Efecto del método de desinfección, medio de cultivo y reguladores de crecimiento en la introducción in vitro de Vaccinium corymbosum var. Biloxi a partir de segmentos nodales
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Date
2018
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Universidad Nacional de Trujillo
Abstract
Existe dentro de la técnica de micropropagación del arándano, la dificultad para establecer in vitro material proveniente de campo debido a la alta tasa de contaminación. Con el objetivo de evaluar el método de desinfección, medio de cultivo y reguladores de crecimiento en la introducción in vitro de Vaccinium corymbosum var. Biloxi a partir de segmentos nodales, se empleó el Diseño de Bloques Completo al Azar (DCA) con ocho tratamientos (combinaciones de inmersión en benomilo o tetraciclina, medio MS o WPM y adición de 2ip o AG3 al medio), tres bloques y diez repeticiones por tratamiento. Para ello, segmentos nodales desinfectados en el proceso anterior, de 2 cm de largo con un nudo fueron inoculados en el medio de cultivo básico descrito según los tratamientos. Cada unidad experimental consistió de un vial de 10 ml con 1 ml de medio de cultivo líquido. Se plantaron 10 repeticiones por tratamiento en tres bloques. El material cultivado se colocó en el cuarto de crecimiento a una temperatura de 21º C, con un fotoperiodo de 12 horas y 850 lux de intensidad lumínica. Los resultados de cada experimento fueron analizados mediante el ANVA al 95% de confianza para determinar diferencias estadísticas entre los tratamientos, donde se encontró significancia estadística se compararon las medias de los tratamientos mediante la diferencia mínima significativa en la prueba de Duncan. La combinación de antimicrobiano, medio de cultivo y regulador de crecimiento de los tratamientos T2 (medio MS + 2 ppm de AG3) y T3 (medio WPM + 2.5 ppm de 2ip) presentan el mayor número de yemas brotadas y baja tasa de oxidación sin embargo presentan una alta tasa de contaminación por hongos.
ABSTRACT Exist within the technique of micropropagation of the bilberry, the difficulty to establish in material originating vitro of field due to the loud rate of contamination. For the sake of evaluating the method of disinfection, I mediate of cultivation and regulators of growth in the introduction in Vaccinium's vitro corymbosum var. Biloxi as from nodal segments, used Complete Bloques's Design himself at random (DCA) with eight treatments (combinations of immersion in benomilo or tetraciclina, mediate MS or WPM and addition of 2ip or AG3 to the midway), three blocks and ten repetitions for treatment. For it, the nodal segments disinfected in the previous process, of 2 cm in length with a knot were inoculated in the midway of basic cultivation described according to the treatments. Each experimental unit consisted of 10 ml's ampoule with 1 ml of means of liquid cultivation. They set up 10 repetitions for treatment at three blocks. The cultivated material placed itself in the quarter of growth to a temperature of 21 C, with a fotoperiodo of 12 hours and 850 lux of radiant intensity. The results out of every experiment were examined by means of the ANVA to 95 % of confidence to determine statistical differences between the treatments, where significancia found itself statistical the stockings of the intervening treatments compared the minimal significant difference in Duncan's proof themselves. The combination of antimicrobial, average of cultivation and regulator of growth of the treatments T2 (half a MS + 2 ppm of AG3) and T3 (half a WPM + 2,5 ppm of 2ip) present the bigger number of sprouted yolks and it reduces rate of oxidation however they present a loud rate of contamination for mushrooms.
ABSTRACT Exist within the technique of micropropagation of the bilberry, the difficulty to establish in material originating vitro of field due to the loud rate of contamination. For the sake of evaluating the method of disinfection, I mediate of cultivation and regulators of growth in the introduction in Vaccinium's vitro corymbosum var. Biloxi as from nodal segments, used Complete Bloques's Design himself at random (DCA) with eight treatments (combinations of immersion in benomilo or tetraciclina, mediate MS or WPM and addition of 2ip or AG3 to the midway), three blocks and ten repetitions for treatment. For it, the nodal segments disinfected in the previous process, of 2 cm in length with a knot were inoculated in the midway of basic cultivation described according to the treatments. Each experimental unit consisted of 10 ml's ampoule with 1 ml of means of liquid cultivation. They set up 10 repetitions for treatment at three blocks. The cultivated material placed itself in the quarter of growth to a temperature of 21 C, with a fotoperiodo of 12 hours and 850 lux of radiant intensity. The results out of every experiment were examined by means of the ANVA to 95 % of confidence to determine statistical differences between the treatments, where significancia found itself statistical the stockings of the intervening treatments compared the minimal significant difference in Duncan's proof themselves. The combination of antimicrobial, average of cultivation and regulator of growth of the treatments T2 (half a MS + 2 ppm of AG3) and T3 (half a WPM + 2,5 ppm of 2ip) present the bigger number of sprouted yolks and it reduces rate of oxidation however they present a loud rate of contamination for mushrooms.
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Keywords
Cultivo, Arándano