Síntesis y caracterización de nanopartícula de sílice con ácido clorogénico y evaluación de su capacidad antioxidante
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Debido a la baja biodisponibilidad, y por ende una baja eficacia de los fármacos, se ha optado por desarrollar nuevas formas de moléculas junto con la nanotecnología, para así obtener partículas capaces de servir como medios de transporte para otras moléculas. Pues así, este estudio tiene como objetivo sintetizar y caracterizar nanopartículas de sílice con ácido clorogénico y evaluar su capacidad antioxidante, en donde se describe la síntesis de nanopartículas de sílice mediante el método de Stöber modificado, usando como precursor de las nanopartículas de sílice al Tetraetilo ortosilicato (TEOS). En la naturaleza, hay varios compuestos químicos con destacadas características antioxidantes, y uno de estos es el ácido clorogénico. Para valorar esta característica, se realizó una evaluación mediante reacciones químicas, siendo una de ellas con el 2,2-difenil-1-picrilhidracilo (DPPH). Luego, se realizó una medición inmediata en el UV a 517nm con etanol absoluto, con el objetivo de evaluar la capacidad antioxidante. Para caracterizar y determinar la morfología de dichos compuestos se realizó mediante técnicas como la espectroscopía infrarroja de Fourier (FTIR), donde identificamos los grupo hidroxilo, silanol y carbonilo; que tienen una buena estabilidad coloidal debido a su potencial Z; se usó también el método de microscopía electrónica de barrido (SEM) y la dispersión dinámica de luz (DLS), donde se muestra una distribución de nanopartículas esféricas de tamaño de 400 a 580 nm, uniformes en tamaño y dispersas sobre la superficie. Finalmente, del análisis de resultados se concluye que la síntesis de nanopartículas de sílice con ácido clorogénico fue exitoso y al evaluar su capacidad antioxidante se obtuvo un porcentaje de inhibición del 15%.
ABSTRACT Due to the low bioavailability and, therefore, low efficacy of drugs, it has been decided to develop new forms of molecules together with nanotechnology, in order to obtain particles capable of serving as transport media for other molecules. Thus, this study aims to synthesize and characterize silica nanoparticles with chlorogenic acid and evaluate their antioxidant capacity, where the synthesis of silica nanoparticles is described by the modified Stöber method, using Tetraethyl orthosilicate (TEOS) as a precursor of silica nanoparticles. In nature, there are several chemical compounds with outstanding antioxidant characteristics, and one of these is chlorogenic acid. To assess this characteristic, an evaluation was carried out through chemical reactions, one of them being with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Then, an immediate measurement was made in the UV at 517nm with absolute ethanol, in order to evaluate the antioxidant capacity. To characterize and determine the morphology of these compounds, techniques such as Fourier infrared spectroscopy (FTIR) were used, where we identified the hydroxyl, silanol and carbonyl groups; which have a good colloidal stability due to their Z potential; the scanning electron microscopy (SEM) method and dynamic light scattering (DLS) were also used, where a distribution of spherical nanoparticles of size 579 nm, uniform in size and dispersed on the surface, is shown. Finally, from the analysis of results it is concluded that the synthesis of silica nanoparticles with chlorogenic acid was successful and when evaluating its antioxidant capacity, an inhibition percentage of 15% was obtained.
ABSTRACT Due to the low bioavailability and, therefore, low efficacy of drugs, it has been decided to develop new forms of molecules together with nanotechnology, in order to obtain particles capable of serving as transport media for other molecules. Thus, this study aims to synthesize and characterize silica nanoparticles with chlorogenic acid and evaluate their antioxidant capacity, where the synthesis of silica nanoparticles is described by the modified Stöber method, using Tetraethyl orthosilicate (TEOS) as a precursor of silica nanoparticles. In nature, there are several chemical compounds with outstanding antioxidant characteristics, and one of these is chlorogenic acid. To assess this characteristic, an evaluation was carried out through chemical reactions, one of them being with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Then, an immediate measurement was made in the UV at 517nm with absolute ethanol, in order to evaluate the antioxidant capacity. To characterize and determine the morphology of these compounds, techniques such as Fourier infrared spectroscopy (FTIR) were used, where we identified the hydroxyl, silanol and carbonyl groups; which have a good colloidal stability due to their Z potential; the scanning electron microscopy (SEM) method and dynamic light scattering (DLS) were also used, where a distribution of spherical nanoparticles of size 579 nm, uniform in size and dispersed on the surface, is shown. Finally, from the analysis of results it is concluded that the synthesis of silica nanoparticles with chlorogenic acid was successful and when evaluating its antioxidant capacity, an inhibition percentage of 15% was obtained.
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Keywords
Nanotecnología. Nanopartículas. Sílice. Acido clorogénico. Capacidad antioxidante.