0,015 y 0,48857±0,049; GES con 0,21943±0,029 y 0,25230±0,058; GE50 con 0,21943±0,029 y 0,44093±0,028; GE200 con 0,15755±0,014 y 0,19915±0,047. Se concluyó que la dosis de 200 mg/Kg p.c. del EH de T. tuberosum, tiene mayor efecto protector antioxidante que en GE50 y GES en hígado y cerebro respectivamente, lo cual fue corroborado en la histopatología hepática.
The aim of this research was to determine the antioxidant effect of hydroalcoholic extract (HE) of Tropaeolum tuberosum Ruiz & Pavón “mashua” against oxidative stress (OS) induced by acrylamide in liver and brain of Mus musculus BALB/c. Five experimental groups of five male individuals each were formed: negative control (NCG) treated with sterile distilled water (SDE), positive control (PCG) treated with acrylamide (50 mg/Kg of b.w.) plus SDE, pharmacological standard group (SEG) treated with acrylamide (50 mg/Kg p.c.) plus silymarin, (200 mg/Kg b.w.), experimental group EG50 treated with acrylamide (50 mg/Kg p.c.) plus 50 mg/Kg b.w. of T. tuberosum extract and the EG200 group treated with acrylamide (50 mg/Kg b.w.) plus T. tuberosum extract (200 mg/Kg b.w.). OS in liver and brain was measured with the TABRS method; the results were subjected to ANOVA and Tukey tests, both with 95% confidence. The results of the different groups in liver and brain, respectively, are expressed in μgMDA/g of tissue as follows: NCG with 0.14750±0.030 and 0.25705±0.035; PCG with 0.30725±0.015 and 0.48857±0.049; SEG with 0.21943±0.029 and 0.25230±0.058; EG50 with 0.21943±0.029 and 0.44093±0.028; EG200 with 0.15755±0.014 and 0.19915±0.047; demonstrated that the dose of 200 mg/Kg b.w. of T. tuberosum HE, has a greater antioxidant protective effect than EG50 and SEG in liver and brain, respectively, corroborated in liver histopathology.
The aim of this research was to determine the antioxidant effect of hydroalcoholic extract (HE) of Tropaeolum tuberosum Ruiz & Pavón “mashua” against oxidative stress (OS) induced by acrylamide in liver and brain of Mus musculus BALB/c. Five experimental groups of five male individuals each were formed: negative control (NCG) treated with sterile distilled water (SDE), positive control (PCG) treated with acrylamide (50 mg/Kg of b.w.) plus SDE, pharmacological standard group (SEG) treated with acrylamide (50 mg/Kg p.c.) plus silymarin, (200 mg/Kg b.w.), experimental group EG50 treated with acrylamide (50 mg/Kg p.c.) plus 50 mg/Kg b.w. of T. tuberosum extract and the EG200 group treated with acrylamide (50 mg/Kg b.w.) plus T. tuberosum extract (200 mg/Kg b.w.). OS in liver and brain was measured with the TABRS method; the results were subjected to ANOVA and Tukey tests, both with 95% confidence. The results of the different groups in liver and brain, respectively, are expressed in μgMDA/g of tissue as follows: NCG with 0.14750±0.030 and 0.25705±0.035; PCG with 0.30725±0.015 and 0.48857±0.049; SEG with 0.21943±0.029 and 0.25230±0.058; EG50 with 0.21943±0.029 and 0.44093±0.028; EG200 with 0.15755±0.014 and 0.19915±0.047; demonstrated that the dose of 200 mg/Kg b.w. of T. tuberosum HE, has a greater antioxidant protective effect than EG50 and SEG in liver and brain, respectively, corroborated in liver histopathology.
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Keywords
Tropaeolum tuberosum, Mashua, Capacidad antioxidante, Acrilamida, Radicales libres